Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53008

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: S-nitrosylated PTBP1-RRM2 with Raver1 peptide
Published: 2026-05-21

Title:

S-nitrosylated PTBP1-RRM2 with Raver1 peptide

Deposition date:

2025-04-05

Original release date:

2026-05-21

Authors:

Schindler, Joseph; Hausladen, Alfred; Yang, Jun; Qin, Jun; Stamler, Jonathan

Citation:

Citation: Schindler, Joseph; Seth, Puneet; Hausladen, Alfred; Chan, Ricky; Yang, Jun; Qin, Jun; Qian, Zhaoxia; Seth, Divya; Lundberg, Kathleen; Luna, Joseph; Premont, Richard; Stamler, Jonathan. "Nitric oxide drives proteomic diversity through alternative splicing" .

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, 14160 Da.
entity_2, polymer, 12 residues, 1150 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GMAMAGQSPVLRIIVENLFY PVTLDVLHQIFSKFGTVLKI ITFTKNNQFQALLQYADPVS AQHAKLSLDGQNIYNACCTL RIDFSKLTSLNVKYNNDKSR DYTRPDLPSG
entity_2: PGVSLLGAPPKD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
15N chemical shifts	94
1H chemical shifts	94

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PTBP1-RRM2	1
2	Raver1	2

Entities:

Entity 1, PTBP1-RRM2 110 residues - 14160 Da.

1	GLY	MET	ALA	MET	ALA	GLY	GLN	SER	PRO	VAL
2	LEU	ARG	ILE	ILE	VAL	GLU	ASN	LEU	PHE	TYR
3	PRO	VAL	THR	LEU	ASP	VAL	LEU	HIS	GLN	ILE
4	PHE	SER	LYS	PHE	GLY	THR	VAL	LEU	LYS	ILE
5	ILE	THR	PHE	THR	LYS	ASN	ASN	GLN	PHE	GLN
6	ALA	LEU	LEU	GLN	TYR	ALA	ASP	PRO	VAL	SER
7	ALA	GLN	HIS	ALA	LYS	LEU	SER	LEU	ASP	GLY
8	GLN	ASN	ILE	TYR	ASN	ALA	CYS	CYS	THR	LEU
9	ARG	ILE	ASP	PHE	SER	LYS	LEU	THR	SER	LEU
10	ASN	VAL	LYS	TYR	ASN	ASN	ASP	LYS	SER	ARG
11	ASP	TYR	THR	ARG	PRO	ASP	LEU	PRO	SER	GLY

Entity 2, Raver1 12 residues - 1150 Da.

1

PRO

GLY

VAL

SER

LEU

GLY

ALA

PRO

2

LYS

ASP

Samples:

sample_1: PTBP1-RRM2, [U-15N], 150 uM; Raver1 Interacting Peptide 1500 uM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

POKY - data analysis

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	GLY	MET	ALA	MET	ALA	GLY	GLN	SER	PRO	VAL
2	LEU	ARG	ILE	ILE	VAL	GLU	ASN	LEU	PHE	TYR
3	PRO	VAL	THR	LEU	ASP	VAL	LEU	HIS	GLN	ILE
4	PHE	SER	LYS	PHE	GLY	THR	VAL	LEU	LYS	ILE
5	ILE	THR	PHE	THR	LYS	ASN	ASN	GLN	PHE	GLN
6	ALA	LEU	LEU	GLN	TYR	ALA	ASP	PRO	VAL	SER
7	ALA	GLN	HIS	ALA	LYS	LEU	SER	LEU	ASP	GLY
8	GLN	ASN	ILE	TYR	ASN	ALA	CYS	CYS	THR	LEU
9	ARG	ILE	ASP	PHE	SER	LYS	LEU	THR	SER	LEU
10	ASN	VAL	LYS	TYR	ASN	ASN	ASP	LYS	SER	ARG
11	ASP	TYR	THR	ARG	PRO	ASP	LEU	PRO	SER	GLY

1	GLY	MET	ALA	MET	ALA	GLY	GLN	SER	PRO	VAL
2	LEU	ARG	ILE	ILE	VAL	GLU	ASN	LEU	PHE	TYR
3	PRO	VAL	THR	LEU	ASP	VAL	LEU	HIS	GLN	ILE
4	PHE	SER	LYS	PHE	GLY	THR	VAL	LEU	LYS	ILE
5	ILE	THR	PHE	THR	LYS	ASN	ASN	GLN	PHE	GLN
6	ALA	LEU	LEU	GLN	TYR	ALA	ASP	PRO	VAL	SER
7	ALA	GLN	HIS	ALA	LYS	LEU	SER	LEU	ASP	GLY
8	GLN	ASN	ILE	TYR	ASN	ALA	CYS	CYS	THR	LEU
9	ARG	ILE	ASP	PHE	SER	LYS	LEU	THR	SER	LEU
10	ASN	VAL	LYS	TYR	ASN	ASN	ASP	LYS	SER	ARG
11	ASP	TYR	THR	ARG	PRO	ASP	LEU	PRO	SER	GLY

BMRB Entry 53008