BMRB Entry 5296

Title:
1H Resonance Assignments at 310K and pH 4.90 for E.coli recombinant Brazzein variant brazzein-ins(R18a-I18b), an Non-Sweet variant of Brazzein
Deposition date:
2002-01-18
Original release date:
2005-09-22
Authors:
Zhao, Qin; Song, Jikui; Markley, John
Citation:

Citation: Zhao, Qin; Song, Jikui; Jin, Zheyuan; Danilova, Vicktoria; Hellekant, Goran; Markley, John. "Probing the sweet determinants of brazzein: Wild-type brazzein and a tasteless variant, brazzein-ins(R(18a)-I(18b)), exhibit different pH-dependent NMR chemical shifts"  Biochem. Biophys. Res. Commun. 335, 256-263 (2005).

Assembly members:

Assembly members:
brazzein-ins(R18a-I18b), polymer, 55 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Joublie fruit   Taxonomy ID: 43545   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Pentadiplandra brazzeana

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
brazzein-ins(R18a-I18b): DKCKKVYENYPVSKCQLRIA NQCNYDCKLDKHARSGECFY DEKRNLQCICDYCEY

Data sets:
Data typeCount
1H chemical shifts255

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1brazzein-ins-(R18a-I18b)1

Entities:

Entity 1, brazzein-ins-(R18a-I18b) 55 residues - Formula weight is not available

1   ASPLYSCYSLYSLYSVALTYRGLUASNTYR
2   PROVALSERLYSCYSGLNLEUARGILEALA
3   ASNGLNCYSASNTYRASPCYSLYSLEUASP
4   LYSHISALAARGSERGLYGLUCYSPHETYR
5   ASPGLULYSARGASNLEUGLNCYSILECYS
6   ASPTYRCYSGLUTYR

Samples:

sample_1: brazzein-ins(R18a-I18b) 1 mM; KCl 100 mM; DSS 0.1 mM

conditions_set_1: ionic strength: 100 mM; pH: 4.90; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
TOCSYsample_1not availableconditions_set_1

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz