BMRB Entry 52955

Name: Assembly and functional mechanisms of the Hsp70-Hsp40 chaperone machinery
Published: 2025-10-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52955

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assembly and functional mechanisms of the Hsp70-Hsp40 chaperone machinery

Deposition date:

2025-03-09

Original release date:

2025-10-20

Authors:

Jiang, Yajun; Ibrahim, Ziad; Clay, Mary; Xia, Youlin

Citation:

Citation: Jiang, Yajun; Ibrahim, Ziad; Xia, Youlin; Clay, Mary; Myasnikov, Alexander; Immadisetty, Kalyan; Xia, Zhilian; Tang, Liang; Rossi, Paolo; Ganguly, Pritha; Liu, Jiangshu; Miller, Darcie; Che, Meixia; Palacios, Santiago; Kramer, Gunter; Bukau, Bernd; Kalodimos, Charalampos. "Mechanisms of assembly and function of the Hsp70-Hsp40 chaperone machinery" Mol. Cell ., .-. (2025).
PubMed: 41092901

Assembly members:

Assembly members:
entity_1, polymer, 280 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thermus thermophilus Taxonomy ID: 274 Superkingdom: Bacteria Kingdom: not available Genus/species: Thermus thermophilus

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAAKKDYYAILGVPRNATQE EIKRAYKRLARQYHPDVNKS PEAEEKFKEINEAYAVLSDP EKRRIYDTYGTTEAPPPPPP GGYDFSGFDVEDFSEFFQEL FGPGLFGGFGRRSRKGRDLR AELPLTLEEAFHGGERVVEV AGRRVSVRIPPGVREGSVIR VPGMGGQGNPPGDLLLVVRL LPHPVFRLEGQDLYATLDVP APIAVVGGKVRAMTLEGPVE VAVPPRTQAGRKLRLKGKGF PGPAGRGDLYLEVRITIPER LTPEEEALWKKLAEAYYARA

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	111
1H chemical shifts	333

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DnaJ	1

Entities:

Entity 1, DnaJ 280 residues - Formula weight is not available

1	MET	ALA	ALA	LYS	LYS	ASP	TYR	TYR	ALA	ILE
2	LEU	GLY	VAL	PRO	ARG	ASN	ALA	THR	GLN	GLU
3	GLU	ILE	LYS	ARG	ALA	TYR	LYS	ARG	LEU	ALA
4	ARG	GLN	TYR	HIS	PRO	ASP	VAL	ASN	LYS	SER
5	PRO	GLU	ALA	GLU	GLU	LYS	PHE	LYS	GLU	ILE
6	ASN	GLU	ALA	TYR	ALA	VAL	LEU	SER	ASP	PRO
7	GLU	LYS	ARG	ARG	ILE	TYR	ASP	THR	TYR	GLY
8	THR	THR	GLU	ALA	PRO	PRO	PRO	PRO	PRO	PRO
9	GLY	GLY	TYR	ASP	PHE	SER	GLY	PHE	ASP	VAL
10	GLU	ASP	PHE	SER	GLU	PHE	PHE	GLN	GLU	LEU
11	PHE	GLY	PRO	GLY	LEU	PHE	GLY	GLY	PHE	GLY
12	ARG	ARG	SER	ARG	LYS	GLY	ARG	ASP	LEU	ARG
13	ALA	GLU	LEU	PRO	LEU	THR	LEU	GLU	GLU	ALA
14	PHE	HIS	GLY	GLY	GLU	ARG	VAL	VAL	GLU	VAL
15	ALA	GLY	ARG	ARG	VAL	SER	VAL	ARG	ILE	PRO
16	PRO	GLY	VAL	ARG	GLU	GLY	SER	VAL	ILE	ARG
17	VAL	PRO	GLY	MET	GLY	GLY	GLN	GLY	ASN	PRO
18	PRO	GLY	ASP	LEU	LEU	LEU	VAL	VAL	ARG	LEU
19	LEU	PRO	HIS	PRO	VAL	PHE	ARG	LEU	GLU	GLY
20	GLN	ASP	LEU	TYR	ALA	THR	LEU	ASP	VAL	PRO
21	ALA	PRO	ILE	ALA	VAL	VAL	GLY	GLY	LYS	VAL
22	ARG	ALA	MET	THR	LEU	GLU	GLY	PRO	VAL	GLU
23	VAL	ALA	VAL	PRO	PRO	ARG	THR	GLN	ALA	GLY
24	ARG	LYS	LEU	ARG	LEU	LYS	GLY	LYS	GLY	PHE
25	PRO	GLY	PRO	ALA	GLY	ARG	GLY	ASP	LEU	TYR
26	LEU	GLU	VAL	ARG	ILE	THR	ILE	PRO	GLU	ARG
27	LEU	THR	PRO	GLU	GLU	GLU	ALA	LEU	TRP	LYS
28	LYS	LEU	ALA	GLU	ALA	TYR	TYR	ALA	ARG	ALA

Samples:

sample_1: DnaJ, [U-15N; U-2H; 99% 13CH3-Ile,Leu,Val, Met, Ala, The], 100 uM; H2O 90%; D2O, [U-100% 2H], 10%; potassium phosphate 25 mM; sodium chloride 75 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 315 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HMQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.1.4 - collection

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

1	MET	ALA	ALA	LYS	LYS	ASP	TYR	TYR	ALA	ILE
2	LEU	GLY	VAL	PRO	ARG	ASN	ALA	THR	GLN	GLU
3	GLU	ILE	LYS	ARG	ALA	TYR	LYS	ARG	LEU	ALA
4	ARG	GLN	TYR	HIS	PRO	ASP	VAL	ASN	LYS	SER
5	PRO	GLU	ALA	GLU	GLU	LYS	PHE	LYS	GLU	ILE
6	ASN	GLU	ALA	TYR	ALA	VAL	LEU	SER	ASP	PRO
7	GLU	LYS	ARG	ARG	ILE	TYR	ASP	THR	TYR	GLY
8	THR	THR	GLU	ALA	PRO	PRO	PRO	PRO	PRO	PRO
9	GLY	GLY	TYR	ASP	PHE	SER	GLY	PHE	ASP	VAL
10	GLU	ASP	PHE	SER	GLU	PHE	PHE	GLN	GLU	LEU
11	PHE	GLY	PRO	GLY	LEU	PHE	GLY	GLY	PHE	GLY
12	ARG	ARG	SER	ARG	LYS	GLY	ARG	ASP	LEU	ARG
13	ALA	GLU	LEU	PRO	LEU	THR	LEU	GLU	GLU	ALA
14	PHE	HIS	GLY	GLY	GLU	ARG	VAL	VAL	GLU	VAL
15	ALA	GLY	ARG	ARG	VAL	SER	VAL	ARG	ILE	PRO
16	PRO	GLY	VAL	ARG	GLU	GLY	SER	VAL	ILE	ARG
17	VAL	PRO	GLY	MET	GLY	GLY	GLN	GLY	ASN	PRO
18	PRO	GLY	ASP	LEU	LEU	LEU	VAL	VAL	ARG	LEU
19	LEU	PRO	HIS	PRO	VAL	PHE	ARG	LEU	GLU	GLY
20	GLN	ASP	LEU	TYR	ALA	THR	LEU	ASP	VAL	PRO
21	ALA	PRO	ILE	ALA	VAL	VAL	GLY	GLY	LYS	VAL
22	ARG	ALA	MET	THR	LEU	GLU	GLY	PRO	VAL	GLU
23	VAL	ALA	VAL	PRO	PRO	ARG	THR	GLN	ALA	GLY
24	ARG	LYS	LEU	ARG	LEU	LYS	GLY	LYS	GLY	PHE
25	PRO	GLY	PRO	ALA	GLY	ARG	GLY	ASP	LEU	TYR
26	LEU	GLU	VAL	ARG	ILE	THR	ILE	PRO	GLU	ARG
27	LEU	THR	PRO	GLU	GLU	GLU	ALA	LEU	TRP	LYS
28	LYS	LEU	ALA	GLU	ALA	TYR	TYR	ALA	ARG	ALA

1	MET	ALA	ALA	LYS	LYS	ASP	TYR	TYR	ALA	ILE
2	LEU	GLY	VAL	PRO	ARG	ASN	ALA	THR	GLN	GLU
3	GLU	ILE	LYS	ARG	ALA	TYR	LYS	ARG	LEU	ALA
4	ARG	GLN	TYR	HIS	PRO	ASP	VAL	ASN	LYS	SER
5	PRO	GLU	ALA	GLU	GLU	LYS	PHE	LYS	GLU	ILE
6	ASN	GLU	ALA	TYR	ALA	VAL	LEU	SER	ASP	PRO
7	GLU	LYS	ARG	ARG	ILE	TYR	ASP	THR	TYR	GLY
8	THR	THR	GLU	ALA	PRO	PRO	PRO	PRO	PRO	PRO
9	GLY	GLY	TYR	ASP	PHE	SER	GLY	PHE	ASP	VAL
10	GLU	ASP	PHE	SER	GLU	PHE	PHE	GLN	GLU	LEU
11	PHE	GLY	PRO	GLY	LEU	PHE	GLY	GLY	PHE	GLY
12	ARG	ARG	SER	ARG	LYS	GLY	ARG	ASP	LEU	ARG
13	ALA	GLU	LEU	PRO	LEU	THR	LEU	GLU	GLU	ALA
14	PHE	HIS	GLY	GLY	GLU	ARG	VAL	VAL	GLU	VAL
15	ALA	GLY	ARG	ARG	VAL	SER	VAL	ARG	ILE	PRO
16	PRO	GLY	VAL	ARG	GLU	GLY	SER	VAL	ILE	ARG
17	VAL	PRO	GLY	MET	GLY	GLY	GLN	GLY	ASN	PRO
18	PRO	GLY	ASP	LEU	LEU	LEU	VAL	VAL	ARG	LEU
19	LEU	PRO	HIS	PRO	VAL	PHE	ARG	LEU	GLU	GLY
20	GLN	ASP	LEU	TYR	ALA	THR	LEU	ASP	VAL	PRO
21	ALA	PRO	ILE	ALA	VAL	VAL	GLY	GLY	LYS	VAL
22	ARG	ALA	MET	THR	LEU	GLU	GLY	PRO	VAL	GLU
23	VAL	ALA	VAL	PRO	PRO	ARG	THR	GLN	ALA	GLY
24	ARG	LYS	LEU	ARG	LEU	LYS	GLY	LYS	GLY	PHE
25	PRO	GLY	PRO	ALA	GLY	ARG	GLY	ASP	LEU	TYR
26	LEU	GLU	VAL	ARG	ILE	THR	ILE	PRO	GLU	ARG
27	LEU	THR	PRO	GLU	GLU	GLU	ALA	LEU	TRP	LYS
28	LYS	LEU	ALA	GLU	ALA	TYR	TYR	ALA	ARG	ALA

1	MET	ALA	ALA	LYS	LYS	ASP	TYR	TYR	ALA	ILE
2	LEU	GLY	VAL	PRO	ARG	ASN	ALA	THR	GLN	GLU
3	GLU	ILE	LYS	ARG	ALA	TYR	LYS	ARG	LEU	ALA
4	ARG	GLN	TYR	HIS	PRO	ASP	VAL	ASN	LYS	SER
5	PRO	GLU	ALA	GLU	GLU	LYS	PHE	LYS	GLU	ILE
6	ASN	GLU	ALA	TYR	ALA	VAL	LEU	SER	ASP	PRO
7	GLU	LYS	ARG	ARG	ILE	TYR	ASP	THR	TYR	GLY
8	THR	THR	GLU	ALA	PRO	PRO	PRO	PRO	PRO	PRO
9	GLY	GLY	TYR	ASP	PHE	SER	GLY	PHE	ASP	VAL
10	GLU	ASP	PHE	SER	GLU	PHE	PHE	GLN	GLU	LEU
11	PHE	GLY	PRO	GLY	LEU	PHE	GLY	GLY	PHE	GLY
12	ARG	ARG	SER	ARG	LYS	GLY	ARG	ASP	LEU	ARG
13	ALA	GLU	LEU	PRO	LEU	THR	LEU	GLU	GLU	ALA
14	PHE	HIS	GLY	GLY	GLU	ARG	VAL	VAL	GLU	VAL
15	ALA	GLY	ARG	ARG	VAL	SER	VAL	ARG	ILE	PRO
16	PRO	GLY	VAL	ARG	GLU	GLY	SER	VAL	ILE	ARG
17	VAL	PRO	GLY	MET	GLY	GLY	GLN	GLY	ASN	PRO
18	PRO	GLY	ASP	LEU	LEU	LEU	VAL	VAL	ARG	LEU
19	LEU	PRO	HIS	PRO	VAL	PHE	ARG	LEU	GLU	GLY
20	GLN	ASP	LEU	TYR	ALA	THR	LEU	ASP	VAL	PRO
21	ALA	PRO	ILE	ALA	VAL	VAL	GLY	GLY	LYS	VAL
22	ARG	ALA	MET	THR	LEU	GLU	GLY	PRO	VAL	GLU
23	VAL	ALA	VAL	PRO	PRO	ARG	THR	GLN	ALA	GLY
24	ARG	LYS	LEU	ARG	LEU	LYS	GLY	LYS	GLY	PHE
25	PRO	GLY	PRO	ALA	GLY	ARG	GLY	ASP	LEU	TYR
26	LEU	GLU	VAL	ARG	ILE	THR	ILE	PRO	GLU	ARG
27	LEU	THR	PRO	GLU	GLU	GLU	ALA	LEU	TRP	LYS
28	LYS	LEU	ALA	GLU	ALA	TYR	TYR	ALA	ARG	ALA