BMRB Entry 52982

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52982

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Title:
Sequence-Specific Backbone assignment of the cAMP-dependent protein kinase type II-alpha regulatory subunit disordered region (aa 44-103)
Deposition date:
2025-03-27
Original release date:
2026-01-23
Authors:
Parikh, Tvesha; Page, Rebecca; Peti, Wolfgang
Citation:

Citation: Shirakawa, Karina; Parikh, Tvesha; Machado, Luciana; Poimenidou, G.; Nguyen, Hieu; Dell'Acqua, M.; Kettenbach, Arminja; Page, Rebecca; Peti, Wolfgang. "The clinical missense variant E282K in PPP3CA/calcineurin shifts substrate dephosphorylation by altering active site recruitment"  Nat. Commun. ., .-. (2026).

Assembly members:

Assembly members:
entity_1, polymer, 61 residues, 6489.17 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTHMT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MRAPASVLPAATPRQALGHP PPEPGPDRVADAKGDAESEE DEDLEVPVPSRFNRRVSVCA E

Data sets:
Data typeCount
13C chemical shifts150
15N chemical shifts51
1H chemical shifts51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RII1

Entities:

Entity 1, RII 61 residues - 6489.17 Da.

RII alpha, residues 44-103, RII starting with RAPAS

1   METARGALAPROALASERVALLEUPROALA
2   ALATHRPROARGGLNALALEUGLYHISPRO
3   PROPROGLUPROGLYPROASPARGVALALA
4   ASPALALYSGLYASPALAGLUSERGLUGLU
5   ASPGLUASPLEUGLUVALPROVALPROSER
6   ARGPHEASNARGARGVALSERVALCYSALA
7   GLU

Samples:

sample_1: RII, [U-99% 13C; U-99% 15N], 600 uM; HEPES 10 mM; NaCl 50 mM; TCEP 0.5 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

CARA v1.9 - chemical shift assignment

TOPSPIN v4.1.3 - collection, processing

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks