BMRB Entry 53023

Name: NMR data of MeV Vc 221-299
Published: 2025-09-03

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53023

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR data of MeV Vc 221-299

Deposition date:

2025-04-11

Original release date:

2025-09-03

Authors:

Morita, Kaho; Goda, Nanaka; Kimoto, Madoka; Inoue, Satomi; Yabuno, Nana; Sugiyama, Aoi; Kumeta, Hiroyuki; Ose, Toyoyuki

Citation:

Citation: Morita, Kaho; Goda, Nanaka; Kimoto, Madoka; Inoue, Satomi; Yabuno, Nana; Sugiyama, Aoi; Kumeta, Hiroyuki; Ose, Toyoyuki. "Mechanistic analysis of STAT2 targeting by measles virus V protein" .

Assembly members:

Assembly members:
entity_1, polymer, 81 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Measles morbillivirus Taxonomy ID: 11234 Superkingdom: Viruses Kingdom: not available Genus/species: Morbillivirus hominis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPASTSETPIKKGHRREIGL IWNGDRVFIDRWCNPMCSKV TLGTIRARCTCGECPRVCEQ CRTDTGVDTRIWYHNLPEIP E

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	232
15N chemical shifts	83
1H chemical shifts	541

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MeV Vc	1
2	Zn ion, 1	2
3	Zn ion, 2	2

Entities:

Entity 1, MeV Vc 81 residues - Formula weight is not available

1

GLY

PRO

ALA

SER

THR

SER

GLU

THR

PRO

ILE

2

LYS

GLY

HIS

ARG

GLU

ILE

GLY

LEU

3

ILE

TRP

ASN

GLY

ASP

ARG

VAL

PHE

ILE

ASP

4

ARG

TRP

CYS

ASN

PRO

MET

CYS

SER

LYS

VAL

5

THR

LEU

GLY

THR

ILE

ARG

ALA

ARG

CYS

THR

6

CYS

GLY

GLU

CYS

PRO

ARG

VAL

CYS

GLU

GLN

7

CYS

ARG

THR

ASP

THR

GLY

VAL

ASP

THR

ARG

8

ILE

TRP

TYR

HIS

ASN

LEU

PRO

GLU

ILE

PRO

9

GLU

Entity 2, Zn ion, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: MeV Vc, [U-99% 13C; U-99% 15N], 130 uM; HEPES 10 mM; TCEP 1 mM; MPD 5%; D2O, [U-99% 2H], 3.8%

sample_conditions_1: ionic strength: 11 mM; pH: 6.8; pressure: 1 atm; temperature: 293.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HMQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 15N-separated NOESY	sample_1	isotropic	sample_conditions_1
3D 13C-separated NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.1.4 - collection, processing

NMRPipe v8.2 - processing

SPARKY v3.113 - chemical shift assignment, peak picking

TALOS+ v8.2 - data analysis

CYANA v2.1 - structure solution

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks