BMRB Entry 5300

Title:
Backbone 1H, 13C, and 15N Resonance Assignments for the 20.8 KDa C.elegans gene product ORF C32E8.3. Northeast Structural Genomics Consortium Target WR33.
Deposition date:
2002-02-22
Original release date:
2002-06-27
Authors:
Monleon, Daniel; YiWen, Chiang; Aramini, James; Swapna, G.V.T.; Kim, Seho; Szyperski, Thomas; Montelione, Gaetano
Citation:

Citation: Monleon, Daniel; YiWen, Chiang; Aramini, James; Swapna, G.V.T.; Macapagal, Daphne; Gunsalus, Kristin; Kim, Seho; Szyperski, Thomas; Montelione, Gaetano. "Letter to the Editor: Backbone 1H, 15N and 13C assignments for the 21 kDa Caenorhabditis elegans homologue of 'brain-specific' protein"  J. Biomol. NMR 28, 91-92 (2004).

Assembly members:

Assembly members:
ORF C32E8.3, polymer, 190 residues, 20800 Da.

Natural source:

Natural source:   Common Name: C.elegans.   Taxonomy ID: 6239   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
1H chemical shifts331
13C chemical shifts496
15N chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C.elegans ORF C32E8.31

Entities:

Entity 1, C.elegans ORF C32E8.3 190 residues - 20800 Da.

1   METGLYHISHISHISHISHISHISSERHIS
2   METALAALAALAALAGLYPHEASNTRPASP
3   ASPALAASPVALLYSLYSARGTRPASPALA
4   PHETHRLYSPHEGLYALAALATHRALATHR
5   GLUMETTHRGLYLYSASNPHEASPLYSTRP
6   LEULYSASPALAGLYVALLEUASPASNLYS
7   ALAILETHRGLYTHRMETTHRGLYILEALA
8   PHESERLYSVALTHRGLYPROLYSLYSLYS
9   ALATHRPHEASPGLUTHRLYSLYSVALLEU
10   ALAPHEVALALAGLUASPARGALAARGGLN
11   SERLYSLYSPROILEGLNASPGLULEUASP
12   ALAILETHRGLULYSLEUALALYSLEUGLU
13   ALAPROSERVALGLYGLYALAALALYSALA
14   ASNALAALAGLYVALTYRSERARGLEUTHR
15   ASPHISTHRLYSTYRTHRGLYALAHISLYS
16   GLUARGPHEASPALAGLUGLYLYSGLYLYS
17   GLYLYSSERGLYARGALAASPTHRTHRGLU
18   ASNTHRGLYTYRVALGLYALATYRLYSASN
19   LYSASPSERTYRASPLYSTHRHISGLYLYS

Samples:

sample_1: ORF C32E8.3, [U-13C; U-15N], 1.4 mM

sample_2: ORF C32E8.3, [U-13C; U-15N], 0.5 mM

sample_3: ORF C32E8.3, [U-13C; U-15N], 0.5 mM; DMPC:DHCP:CTAB(30:10:1) 4.5%

condition_1: pH: 6.50; temperature: 298 K

condition_2: pH: 6.50; temperature: 298 K

condition_3: pH: 6.50; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
(HA)CA(CO)NHnot availablenot availablenot available
HA(CA)(CO)NHnot availablenot availablenot available
(HA)CANHnot availablenot availablenot available
HA(CA)NHnot availablenot availablenot available
(H)CC(CO)NH-TOCSYnot availablenot availablenot available
13C-edited NOESYnot availablenot availablenot available
15N-edited NOESYnot availablenot availablenot available
RD-HNCA,HAnot availablenot availablenot available
RD-HA,CA(CO)NHnot availablenot availablenot available
RDC-JHC COUPLED (HA)CANHnot availablenot availablenot available

Software:

NMRPipe v2000 - data processing

SPARKY v3.91 - peak picking

AUTOASSIGN v1.8.0 - automated analysis of backbone 1H, 13C and 15N assignments

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Related Database Links:

PDB
EMBL CCD66401
REF NP_491219
SP P91127
AlphaFold P91127

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks