BMRB Entry 53085

Name: Assignment of the Lassa Virus (LASV) Fusion Domain (FD) in the Pre-Fusion State
Published: 2025-12-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53085

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of the Lassa Virus (LASV) Fusion Domain (FD) in the Pre-Fusion State

Deposition date:

2025-04-28

Original release date:

2025-12-26

Authors:

Pennington, Hallie; Lee, Jinwoo

Citation:

Citation: Pennington, Hallie; Lee, Jinwoo. "Acidic bicelles are a suitable membrane mimic for structural studies of the Lassa virus fusion domain" Biochim. Biophys. Acta Biomembr. 1867, 184428-184428 (2025).
PubMed: 40482873

Assembly members:

Assembly members:
entity_1, polymer, 36 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Lassa Mammarenavirus Taxonomy ID: 3052310 Superkingdom: Viruses Kingdom: not available Genus/species: Mammarenavirus lassaense

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GTFTWTLSDSEGKDTPGGYC LTRWMLIEAELKCFGN

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	208
15N chemical shifts	60
1H chemical shifts	60

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LASV FD	1

Entities:

Entity 1, LASV FD 36 residues - Formula weight is not available

1

GLY

THR

PHE

THR

TRP

THR

LEU

SER

ASP

SER

2

GLU

GLY

LYS

ASP

THR

PRO

GLY

TYR

CYS

3

LEU

THR

ARG

TRP

MET

LEU

ILE

GLU

ALA

GLU

4

LEU

LYS

CYS

PHE

GLY

ASN

Samples:

sample_1: LASV FD, [U-13C; U-15N; U-2H], 650 uM; sodium phosphate 25 mM; sodium chloride 100 mM

sample_conditions_1: pH: 7.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRbox - processing

NMRFAM-SPARKY - chemical shift assignment

TALOS+ - data analysis

NMR spectrometers:

Bruker AVANCE III 800 MHz