BMRB Entry 53024

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53024

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
Assignment of the L317Q Delta Prime subunit of the clamp loader
Deposition date:
2025-04-12
Original release date:
2025-11-14
Authors:
Mahdi, Sam; Korzhnev, Dmitry
Citation:

Citation: Mahdi, Sam; Semenova, Irina; Bezsonova, Irina; Beuning, Penny; Korzhnev, Dmitry. "Divide-and-conquer strategy for NMR studies of the E. coli g-clamp loader complex"  J. Biomol. NMR ., .-. (2025).
PubMed: 40694188

Assembly members:

Assembly members:
entity_1, polymer, 334 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MRWYPWLRPDFEKLVASYQA GRGHHALLIQALPGMGDDAL IYALSRYLLCQQPQGHKSCG HCRGCQLMQAGTHPDYYTLA PEKGKNTLGVDAVREVTEKL NEHARLGGAKVVWVTDAALL TDAAANALLKTLEEPPAETW FFLATREPERLLATLRSRCR LHYLAPPPEQYAVTWLSREV TMSQDALLAALRLSAGSPGA ALALFQGDNWQARETLCQAL AYSVPSGDWYSLLAALNHEQ APARLHWLATLLMDALKRHH GAAQVTNVDVPGLVAELANH LSPSRLQAILGDVCHIREQL MSVTGINRELLITDLLQRIE HYLQPGVVLPVPHL

Data sets:
Data typeCount
13C chemical shifts270
15N chemical shifts500
1H chemical shifts1310

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1The L317Q Delta Prime subunit of the clamp loader1

Entities:

Entity 1, The L317Q Delta Prime subunit of the clamp loader 334 residues - Formula weight is not available

1   METARGTRPTYRPROTRPLEUARGPROASP
2   PHEGLULYSLEUVALALASERTYRGLNALA
3   GLYARGGLYHISHISALALEULEUILEGLN
4   ALALEUPROGLYMETGLYASPASPALALEU
5   ILETYRALALEUSERARGTYRLEULEUCYS
6   GLNGLNPROGLNGLYHISLYSSERCYSGLY
7   HISCYSARGGLYCYSGLNLEUMETGLNALA
8   GLYTHRHISPROASPTYRTYRTHRLEUALA
9   PROGLULYSGLYLYSASNTHRLEUGLYVAL
10   ASPALAVALARGGLUVALTHRGLULYSLEU
11   ASNGLUHISALAARGLEUGLYGLYALALYS
12   VALVALTRPVALTHRASPALAALALEULEU
13   THRASPALAALAALAASNALALEULEULYS
14   THRLEUGLUGLUPROPROALAGLUTHRTRP
15   PHEPHELEUALATHRARGGLUPROGLUARG
16   LEULEUALATHRLEUARGSERARGCYSARG
17   LEUHISTYRLEUALAPROPROPROGLUGLN
18   TYRALAVALTHRTRPLEUSERARGGLUVAL
19   THRMETSERGLNASPALALEULEUALAALA
20   LEUARGLEUSERALAGLYSERPROGLYALA
21   ALALEUALALEUPHEGLNGLYASPASNTRP
22   GLNALAARGGLUTHRLEUCYSGLNALALEU
23   ALATYRSERVALPROSERGLYASPTRPTYR
24   SERLEULEUALAALALEUASNHISGLUGLN
25   ALAPROALAARGLEUHISTRPLEUALATHR
26   LEULEUMETASPALALEULYSARGHISHIS
27   GLYALAALAGLNVALTHRASNVALASPVAL
28   PROGLYLEUVALALAGLULEUALAASNHIS
29   LEUSERPROSERARGLEUGLNALAILELEU
30   GLYASPVALCYSHISILEARGGLUGLNLEU
31   METSERVALTHRGLYILEASNARGGLULEU
32   LEUILETHRASPLEULEUGLNARGILEGLU
33   HISTYRLEUGLNPROGLYVALVALLEUPRO
34   VALPROHISLEU

Samples:

sample_1: The L317Q Delta Prime subunit of the clamp loader, [U-13C; U-15N; U-2H; 99% 1HD-Ile,Leu; 99% 1HG-Val], 0.5 mM; PO4 50 mM; B-merc 10 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESY TROSYsample_1isotropicsample_conditions_1
3D 13CH3 HCH NOESY-HMQCsample_1isotropicsample_conditions_1
4D 13CH3 HMQC-NOESY_HMQCsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY v1.4 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks