BMRB Entry 52971

Name: Stm1-IDR 226-273
Published: 2025-04-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52971

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Stm1-IDR 226-273

Deposition date:

2025-03-17

Original release date:

2025-04-22

Authors:

Gossert, Alvar

Citation:

Citation: Bianco, Eliana; Bonassera, Martina; Uliana, Federico; Tilma, Janny; Winkler, Martin; Zencir, Sevil; Albert, Benjamin; Oborska-Oplova, Michaela; Dechant, Reinhard; Hugener, Jannik; Gossert, Alvar; Panse, Vikram; Pilhofer, Martin; Kimmig, Philipp; Peter, Matthias. "Stm1 regulates Ifh1 activity revealing crosstalk between ribosome biogenesis and ribosome dormancy" Mol. Cell ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 47 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NNSRNNFNNRRGGRGARKGN NTANATNSANTVQKNRNIDV SNLPSLA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	124
15N chemical shifts	45
1H chemical shifts	45

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Stm1 226-273	1

Entities:

Entity 1, Stm1 226-273 47 residues - Formula weight is not available

1

ASN

SER

ARG

ASN

PHE

ASN

ARG

2

ARG

GLY

ARG

GLY

ALA

ARG

LYS

GLY

ASN

3

ASN

THR

ALA

ASN

ALA

THR

ASN

SER

ALA

ASN

4

THR

VAL

GLN

LYS

ASN

ARG

ASN

ILE

ASP

VAL

5

SER

ASN

LEU

PRO

SER

LEU

ALA

Samples:

sample_1: Stm1 226-273, [U-98% 13C; U-98% 15N], 0.4 ± 0.1 mM; MMT 40 ± 2 mM; D-techalose 25 ± 2 mM; DTT 1 ± 0.1 mM; DSS 0.011 ± 0.001 mM

sample_conditions_1: ionic strength: 65 mM; pH: 6.1; pressure: 1 atm; temperature: 298.1 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0.9 - collection, processing

CARA v1.9.1.8a2 - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks