BMRB Entry 52954

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52954

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Title:
Assignment of the Delta Prime Subunit in the Assembled Clamp Loader
Deposition date:
2025-03-07
Original release date:
2025-11-08
Authors:
Mahdi, Sam; Bezsonova, Irina; Beuning, Penny; Korzhnev, Dmitry
Citation:

Citation: Mahdi, Sam; Semenova, Irina; Bezsonova, Irina; Beuning, Penny; Korzhnev, Dmitry. "Divide-and-Conquer Strategy for NMR Studies of the E. coli g-Clamp Loader Complex"  J. Biomol. NMR ., .-. (2025).
PubMed: 40694188

Assembly members:

Assembly members:
entity_1, polymer, 334 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MRWYPWLRPDFEKLVASYQA GRGHHALLIQALPGMGDDAL IYALSRYLLCQQPQGHKSCG HCRGCQLMQAGTHPDYYTLA PEKGKNTLGVDAVREVTEKL NEHARLGGAKVVWVTDAALL TDAAANALLKTLEEPPAETW FFLATREPERLLATLRSRCR LHYLAPPPEQYAVTWLSREV TMSQDALLAALRLSAGSPGA ALALFQGDNWQARETLCQAL AYSVPSGDWYSLLAALNHEQ APARLHWLATLLMDALKRHH GAAQVTNVDVPGLVAELANH LSPSRLQAILGDVCHIREQL MSVTGINRELLITDLLLRIE HYLQPGVVLPVPHL

Data sets:
Data typeCount
13C chemical shifts93
1H chemical shifts279

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1the Delta Prime Subunit in the Assembled Clamp Loader1

Entities:

Entity 1, the Delta Prime Subunit in the Assembled Clamp Loader 334 residues - Formula weight is not available

1   METARGTRPTYRPROTRPLEUARGPROASP
2   PHEGLULYSLEUVALALASERTYRGLNALA
3   GLYARGGLYHISHISALALEULEUILEGLN
4   ALALEUPROGLYMETGLYASPASPALALEU
5   ILETYRALALEUSERARGTYRLEULEUCYS
6   GLNGLNPROGLNGLYHISLYSSERCYSGLY
7   HISCYSARGGLYCYSGLNLEUMETGLNALA
8   GLYTHRHISPROASPTYRTYRTHRLEUALA
9   PROGLULYSGLYLYSASNTHRLEUGLYVAL
10   ASPALAVALARGGLUVALTHRGLULYSLEU
11   ASNGLUHISALAARGLEUGLYGLYALALYS
12   VALVALTRPVALTHRASPALAALALEULEU
13   THRASPALAALAALAASNALALEULEULYS
14   THRLEUGLUGLUPROPROALAGLUTHRTRP
15   PHEPHELEUALATHRARGGLUPROGLUARG
16   LEULEUALATHRLEUARGSERARGCYSARG
17   LEUHISTYRLEUALAPROPROPROGLUGLN
18   TYRALAVALTHRTRPLEUSERARGGLUVAL
19   THRMETSERGLNASPALALEULEUALAALA
20   LEUARGLEUSERALAGLYSERPROGLYALA
21   ALALEUALALEUPHEGLNGLYASPASNTRP
22   GLNALAARGGLUTHRLEUCYSGLNALALEU
23   ALATYRSERVALPROSERGLYASPTRPTYR
24   SERLEULEUALAALALEUASNHISGLUGLN
25   ALAPROALAARGLEUHISTRPLEUALATHR
26   LEULEUMETASPALALEULYSARGHISHIS
27   GLYALAALAGLNVALTHRASNVALASPVAL
28   PROGLYLEUVALALAGLULEUALAASNHIS
29   LEUSERPROSERARGLEUGLNALAILELEU
30   GLYASPVALCYSHISILEARGGLUGLNLEU
31   METSERVALTHRGLYILEASNARGGLULEU
32   LEUILETHRASPLEULEULEUARGILEGLU
33   HISTYRLEUGLNPROGLYVALVALLEUPRO
34   VALPROHISLEU

Samples:

sample_1: The Delta Prime Subunit in the Assembled Clamp Loader, [U-13C; U-15N; U-2H; 99% 1HD-Ile,Leu; 99% 1HG-Val], 0.2 mM; Na2HPO4 50 mM; NaCl 100 mM; DTT 10 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
4D13CH3 HMQC-NOESY-HMQCsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz