BMRB Entry 53009
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53009
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Meng, Lingshen; Ye, Shangxiang; Pei, Kai; Tang, Chun. "Structural Dynamics of SARS-CoV-2 NSP4 C-terminal Domain and Implications for Viral Processing" J. Mol. Biol. 437, 169372-169372 (2025).
PubMed: 40763845
Assembly members:
entity_1, polymer, 93 residues, Formula weight is not available
Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet-28a
Entity Sequences (FASTA):
entity_1: SFSTFEEAALCTFLLNKEMY
LKLRSDVLLPLTQYNRYLAL
YNKYKYFSGAMDTTSYREAA
CCHLAKALNDFSNSGSDVLY
QPPQTSITSAVLQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 293 |
| 15N chemical shifts | 84 |
| 1H chemical shifts | 320 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | nsp4CTD | 1 |
Entities:
Entity 1, nsp4CTD 93 residues - Formula weight is not available
| 1 | SER | PHE | SER | THR | PHE | GLU | GLU | ALA | ALA | LEU | ||||
| 2 | CYS | THR | PHE | LEU | LEU | ASN | LYS | GLU | MET | TYR | ||||
| 3 | LEU | LYS | LEU | ARG | SER | ASP | VAL | LEU | LEU | PRO | ||||
| 4 | LEU | THR | GLN | TYR | ASN | ARG | TYR | LEU | ALA | LEU | ||||
| 5 | TYR | ASN | LYS | TYR | LYS | TYR | PHE | SER | GLY | ALA | ||||
| 6 | MET | ASP | THR | THR | SER | TYR | ARG | GLU | ALA | ALA | ||||
| 7 | CYS | CYS | HIS | LEU | ALA | LYS | ALA | LEU | ASN | ASP | ||||
| 8 | PHE | SER | ASN | SER | GLY | SER | ASP | VAL | LEU | TYR | ||||
| 9 | GLN | PRO | PRO | GLN | THR | SER | ILE | THR | SER | ALA | ||||
| 10 | VAL | LEU | GLN |
Samples:
sample_1: nsp4CTD, [U-95% 13C; U-90% 15N], 0.7 mM; HEPES 20 mM; sodium chloride 150 mM
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks