BMRB Entry 5311

Title:
Letter to the Editor: 1H, 15N and 13C assignments of the Pseudomonas effector protein, AvrPto
Deposition date:
2002-03-06
Original release date:
2002-08-23
Authors:
Wulf, Jennifer; Pascuzzi, Pete; Martin, Gregory; Nicholson, Linda
Citation:

Citation: Wulf, Jennifer; Pascuzzi, Pete; Martin, Gregory; Nicholson, Linda. "Letter to the Editor: 1H, 15N and 13C assignments of the Pseudomonas effector protein, AvrPto "  J. Biomol. NMR 23, 247-248 (2002).

Assembly members:

Assembly members:
AvrPto, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas syringae   Taxonomy ID: 317   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas syringae

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts629
13C chemical shifts389
15N chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AvrPto subunit 11
2AvrPto subunit 21

Entities:

Entity 1, AvrPto subunit 1 105 residues - Formula weight is not available

1   ASPASNVALTHRSERSERGLNLEULEUSER
2   VALARGHISGLNLEUALAGLUSERALAGLY
3   LEUPROARGASPGLNHISGLUPHEVALSER
4   SERGLNALAPROGLNSERLEUARGASNARG
5   TYRASNASNLEUTYRSERHISTHRGLNARG
6   THRLEUASPMETALAASPMETGLNHISARG
7   TYRMETTHRGLYALASERGLYILEASNPRO
8   GLYMETLEUPROHISGLUASNVALASPASP
9   METARGSERALAILETHRASPTRPSERASP
10   METARGGLUALALEUGLNHISALAMETGLY
11   ILEHISALAASPILE

Samples:

sample_1: AvrPto, [U-15N], 1.0 mM

sample_2: AvrPto, [U-13C; U-15N], 1.0 mM

condition_all: pH: 6.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
2D homonuclear NOESYnot availablenot availablenot available
2D homonuclear TOCSYnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HC(CO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
1H-13C aromatic HSQCnot availablenot availablenot available
1H-13C HCCH-TOCSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
GB AAA25728 AAO57459 ABS87317 ABS87322 AHN52240
PRF 1916396A
REF NP_793764 WP_011104823

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks