BMRB Entry 53000

Name: NMR backbone assignment of the WRC domain from A. thaliana GRF3
Published: 2025-05-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53000

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR backbone assignment of the WRC domain from A. thaliana GRF3

Deposition date:

2025-04-03

Original release date:

2025-05-23

Authors:

Biglione, Franco; Palatnik, Javier; Nahuel, Gonzalez Schain; Rodolfo, Rasia

Citation:

Citation: Biglione, Franco; Gonzalez Schain, Javier; Palatnik, Nahuel; Rasia, Rodolfo. "The WRC domain of GRF transcription factors: Structure and DNA recognition" Protein Sci. 34, e70172-e70172 (2025).
PubMed: 40437964

Assembly members:

Assembly members:
entity_1, polymer, . residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGRAAMDPEPGRCRRTDGKK WRCSRDVFAGHKYCERHMHR GRNRSRKPVETPTTVN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	128
15N chemical shifts	42
1H chemical shifts	213

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WRC	1
2	Zn	2

Entities:

Entity 1, WRC - Formula weight is not available

1

GLY

ARG

ALA

MET

ASP

PRO

GLU

PRO

2

GLY

ARG

CYS

ARG

THR

ASP

GLY

LYS

3

TRP

ARG

CYS

SER

ARG

ASP

VAL

PHE

ALA

GLY

4

HIS

LYS

TYR

CYS

GLU

ARG

HIS

MET

HIS

ARG

5

GLY

ARG

ASN

ARG

SER

ARG

LYS

PRO

VAL

GLU

6

THR

PRO

THR

VAL

ASN

Entity 2, Zn - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: WRC, [U-100% 13C; U-100% 15N], 600 uM; Zn 600 uM; TCEP 5 mM; HEPES 20 mM; sodium chloride 50 mM

sample_2: WRC, [U-100% 15N], 600 uM; Zn 600 uM; TCEP 5 mM; HEPES 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_2	isotropic	sample_conditions_1

Software:

CcpNMR - chemical shift assignment

TOPSPIN - collection

NMRPipe - processing

NMR spectrometers:

Bruker AVANCE III 700 MHz