BMRB Entry 52951

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52951

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Title:
Assignment of the Delta Subunit in the Assembled Clamp Loader
Deposition date:
2025-03-06
Original release date:
2025-11-08
Authors:
Mahdi, Sam; Bezsonova, Irina; Beuning, Penny; Korzhnev, Dmitry
Citation:

Citation: Mahdi, Sam; Semenova, Irina; Bezsonova, Irina; Beuning, Penny; Korzhnev, Dmitry. "Divide-and-Conquer Strategy for NMR Studies of the E. coli g-Clamp Loader Complex"  J. Biomol. NMR ., .-. (2025).
PubMed: 40694188

Assembly members:

Assembly members:
entity_1, polymer, 343 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETDuet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MIRLYPEQLRAQLNEGLRAA YLLLGNDPLLLQESQDAVRQ VAAAQGFEEHHTFSIDPNTD WNAIFSLCQAMSLFASRQTL LLLLPENGPNAAINEQLLTL TGLLHDDLLLIVRGNKLSKA QENAAWFTALANRSVQVTCQ TPEQAQLPRWVAARAKQLNL ELDDAANQVLCYCYEGNLLA LAQALERLSLLWPDGKLTLP RVEQAVNDAAHFTPFHWVDA LLMGKSKRALHILQQLRLEG SEPVILLRTLQRELLLLVNL KRQSAHTPLRALFDKHRVWQ NRRGMMGEALNRLSQTQLRQ AVQLLTRTELTLKQDYGLSV WAELEGLSLLLCHKPLADVF IDG

Data sets:
Data typeCount
13C chemical shifts114
1H chemical shifts342

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1The Delta Subunit in the Assembled Clamp Loader1

Entities:

Entity 1, The Delta Subunit in the Assembled Clamp Loader 343 residues - Formula weight is not available

1   METILEARGLEUTYRPROGLUGLNLEUARG
2   ALAGLNLEUASNGLUGLYLEUARGALAALA
3   TYRLEULEULEUGLYASNASPPROLEULEU
4   LEUGLNGLUSERGLNASPALAVALARGGLN
5   VALALAALAALAGLNGLYPHEGLUGLUHIS
6   HISTHRPHESERILEASPPROASNTHRASP
7   TRPASNALAILEPHESERLEUCYSGLNALA
8   METSERLEUPHEALASERARGGLNTHRLEU
9   LEULEULEULEUPROGLUASNGLYPROASN
10   ALAALAILEASNGLUGLNLEULEUTHRLEU
11   THRGLYLEULEUHISASPASPLEULEULEU
12   ILEVALARGGLYASNLYSLEUSERLYSALA
13   GLNGLUASNALAALATRPPHETHRALALEU
14   ALAASNARGSERVALGLNVALTHRCYSGLN
15   THRPROGLUGLNALAGLNLEUPROARGTRP
16   VALALAALAARGALALYSGLNLEUASNLEU
17   GLULEUASPASPALAALAASNGLNVALLEU
18   CYSTYRCYSTYRGLUGLYASNLEULEUALA
19   LEUALAGLNALALEUGLUARGLEUSERLEU
20   LEUTRPPROASPGLYLYSLEUTHRLEUPRO
21   ARGVALGLUGLNALAVALASNASPALAALA
22   HISPHETHRPROPHEHISTRPVALASPALA
23   LEULEUMETGLYLYSSERLYSARGALALEU
24   HISILELEUGLNGLNLEUARGLEUGLUGLY
25   SERGLUPROVALILELEULEUARGTHRLEU
26   GLNARGGLULEULEULEULEUVALASNLEU
27   LYSARGGLNSERALAHISTHRPROLEUARG
28   ALALEUPHEASPLYSHISARGVALTRPGLN
29   ASNARGARGGLYMETMETGLYGLUALALEU
30   ASNARGLEUSERGLNTHRGLNLEUARGGLN
31   ALAVALGLNLEULEUTHRARGTHRGLULEU
32   THRLEULYSGLNASPTYRGLYLEUSERVAL
33   TRPALAGLULEUGLUGLYLEUSERLEULEU
34   LEUCYSHISLYSPROLEUALAASPVALPHE
35   ILEASPGLY

Samples:

sample_1: The Delta Subunit in the Assembeled Clamp Loader, [U-13C; U-15N; U-2H; 99% 1HD-Ile,Leu; 99% 1HG-Val], 0.2 mM; Na2HPO4 50 mM; NaCl 100 mM; DTT 10 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
4D 13CH3 HMQC-NOESY-HMQCsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY v1.4 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz