BMRB Entry 5309

Name: 1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex
Published: 2002-08-22

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PDB ID: 1m3v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5309
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex

Deposition date:

2002-03-04

Original release date:

2002-08-22

Authors:

Deane, Janet; Visvader, Jane; Mackay, Joel; Matthews, Jacqueline

Citation:

Citation: Deane, Janet; Visvader, Jane; Mackay, Joel; Matthews, Jacqueline. "Letter to the Editor: 1H, 15N and 13C assignments of FLIN4, an intramolecular LMO4:ldb1 complex" J. Biomol. NMR 23, 165-166 (2002).
PubMed: 12153047

Assembly members:

Assembly members:
engineered intramolecular protein complex, polymer, 122 residues, 13004 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
engineered intramolecular protein complex: GSLSWKRCAGCGGKIADRFL LYAMDSYWHSRCLKCSSCQA QLGDIGTSSYTKSGMILCRN DYIRLFGNSGAGGSGGHMGS GGDVMVVGEPTLMGGEFGDE DERLITRLENTQFDAANGID DE

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	393
15N chemical shifts	116
1H chemical shifts	693

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	flin4	1
2	Zinc ion, I	2
3	Zinc ion, II	2

Entities:

Entity 1, flin4 122 residues - 13004 Da.

1

GLY

SER

LEU

SER

TRP

LYS

ARG

CYS

ALA

GLY

2

CYS

GLY

LYS

ILE

ALA

ASP

ARG

PHE

LEU

3

LEU

TYR

ALA

MET

ASP

SER

TYR

TRP

HIS

SER

4

ARG

CYS

LEU

LYS

CYS

SER

CYS

GLN

ALA

5

GLN

LEU

GLY

ASP

ILE

GLY

THR

SER

TYR

6

THR

LYS

SER

GLY

MET

ILE

LEU

CYS

ARG

ASN

7

ASP

TYR

ILE

ARG

LEU

PHE

GLY

ASN

SER

GLY

8

ALA

GLY

SER

GLY

HIS

MET

GLY

SER

9

GLY

ASP

VAL

MET

VAL

GLY

GLU

PRO

10

THR

LEU

MET

GLY

GLU

PHE

GLY

ASP

GLU

11

ASP

GLU

ARG

LEU

ILE

THR

ARG

LEU

GLU

ASN

12

THR

GLN

PHE

ASP

ALA

ASN

GLY

ILE

ASP

13

ASP

GLU

Entity 2, Zinc ion, I - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: engineered intramolecular protein complex0.3 – 0.8 mM

sample_2: engineered intramolecular protein complex, [U-95% 15N], 0.3 – 0.8 mM

sample_3: engineered intramolecular protein complex, [U-95% 13C; U-95% 15N], 0.3 – 0.8 mM

cond_1: ionic strength: 0.22 M; pH: 7.0 na; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H TOCSY	not available	not available	cond_1
1H NOESY	not available	not available	cond_1
1H DQF-COSY	not available	not available	cond_1
1H-15N HSQC	not available	not available	cond_1
HNHA	not available	not available	cond_1
HNCA	not available	not available	cond_1
HN(CO)CA	not available	not available	cond_1
HNCACB	not available	not available	cond_1
CBCA(CO)NH	not available	not available	cond_1
HNCO	not available	not available	cond_1
HN(CA)CO	not available	not available	cond_1
CC(CO)NH-TOCSY	not available	not available	cond_1
HCC(CO)NH-TOCSY	not available	not available	cond_1
HCCH-TOCSY	not available	not available	cond_1
15N-NOESY-HSQC	not available	not available	cond_1

Software:

xwinnmr - data aquisition, processing

XEASY - assignments

NMR spectrometers:

BRUKER DMX 600 MHz