BMRB Entry 53011

Name: Streptococcal Protein G antibody-binding domain C2 - variant C2Ca EP7-G35E
Published: 2025-04-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53011

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Streptococcal Protein G antibody-binding domain C2 - variant C2Ca EP7-G35E

Deposition date:

2025-04-07

Original release date:

2025-04-17

Authors:

Nagy, Tamas Milan; Jonsson, Malin; Hober, Sophia; Wolf-Watz, Magnus

Citation:

Citation: Nagy, Tamas Milan; Jonsson, Malin; Hober, Sophia; Wolf-Watz, Magnus. "Directed evolution of a calcium-dependent protein enabling mild purification of Fab-fragments" .

Assembly members:

Assembly members:
entity_1, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pBad2.2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TTYKLVINGKTLKGETTTEP PDVNNDGFIDFEDAEKVFKQ YANDNGVDGEWTYDDATKTF TVTE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	179
15N chemical shifts	61
1H chemical shifts	61

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EP7	1

Entities:

Entity 1, EP7 64 residues - Formula weight is not available

1

THR

TYR

LYS

LEU

VAL

ILE

ASN

GLY

LYS

2

THR

LEU

LYS

GLY

GLU

THR

GLU

PRO

3

PRO

ASP

VAL

ASN

ASP

GLY

PHE

ILE

ASP

4

PHE

GLU

ASP

ALA

GLU

LYS

VAL

PHE

LYS

GLN

5

TYR

ALA

ASN

ASP

ASN

GLY

VAL

ASP

GLY

GLU

6

TRP

THR

TYR

ASP

ALA

THR

LYS

THR

PHE

7

THR

VAL

THR

GLU

Samples:

sample_1: EP7-G35E, [U-100% 13C; U-100% 15N], 1 mM; CaCl2 3 mM; MES 30 mM

sample_conditions_1: ionic strength: 3 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection, processing

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks