BMRB Entry 5312
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5312
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gutierrez, Pablo; Coillet-Matillon, Stephane; Arrowsmith, Cheryl; Gehring, Kalle. "Zinc is required for Structural Stability of the C-terminus of Archaeal
Translation Initiation Factor aIF2beta " FEBS Lett. 517, 155-158 (2002).
PubMed: 12062427
Assembly members:
translation initiation factor 2 beta, polymer, 135 residues, 16200 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Methanothermobacter thermautotrophicus Taxonomy ID: 145262 Superkingdom: Archaea Kingdom: not available Genus/species: Methanobacterium thermoautotrophicum
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
translation initiation factor 2 beta: MDDYEKLLERAIDQLPPEVF
ETKRFEVPKAYSVIQGNRTF
IQNFREVADALNRDPQHLLK
FLLRELGTAGNLEGGRAILQ
GKFTHFLINERIEDYVNKFV
ICHECNRPDTRIIREGRISL
LKCEACGAKAPLKNV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 518 |
| 13C chemical shifts | 296 |
| 15N chemical shifts | 122 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | aIF2beta | 1 |
| 2 | Zinc ion | 2 |
Entities:
Entity 1, aIF2beta 135 residues - 16200 Da.
| 1 | MET | ASP | ASP | TYR | GLU | LYS | LEU | LEU | GLU | ARG | ||||
| 2 | ALA | ILE | ASP | GLN | LEU | PRO | PRO | GLU | VAL | PHE | ||||
| 3 | GLU | THR | LYS | ARG | PHE | GLU | VAL | PRO | LYS | ALA | ||||
| 4 | TYR | SER | VAL | ILE | GLN | GLY | ASN | ARG | THR | PHE | ||||
| 5 | ILE | GLN | ASN | PHE | ARG | GLU | VAL | ALA | ASP | ALA | ||||
| 6 | LEU | ASN | ARG | ASP | PRO | GLN | HIS | LEU | LEU | LYS | ||||
| 7 | PHE | LEU | LEU | ARG | GLU | LEU | GLY | THR | ALA | GLY | ||||
| 8 | ASN | LEU | GLU | GLY | GLY | ARG | ALA | ILE | LEU | GLN | ||||
| 9 | GLY | LYS | PHE | THR | HIS | PHE | LEU | ILE | ASN | GLU | ||||
| 10 | ARG | ILE | GLU | ASP | TYR | VAL | ASN | LYS | PHE | VAL | ||||
| 11 | ILE | CYS | HIS | GLU | CYS | ASN | ARG | PRO | ASP | THR | ||||
| 12 | ARG | ILE | ILE | ARG | GLU | GLY | ARG | ILE | SER | LEU | ||||
| 13 | LEU | LYS | CYS | GLU | ALA | CYS | GLY | ALA | LYS | ALA | ||||
| 14 | PRO | LEU | LYS | ASN | VAL |
Entity 2, Zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: translation initiation factor 2 beta, [U-13C; U-15N], 1.0 – 2.0 mM; ZINC (II) ION 51 mM
sample_2: translation initiation factor 2 beta, [U-15N], 1.0 – 2.0 mM; ZINC (II) ION 51 mM
condition_1: pH: 6.0 na; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | condition_1 |
| 1H-15N TOCSY | not available | not available | condition_1 |
| HNCA | not available | not available | condition_1 |
| HNCACB | not available | not available | condition_1 |
| CBCA(CO)NH | not available | not available | condition_1 |
| 1H-13C NOESY | not available | not available | condition_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
- Varian UNITYplus 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks