BMRB Entry 53097

Name: Glucocorticoid receptor Activation Domain (GR AD)
Published: 2026-01-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53097

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Glucocorticoid receptor Activation Domain (GR AD)

Deposition date:

2025-05-05

Original release date:

2026-01-06

Authors:

Bielskute, Stase; Garcia, Jesus; Salvatella, Xavier

Citation:

Citation: Bielskute, Stase; Mateos, Borja; Awawdy, Muhammad; Garcia-Cabau, Carla; Niskanen, Henri; Sanchez-Zarzalejo, Carolina; Bracaglia, Lorenzo; Pierattelli, Roberta; Felli, Isabella; Frigole-Vivas, Marta; Garcia, Jesus; Riera, Antoni; Hnisz, Denes; Salvatella, Xavier. "Oligomerization enables the selective targeting of an intrinsically disordered region by a small molecule" Sci. Adv. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 420 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MDSKESLTPGREENPSSVLA QERGDVMDFYKTLRGGATVK VSASSPSLAVASQSDSKQRR LLVDFPKGSVSNAQQPDLSK AVSLSMGLYMGETETKVMGN DLGFPQQGQISLSSGETDLK LLEESIANLNRSTSVPENPK SSASTAVSAAPTEKEFPKTH SDVSSEQQHLKGQTGTNGGN VKLYTTDQSTFDILQDLEFS SGSPGKETNESPWRSDLLID ENCLLSPLAGEDDSFLLEGN SNEDCKPLILPDTKPKIKDN GDLVLSSPSNVTLPQVKTEK EDFIELCTPGVIKQEKLGTV YCQASFPGANIIGNKMSAIS VHGVSTSGGQMYHYDMNTAS LSQQQDQKPIFNVIPPIPVG SENWNRCQGSGDDNLTSLGT LNFPGRTVFSNGYSSPSMRP DVSSPPSSSSTATTGPPPKL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	1124
15N chemical shifts	357
1H chemical shifts	357

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GR AD	1

Entities:

Entity 1, GR AD 420 residues - Formula weight is not available

1	MET	ASP	SER	LYS	GLU	SER	LEU	THR	PRO	GLY
2	ARG	GLU	GLU	ASN	PRO	SER	SER	VAL	LEU	ALA
3	GLN	GLU	ARG	GLY	ASP	VAL	MET	ASP	PHE	TYR
4	LYS	THR	LEU	ARG	GLY	GLY	ALA	THR	VAL	LYS
5	VAL	SER	ALA	SER	SER	PRO	SER	LEU	ALA	VAL
6	ALA	SER	GLN	SER	ASP	SER	LYS	GLN	ARG	ARG
7	LEU	LEU	VAL	ASP	PHE	PRO	LYS	GLY	SER	VAL
8	SER	ASN	ALA	GLN	GLN	PRO	ASP	LEU	SER	LYS
9	ALA	VAL	SER	LEU	SER	MET	GLY	LEU	TYR	MET
10	GLY	GLU	THR	GLU	THR	LYS	VAL	MET	GLY	ASN
11	ASP	LEU	GLY	PHE	PRO	GLN	GLN	GLY	GLN	ILE
12	SER	LEU	SER	SER	GLY	GLU	THR	ASP	LEU	LYS
13	LEU	LEU	GLU	GLU	SER	ILE	ALA	ASN	LEU	ASN
14	ARG	SER	THR	SER	VAL	PRO	GLU	ASN	PRO	LYS
15	SER	SER	ALA	SER	THR	ALA	VAL	SER	ALA	ALA
16	PRO	THR	GLU	LYS	GLU	PHE	PRO	LYS	THR	HIS
17	SER	ASP	VAL	SER	SER	GLU	GLN	GLN	HIS	LEU
18	LYS	GLY	GLN	THR	GLY	THR	ASN	GLY	GLY	ASN
19	VAL	LYS	LEU	TYR	THR	THR	ASP	GLN	SER	THR
20	PHE	ASP	ILE	LEU	GLN	ASP	LEU	GLU	PHE	SER
21	SER	GLY	SER	PRO	GLY	LYS	GLU	THR	ASN	GLU
22	SER	PRO	TRP	ARG	SER	ASP	LEU	LEU	ILE	ASP
23	GLU	ASN	CYS	LEU	LEU	SER	PRO	LEU	ALA	GLY
24	GLU	ASP	ASP	SER	PHE	LEU	LEU	GLU	GLY	ASN
25	SER	ASN	GLU	ASP	CYS	LYS	PRO	LEU	ILE	LEU
26	PRO	ASP	THR	LYS	PRO	LYS	ILE	LYS	ASP	ASN
27	GLY	ASP	LEU	VAL	LEU	SER	SER	PRO	SER	ASN
28	VAL	THR	LEU	PRO	GLN	VAL	LYS	THR	GLU	LYS
29	GLU	ASP	PHE	ILE	GLU	LEU	CYS	THR	PRO	GLY
30	VAL	ILE	LYS	GLN	GLU	LYS	LEU	GLY	THR	VAL
31	TYR	CYS	GLN	ALA	SER	PHE	PRO	GLY	ALA	ASN
32	ILE	ILE	GLY	ASN	LYS	MET	SER	ALA	ILE	SER
33	VAL	HIS	GLY	VAL	SER	THR	SER	GLY	GLY	GLN
34	MET	TYR	HIS	TYR	ASP	MET	ASN	THR	ALA	SER
35	LEU	SER	GLN	GLN	GLN	ASP	GLN	LYS	PRO	ILE
36	PHE	ASN	VAL	ILE	PRO	PRO	ILE	PRO	VAL	GLY
37	SER	GLU	ASN	TRP	ASN	ARG	CYS	GLN	GLY	SER
38	GLY	ASP	ASP	ASN	LEU	THR	SER	LEU	GLY	THR
39	LEU	ASN	PHE	PRO	GLY	ARG	THR	VAL	PHE	SER
40	ASN	GLY	TYR	SER	SER	PRO	SER	MET	ARG	PRO
41	ASP	VAL	SER	SER	PRO	PRO	SER	SER	SER	SER
42	THR	ALA	THR	THR	GLY	PRO	PRO	PRO	LYS	LEU

Samples:

sample_1: GR AD, [U-100% 13C; U-100% 15N], 280 uM; D2O, [U-100% 2H], 10%; DSS 10 uM; sodium azide 0.05%; sodium phosphate 20 mM; TCEP 1 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)NNH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment, data analysis

TOPSPIN - collection

NMRPipe - processing

qMDD - processing

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	ASP	SER	LYS	GLU	SER	LEU	THR	PRO	GLY
2	ARG	GLU	GLU	ASN	PRO	SER	SER	VAL	LEU	ALA
3	GLN	GLU	ARG	GLY	ASP	VAL	MET	ASP	PHE	TYR
4	LYS	THR	LEU	ARG	GLY	GLY	ALA	THR	VAL	LYS
5	VAL	SER	ALA	SER	SER	PRO	SER	LEU	ALA	VAL
6	ALA	SER	GLN	SER	ASP	SER	LYS	GLN	ARG	ARG
7	LEU	LEU	VAL	ASP	PHE	PRO	LYS	GLY	SER	VAL
8	SER	ASN	ALA	GLN	GLN	PRO	ASP	LEU	SER	LYS
9	ALA	VAL	SER	LEU	SER	MET	GLY	LEU	TYR	MET
10	GLY	GLU	THR	GLU	THR	LYS	VAL	MET	GLY	ASN
11	ASP	LEU	GLY	PHE	PRO	GLN	GLN	GLY	GLN	ILE
12	SER	LEU	SER	SER	GLY	GLU	THR	ASP	LEU	LYS
13	LEU	LEU	GLU	GLU	SER	ILE	ALA	ASN	LEU	ASN
14	ARG	SER	THR	SER	VAL	PRO	GLU	ASN	PRO	LYS
15	SER	SER	ALA	SER	THR	ALA	VAL	SER	ALA	ALA
16	PRO	THR	GLU	LYS	GLU	PHE	PRO	LYS	THR	HIS
17	SER	ASP	VAL	SER	SER	GLU	GLN	GLN	HIS	LEU
18	LYS	GLY	GLN	THR	GLY	THR	ASN	GLY	GLY	ASN
19	VAL	LYS	LEU	TYR	THR	THR	ASP	GLN	SER	THR
20	PHE	ASP	ILE	LEU	GLN	ASP	LEU	GLU	PHE	SER
21	SER	GLY	SER	PRO	GLY	LYS	GLU	THR	ASN	GLU
22	SER	PRO	TRP	ARG	SER	ASP	LEU	LEU	ILE	ASP
23	GLU	ASN	CYS	LEU	LEU	SER	PRO	LEU	ALA	GLY
24	GLU	ASP	ASP	SER	PHE	LEU	LEU	GLU	GLY	ASN
25	SER	ASN	GLU	ASP	CYS	LYS	PRO	LEU	ILE	LEU
26	PRO	ASP	THR	LYS	PRO	LYS	ILE	LYS	ASP	ASN
27	GLY	ASP	LEU	VAL	LEU	SER	SER	PRO	SER	ASN
28	VAL	THR	LEU	PRO	GLN	VAL	LYS	THR	GLU	LYS
29	GLU	ASP	PHE	ILE	GLU	LEU	CYS	THR	PRO	GLY
30	VAL	ILE	LYS	GLN	GLU	LYS	LEU	GLY	THR	VAL
31	TYR	CYS	GLN	ALA	SER	PHE	PRO	GLY	ALA	ASN
32	ILE	ILE	GLY	ASN	LYS	MET	SER	ALA	ILE	SER
33	VAL	HIS	GLY	VAL	SER	THR	SER	GLY	GLY	GLN
34	MET	TYR	HIS	TYR	ASP	MET	ASN	THR	ALA	SER
35	LEU	SER	GLN	GLN	GLN	ASP	GLN	LYS	PRO	ILE
36	PHE	ASN	VAL	ILE	PRO	PRO	ILE	PRO	VAL	GLY
37	SER	GLU	ASN	TRP	ASN	ARG	CYS	GLN	GLY	SER
38	GLY	ASP	ASP	ASN	LEU	THR	SER	LEU	GLY	THR
39	LEU	ASN	PHE	PRO	GLY	ARG	THR	VAL	PHE	SER
40	ASN	GLY	TYR	SER	SER	PRO	SER	MET	ARG	PRO
41	ASP	VAL	SER	SER	PRO	PRO	SER	SER	SER	SER
42	THR	ALA	THR	THR	GLY	PRO	PRO	PRO	LYS	LEU

1	MET	ASP	SER	LYS	GLU	SER	LEU	THR	PRO	GLY
2	ARG	GLU	GLU	ASN	PRO	SER	SER	VAL	LEU	ALA
3	GLN	GLU	ARG	GLY	ASP	VAL	MET	ASP	PHE	TYR
4	LYS	THR	LEU	ARG	GLY	GLY	ALA	THR	VAL	LYS
5	VAL	SER	ALA	SER	SER	PRO	SER	LEU	ALA	VAL
6	ALA	SER	GLN	SER	ASP	SER	LYS	GLN	ARG	ARG
7	LEU	LEU	VAL	ASP	PHE	PRO	LYS	GLY	SER	VAL
8	SER	ASN	ALA	GLN	GLN	PRO	ASP	LEU	SER	LYS
9	ALA	VAL	SER	LEU	SER	MET	GLY	LEU	TYR	MET
10	GLY	GLU	THR	GLU	THR	LYS	VAL	MET	GLY	ASN
11	ASP	LEU	GLY	PHE	PRO	GLN	GLN	GLY	GLN	ILE
12	SER	LEU	SER	SER	GLY	GLU	THR	ASP	LEU	LYS
13	LEU	LEU	GLU	GLU	SER	ILE	ALA	ASN	LEU	ASN
14	ARG	SER	THR	SER	VAL	PRO	GLU	ASN	PRO	LYS
15	SER	SER	ALA	SER	THR	ALA	VAL	SER	ALA	ALA
16	PRO	THR	GLU	LYS	GLU	PHE	PRO	LYS	THR	HIS
17	SER	ASP	VAL	SER	SER	GLU	GLN	GLN	HIS	LEU
18	LYS	GLY	GLN	THR	GLY	THR	ASN	GLY	GLY	ASN
19	VAL	LYS	LEU	TYR	THR	THR	ASP	GLN	SER	THR
20	PHE	ASP	ILE	LEU	GLN	ASP	LEU	GLU	PHE	SER
21	SER	GLY	SER	PRO	GLY	LYS	GLU	THR	ASN	GLU
22	SER	PRO	TRP	ARG	SER	ASP	LEU	LEU	ILE	ASP
23	GLU	ASN	CYS	LEU	LEU	SER	PRO	LEU	ALA	GLY
24	GLU	ASP	ASP	SER	PHE	LEU	LEU	GLU	GLY	ASN
25	SER	ASN	GLU	ASP	CYS	LYS	PRO	LEU	ILE	LEU
26	PRO	ASP	THR	LYS	PRO	LYS	ILE	LYS	ASP	ASN
27	GLY	ASP	LEU	VAL	LEU	SER	SER	PRO	SER	ASN
28	VAL	THR	LEU	PRO	GLN	VAL	LYS	THR	GLU	LYS
29	GLU	ASP	PHE	ILE	GLU	LEU	CYS	THR	PRO	GLY
30	VAL	ILE	LYS	GLN	GLU	LYS	LEU	GLY	THR	VAL
31	TYR	CYS	GLN	ALA	SER	PHE	PRO	GLY	ALA	ASN
32	ILE	ILE	GLY	ASN	LYS	MET	SER	ALA	ILE	SER
33	VAL	HIS	GLY	VAL	SER	THR	SER	GLY	GLY	GLN
34	MET	TYR	HIS	TYR	ASP	MET	ASN	THR	ALA	SER
35	LEU	SER	GLN	GLN	GLN	ASP	GLN	LYS	PRO	ILE
36	PHE	ASN	VAL	ILE	PRO	PRO	ILE	PRO	VAL	GLY
37	SER	GLU	ASN	TRP	ASN	ARG	CYS	GLN	GLY	SER
38	GLY	ASP	ASP	ASN	LEU	THR	SER	LEU	GLY	THR
39	LEU	ASN	PHE	PRO	GLY	ARG	THR	VAL	PHE	SER
40	ASN	GLY	TYR	SER	SER	PRO	SER	MET	ARG	PRO
41	ASP	VAL	SER	SER	PRO	PRO	SER	SER	SER	SER
42	THR	ALA	THR	THR	GLY	PRO	PRO	PRO	LYS	LEU

1	MET	ASP	SER	LYS	GLU	SER	LEU	THR	PRO	GLY
2	ARG	GLU	GLU	ASN	PRO	SER	SER	VAL	LEU	ALA
3	GLN	GLU	ARG	GLY	ASP	VAL	MET	ASP	PHE	TYR
4	LYS	THR	LEU	ARG	GLY	GLY	ALA	THR	VAL	LYS
5	VAL	SER	ALA	SER	SER	PRO	SER	LEU	ALA	VAL
6	ALA	SER	GLN	SER	ASP	SER	LYS	GLN	ARG	ARG
7	LEU	LEU	VAL	ASP	PHE	PRO	LYS	GLY	SER	VAL
8	SER	ASN	ALA	GLN	GLN	PRO	ASP	LEU	SER	LYS
9	ALA	VAL	SER	LEU	SER	MET	GLY	LEU	TYR	MET
10	GLY	GLU	THR	GLU	THR	LYS	VAL	MET	GLY	ASN
11	ASP	LEU	GLY	PHE	PRO	GLN	GLN	GLY	GLN	ILE
12	SER	LEU	SER	SER	GLY	GLU	THR	ASP	LEU	LYS
13	LEU	LEU	GLU	GLU	SER	ILE	ALA	ASN	LEU	ASN
14	ARG	SER	THR	SER	VAL	PRO	GLU	ASN	PRO	LYS
15	SER	SER	ALA	SER	THR	ALA	VAL	SER	ALA	ALA
16	PRO	THR	GLU	LYS	GLU	PHE	PRO	LYS	THR	HIS
17	SER	ASP	VAL	SER	SER	GLU	GLN	GLN	HIS	LEU
18	LYS	GLY	GLN	THR	GLY	THR	ASN	GLY	GLY	ASN
19	VAL	LYS	LEU	TYR	THR	THR	ASP	GLN	SER	THR
20	PHE	ASP	ILE	LEU	GLN	ASP	LEU	GLU	PHE	SER
21	SER	GLY	SER	PRO	GLY	LYS	GLU	THR	ASN	GLU
22	SER	PRO	TRP	ARG	SER	ASP	LEU	LEU	ILE	ASP
23	GLU	ASN	CYS	LEU	LEU	SER	PRO	LEU	ALA	GLY
24	GLU	ASP	ASP	SER	PHE	LEU	LEU	GLU	GLY	ASN
25	SER	ASN	GLU	ASP	CYS	LYS	PRO	LEU	ILE	LEU
26	PRO	ASP	THR	LYS	PRO	LYS	ILE	LYS	ASP	ASN
27	GLY	ASP	LEU	VAL	LEU	SER	SER	PRO	SER	ASN
28	VAL	THR	LEU	PRO	GLN	VAL	LYS	THR	GLU	LYS
29	GLU	ASP	PHE	ILE	GLU	LEU	CYS	THR	PRO	GLY
30	VAL	ILE	LYS	GLN	GLU	LYS	LEU	GLY	THR	VAL
31	TYR	CYS	GLN	ALA	SER	PHE	PRO	GLY	ALA	ASN
32	ILE	ILE	GLY	ASN	LYS	MET	SER	ALA	ILE	SER
33	VAL	HIS	GLY	VAL	SER	THR	SER	GLY	GLY	GLN
34	MET	TYR	HIS	TYR	ASP	MET	ASN	THR	ALA	SER
35	LEU	SER	GLN	GLN	GLN	ASP	GLN	LYS	PRO	ILE
36	PHE	ASN	VAL	ILE	PRO	PRO	ILE	PRO	VAL	GLY
37	SER	GLU	ASN	TRP	ASN	ARG	CYS	GLN	GLY	SER
38	GLY	ASP	ASP	ASN	LEU	THR	SER	LEU	GLY	THR
39	LEU	ASN	PHE	PRO	GLY	ARG	THR	VAL	PHE	SER
40	ASN	GLY	TYR	SER	SER	PRO	SER	MET	ARG	PRO
41	ASP	VAL	SER	SER	PRO	PRO	SER	SER	SER	SER
42	THR	ALA	THR	THR	GLY	PRO	PRO	PRO	LYS	LEU