BMRB Entry 5300

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5300

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Resonance Assignments for the 20.8 KDa C.elegans gene product ORF C32E8.3. Northeast Structural Genomics Consortium Target WR33.

Deposition date:

2002-02-22

Original release date:

2002-06-27

Authors:

Monleon, Daniel; YiWen, Chiang; Aramini, James; Swapna, G.V.T.; Kim, Seho; Szyperski, Thomas; Montelione, Gaetano

Citation:

Citation: Monleon, Daniel; YiWen, Chiang; Aramini, James; Swapna, G.V.T.; Macapagal, Daphne; Gunsalus, Kristin; Kim, Seho; Szyperski, Thomas; Montelione, Gaetano. "Letter to the Editor: Backbone 1H, 15N and 13C assignments for the 21 kDa Caenorhabditis elegans homologue of 'brain-specific' protein" J. Biomol. NMR 28, 91-92 (2004).

Assembly members:

Assembly members:
ORF C32E8.3, polymer, 190 residues, 20800 Da.

Natural source:

Natural source: Common Name: C.elegans. Taxonomy ID: 6239 Superkingdom: Eubacteria Kingdom: not available Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ORF C32E8.3: MGHHHHHHSHMAAAAGFNWD DADVKKRWDAFTKFGAATAT EMTGKNFDKWLKDAGVLDNK AITGTMTGIAFSKVTGPKKK ATFDETKKVLAFVAEDRARQ SKKPIQDELDAITEKLAKLE APSVGGAAKANAAGVYSRLT DHTKYTGAHKERFDAEGKGK GKSGRADTTENTGYVGAYKN KDSYDKTHGK

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	331
13C chemical shifts	496
15N chemical shifts	175

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C.elegans ORF C32E8.3	1

Entities:

Entity 1, C.elegans ORF C32E8.3 190 residues - 20800 Da.

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	SER	HIS
2	MET	ALA	ALA	ALA	ALA	GLY	PHE	ASN	TRP	ASP
3	ASP	ALA	ASP	VAL	LYS	LYS	ARG	TRP	ASP	ALA
4	PHE	THR	LYS	PHE	GLY	ALA	ALA	THR	ALA	THR
5	GLU	MET	THR	GLY	LYS	ASN	PHE	ASP	LYS	TRP
6	LEU	LYS	ASP	ALA	GLY	VAL	LEU	ASP	ASN	LYS
7	ALA	ILE	THR	GLY	THR	MET	THR	GLY	ILE	ALA
8	PHE	SER	LYS	VAL	THR	GLY	PRO	LYS	LYS	LYS
9	ALA	THR	PHE	ASP	GLU	THR	LYS	LYS	VAL	LEU
10	ALA	PHE	VAL	ALA	GLU	ASP	ARG	ALA	ARG	GLN
11	SER	LYS	LYS	PRO	ILE	GLN	ASP	GLU	LEU	ASP
12	ALA	ILE	THR	GLU	LYS	LEU	ALA	LYS	LEU	GLU
13	ALA	PRO	SER	VAL	GLY	GLY	ALA	ALA	LYS	ALA
14	ASN	ALA	ALA	GLY	VAL	TYR	SER	ARG	LEU	THR
15	ASP	HIS	THR	LYS	TYR	THR	GLY	ALA	HIS	LYS
16	GLU	ARG	PHE	ASP	ALA	GLU	GLY	LYS	GLY	LYS
17	GLY	LYS	SER	GLY	ARG	ALA	ASP	THR	THR	GLU
18	ASN	THR	GLY	TYR	VAL	GLY	ALA	TYR	LYS	ASN
19	LYS	ASP	SER	TYR	ASP	LYS	THR	HIS	GLY	LYS

Samples:

sample_1: ORF C32E8.3, [U-13C; U-15N], 1.4 mM

sample_2: ORF C32E8.3, [U-13C; U-15N], 0.5 mM

sample_3: ORF C32E8.3, [U-13C; U-15N], 0.5 mM; DMPC:DHCP:CTAB(30:10:1) 4.5%

condition_1: pH: 6.50; temperature: 298 K

condition_2: pH: 6.50; temperature: 298 K

condition_3: pH: 6.50; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
HNCO	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
(HA)CA(CO)NH	not available	not available	not available
HA(CA)(CO)NH	not available	not available	not available
(HA)CANH	not available	not available	not available
HA(CA)NH	not available	not available	not available
(H)CC(CO)NH-TOCSY	not available	not available	not available
13C-edited NOESY	not available	not available	not available
15N-edited NOESY	not available	not available	not available
RD-HNCA,HA	not available	not available	not available
RD-HA,CA(CO)NH	not available	not available	not available
RDC-JHC COUPLED (HA)CANH	not available	not available	not available

Software:

NMRPipe v2000 - data processing

SPARKY v3.91 - peak picking

AUTOASSIGN v1.8.0 - automated analysis of backbone 1H, 13C and 15N assignments

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz
Varian INOVA 750 MHz

PDB	1PUL
EMBL	CCD66401
REF	NP_491219
SP	P91127
AlphaFold	P91127

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	SER	HIS
2	MET	ALA	ALA	ALA	ALA	GLY	PHE	ASN	TRP	ASP
3	ASP	ALA	ASP	VAL	LYS	LYS	ARG	TRP	ASP	ALA
4	PHE	THR	LYS	PHE	GLY	ALA	ALA	THR	ALA	THR
5	GLU	MET	THR	GLY	LYS	ASN	PHE	ASP	LYS	TRP
6	LEU	LYS	ASP	ALA	GLY	VAL	LEU	ASP	ASN	LYS
7	ALA	ILE	THR	GLY	THR	MET	THR	GLY	ILE	ALA
8	PHE	SER	LYS	VAL	THR	GLY	PRO	LYS	LYS	LYS
9	ALA	THR	PHE	ASP	GLU	THR	LYS	LYS	VAL	LEU
10	ALA	PHE	VAL	ALA	GLU	ASP	ARG	ALA	ARG	GLN
11	SER	LYS	LYS	PRO	ILE	GLN	ASP	GLU	LEU	ASP
12	ALA	ILE	THR	GLU	LYS	LEU	ALA	LYS	LEU	GLU
13	ALA	PRO	SER	VAL	GLY	GLY	ALA	ALA	LYS	ALA
14	ASN	ALA	ALA	GLY	VAL	TYR	SER	ARG	LEU	THR
15	ASP	HIS	THR	LYS	TYR	THR	GLY	ALA	HIS	LYS
16	GLU	ARG	PHE	ASP	ALA	GLU	GLY	LYS	GLY	LYS
17	GLY	LYS	SER	GLY	ARG	ALA	ASP	THR	THR	GLU
18	ASN	THR	GLY	TYR	VAL	GLY	ALA	TYR	LYS	ASN
19	LYS	ASP	SER	TYR	ASP	LYS	THR	HIS	GLY	LYS

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	SER	HIS
2	MET	ALA	ALA	ALA	ALA	GLY	PHE	ASN	TRP	ASP
3	ASP	ALA	ASP	VAL	LYS	LYS	ARG	TRP	ASP	ALA
4	PHE	THR	LYS	PHE	GLY	ALA	ALA	THR	ALA	THR
5	GLU	MET	THR	GLY	LYS	ASN	PHE	ASP	LYS	TRP
6	LEU	LYS	ASP	ALA	GLY	VAL	LEU	ASP	ASN	LYS
7	ALA	ILE	THR	GLY	THR	MET	THR	GLY	ILE	ALA
8	PHE	SER	LYS	VAL	THR	GLY	PRO	LYS	LYS	LYS
9	ALA	THR	PHE	ASP	GLU	THR	LYS	LYS	VAL	LEU
10	ALA	PHE	VAL	ALA	GLU	ASP	ARG	ALA	ARG	GLN
11	SER	LYS	LYS	PRO	ILE	GLN	ASP	GLU	LEU	ASP
12	ALA	ILE	THR	GLU	LYS	LEU	ALA	LYS	LEU	GLU
13	ALA	PRO	SER	VAL	GLY	GLY	ALA	ALA	LYS	ALA
14	ASN	ALA	ALA	GLY	VAL	TYR	SER	ARG	LEU	THR
15	ASP	HIS	THR	LYS	TYR	THR	GLY	ALA	HIS	LYS
16	GLU	ARG	PHE	ASP	ALA	GLU	GLY	LYS	GLY	LYS
17	GLY	LYS	SER	GLY	ARG	ALA	ASP	THR	THR	GLU
18	ASN	THR	GLY	TYR	VAL	GLY	ALA	TYR	LYS	ASN
19	LYS	ASP	SER	TYR	ASP	LYS	THR	HIS	GLY	LYS