BMRB Entry 52970

Name: Chemical shift assignments for Bora 1-120
Published: 2026-01-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52970

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shift assignments for Bora 1-120

Deposition date:

2025-03-14

Original release date:

2026-01-05

Authors:

Miles, Jennifer; Batchelor, Matthew; Walko, Martin; Gunning, Vanda; Wilson, Andrew; Wright, Megan; Bayliss, Richard

Citation:

Citation: Miles, Jennifer; Batchelor, Matthew; Walko, Martin; Gunning, Vanda; Wilson, Andrew; Wright, Megan; Bayliss, Richard. "Bora Bridges Aurora-A Activation and Substrate Recognition of PLK1" EMBO Rep. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petSUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSMGDVKESKMQITPETPGR IPVLNPFESPSDYSNLHEQT LASPSVFKSTKLPTPGKFRW SIDQLAVINPVEIDPEDIHR QALYLSHSRIDKDVEDKRQK AIEEFFTKDVIVPSPWTDHE GK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	360
15N chemical shifts	108
1H chemical shifts	427

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Bora 1-120	1

Entities:

Entity 1, Bora 1-120 122 residues - Formula weight is not available

1

GLY

SER

MET

GLY

ASP

VAL

LYS

GLU

SER

LYS

2

MET

GLN

ILE

THR

PRO

GLU

THR

PRO

GLY

ARG

3

ILE

PRO

VAL

LEU

ASN

PRO

PHE

GLU

SER

PRO

4

SER

ASP

TYR

SER

ASN

LEU

HIS

GLU

GLN

THR

5

LEU

ALA

SER

PRO

SER

VAL

PHE

LYS

SER

THR

6

LYS

LEU

PRO

THR

PRO

GLY

LYS

PHE

ARG

TRP

7

SER

ILE

ASP

GLN

LEU

ALA

VAL

ILE

ASN

PRO

8

VAL

GLU

ILE

ASP

PRO

GLU

ASP

ILE

HIS

ARG

9

GLN

ALA

LEU

TYR

LEU

SER

HIS

SER

ARG

ILE

10

ASP

LYS

ASP

VAL

GLU

ASP

LYS

ARG

GLN

LYS

11

ALA

ILE

GLU

PHE

THR

LYS

ASP

VAL

12

ILE

VAL

PRO

SER

PRO

TRP

THR

ASP

HIS

GLU

13

GLY

LYS

Samples:

sample_1: Bora 1-120, [U-99% 13C; U-99% 15N], 310 uM; (K/H)3PO4 20 mM; NaCl 150 mM; glycerol 2.5%

sample_conditions_1: ionic strength: 0.19 M; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR vAnalysis V2.5 - chemical shift assignment

NMR spectrometers:

Oxford/Bruker AVANCE III 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks