BMRB Entry 53037

Name: Myc interacting domain 2 (Mid2) of Miz1 (ZBTB17)
Published: 2025-04-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53037

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Myc interacting domain 2 (Mid2) of Miz1 (ZBTB17)

Deposition date:

2025-04-15

Original release date:

2025-04-30

Authors:

Lavigne, Pierre; Boisvert, Olivier

Citation:

Citation: Boisvert, Olivier; Boisvert, Jonathan; Letourneau, Danny; Lavigne, Pierre. "Backbone NMR assignment of Myc Interacting Domain (MID2) of Miz-1 (ZBTB17)" Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET-3A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHQGKAGIKILEPEEGSEVS VVTVDDMVTLATEALAATAV TQLTVVPVGAAVTADETEVL KAEISKAVKQVQEEDPNTHI LYHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	300
15N chemical shifts	71
1H chemical shifts	141

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Mid2	1

Entities:

Entity 1, Mid2 88 residues - Formula weight is not available

From uniprot entry Q13105 (ZBTB17_HUMAN), the first Histidine of the polymer sequence corresponds to residue 637 of the full protein.

1

MET

HIS

GLN

GLY

LYS

ALA

GLY

ILE

LYS

ILE

2

LEU

GLU

PRO

GLU

GLY

SER

GLU

VAL

SER

3

VAL

THR

VAL

ASP

MET

VAL

THR

LEU

4

ALA

THR

GLU

ALA

LEU

ALA

THR

ALA

VAL

5

THR

GLN

LEU

THR

VAL

PRO

VAL

GLY

ALA

6

ALA

VAL

THR

ALA

ASP

GLU

THR

GLU

VAL

LEU

7

LYS

ALA

GLU

ILE

SER

LYS

ALA

VAL

LYS

GLN

8

VAL

GLN

GLU

ASP

PRO

ASN

THR

HIS

ILE

9

LEU

TYR

HIS

Samples:

sample_1: Myc interacting domain 2 (Mid2), [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 20 mM; sodium azide 0,05%

sample_conditions_1: ionic strength: 40 mM; pH: 7.4; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v3.2.12 - chemical shift assignment, data analysis, peak picking

TOPSPIN v3.6.3 - collection, processing

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks