BMRB Entry 53015

Name: Backbone resonance assignment of FoxP1_m7 (RBR+FKH)
Published: 2025-04-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53015

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignment of FoxP1_m7 (RBR+FKH)

Deposition date:

2025-04-09

Original release date:

2025-04-15

Authors:

Viennet, Thibault; Leng, Fangwei; Hur, Sun; Arthanari, Haribabu

Citation:

Citation: Leng, Fangwei; Clark, Ryan; Zhang, Wenxiang; Viennet, Thibault; Xi, Wang; Wang, Cuidie; Arthanari, Haribabu; Hur, Sun. "FoxP3 forms a head-to-head dimer in vivo and stabilizes its multimerization on adjacent microsatellites" Cell Reports ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 150 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET50

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPGHTPTTPTAPLTPVTQGP SVITTTSMHTVGPIRRRYSD KYNVPISSADIAQNQEFYKN AEVRPPFTYASLIRQAILES PEKQLTLNEIYNWFTRMFAY FRRNAATWKNAVRHNLSLHK CFVRVENVKGAVWTVDEVEF QKRRPQKISG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
- assigned_chemical_shifts_2

Data type	Count
13C chemical shifts	593
15N chemical shifts	184
1H chemical shifts	184

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FoxP1_m7	1

Entities:

Entity 1, FoxP1_m7 150 residues - Formula weight is not available

aa 433-579 in murine FoxP1

1	GLY	PRO	GLY	HIS	THR	PRO	THR	THR	PRO	THR
2	ALA	PRO	LEU	THR	PRO	VAL	THR	GLN	GLY	PRO
3	SER	VAL	ILE	THR	THR	THR	SER	MET	HIS	THR
4	VAL	GLY	PRO	ILE	ARG	ARG	ARG	TYR	SER	ASP
5	LYS	TYR	ASN	VAL	PRO	ILE	SER	SER	ALA	ASP
6	ILE	ALA	GLN	ASN	GLN	GLU	PHE	TYR	LYS	ASN
7	ALA	GLU	VAL	ARG	PRO	PRO	PHE	THR	TYR	ALA
8	SER	LEU	ILE	ARG	GLN	ALA	ILE	LEU	GLU	SER
9	PRO	GLU	LYS	GLN	LEU	THR	LEU	ASN	GLU	ILE
10	TYR	ASN	TRP	PHE	THR	ARG	MET	PHE	ALA	TYR
11	PHE	ARG	ARG	ASN	ALA	ALA	THR	TRP	LYS	ASN
12	ALA	VAL	ARG	HIS	ASN	LEU	SER	LEU	HIS	LYS
13	CYS	PHE	VAL	ARG	VAL	GLU	ASN	VAL	LYS	GLY
14	ALA	VAL	TRP	THR	VAL	ASP	GLU	VAL	GLU	PHE
15	GLN	LYS	ARG	ARG	PRO	GLN	LYS	ILE	SER	GLY

Samples:

sample_1: FoxP1_m7, [U-100% 13C; U-100% 15N], 700 uM; D2O 5 v/v; MES 20 mM; sodium chloride 150 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 0.17 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0 - collection

NMRPipe - processing

CcpNMR v2.6 - chemical shift assignment

TALOS-N - data analysis

NMR spectrometers:

Bruker AVANCE NEO 700 MHz

1	GLY	PRO	GLY	HIS	THR	PRO	THR	THR	PRO	THR
2	ALA	PRO	LEU	THR	PRO	VAL	THR	GLN	GLY	PRO
3	SER	VAL	ILE	THR	THR	THR	SER	MET	HIS	THR
4	VAL	GLY	PRO	ILE	ARG	ARG	ARG	TYR	SER	ASP
5	LYS	TYR	ASN	VAL	PRO	ILE	SER	SER	ALA	ASP
6	ILE	ALA	GLN	ASN	GLN	GLU	PHE	TYR	LYS	ASN
7	ALA	GLU	VAL	ARG	PRO	PRO	PHE	THR	TYR	ALA
8	SER	LEU	ILE	ARG	GLN	ALA	ILE	LEU	GLU	SER
9	PRO	GLU	LYS	GLN	LEU	THR	LEU	ASN	GLU	ILE
10	TYR	ASN	TRP	PHE	THR	ARG	MET	PHE	ALA	TYR
11	PHE	ARG	ARG	ASN	ALA	ALA	THR	TRP	LYS	ASN
12	ALA	VAL	ARG	HIS	ASN	LEU	SER	LEU	HIS	LYS
13	CYS	PHE	VAL	ARG	VAL	GLU	ASN	VAL	LYS	GLY
14	ALA	VAL	TRP	THR	VAL	ASP	GLU	VAL	GLU	PHE
15	GLN	LYS	ARG	ARG	PRO	GLN	LYS	ILE	SER	GLY

1	GLY	PRO	GLY	HIS	THR	PRO	THR	THR	PRO	THR
2	ALA	PRO	LEU	THR	PRO	VAL	THR	GLN	GLY	PRO
3	SER	VAL	ILE	THR	THR	THR	SER	MET	HIS	THR
4	VAL	GLY	PRO	ILE	ARG	ARG	ARG	TYR	SER	ASP
5	LYS	TYR	ASN	VAL	PRO	ILE	SER	SER	ALA	ASP
6	ILE	ALA	GLN	ASN	GLN	GLU	PHE	TYR	LYS	ASN
7	ALA	GLU	VAL	ARG	PRO	PRO	PHE	THR	TYR	ALA
8	SER	LEU	ILE	ARG	GLN	ALA	ILE	LEU	GLU	SER
9	PRO	GLU	LYS	GLN	LEU	THR	LEU	ASN	GLU	ILE
10	TYR	ASN	TRP	PHE	THR	ARG	MET	PHE	ALA	TYR
11	PHE	ARG	ARG	ASN	ALA	ALA	THR	TRP	LYS	ASN
12	ALA	VAL	ARG	HIS	ASN	LEU	SER	LEU	HIS	LYS
13	CYS	PHE	VAL	ARG	VAL	GLU	ASN	VAL	LYS	GLY
14	ALA	VAL	TRP	THR	VAL	ASP	GLU	VAL	GLU	PHE
15	GLN	LYS	ARG	ARG	PRO	GLN	LYS	ILE	SER	GLY