BMRB Entry 52947

Name: Backbone assignment Sex-lethal dRBD5 (122-305)
Published: 2025-07-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52947

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment Sex-lethal dRBD5 (122-305)

Deposition date:

2025-03-06

Original release date:

2025-07-04

Authors:

Meyer, Julia; Hennig, Janosch

Citation:

Citation: Payr, Marco; Meyer, Julia; Geissen, Eva-Maria; Duss, Olivier; Hennig, Janosch. "Real-time tracking of mRNP complex assembly reveals multiple mechanisms ensuring efficient translation repression" .

Assembly members:

Assembly members:
entity_1, polymer, 186 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24d(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAASNTNLIVNYLPQDMTDR ELYALFRAIGPINTCRIMRD YKTGYSFGYAFVDFTSEMDS QRAIKVLNGITVRNKRLKVS YARPGGESIKDTNLYVTNLP RTITDDQLDTIFGKYGSIVQ KNILRDKLTGRPRGVAFVRY NKREEAQEAISALNNVIPEG GSQPLSVRLAEEHGKAKAAH FMSQMG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	508
15N chemical shifts	175
1H chemical shifts	175

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sex-lethal dRBD5	1

Entities:

Entity 1, Sex-lethal dRBD5 186 residues - Formula weight is not available

N-terminal GA from purification tag

1

GLY

ALA

SER

ASN

THR

ASN

LEU

ILE

VAL

2

ASN

TYR

LEU

PRO

GLN

ASP

MET

THR

ASP

ARG

3

GLU

LEU

TYR

ALA

LEU

PHE

ARG

ALA

ILE

GLY

4

PRO

ILE

ASN

THR

CYS

ARG

ILE

MET

ARG

ASP

5

TYR

LYS

THR

GLY

TYR

SER

PHE

GLY

TYR

ALA

6

PHE

VAL

ASP

PHE

THR

SER

GLU

MET

ASP

SER

7

GLN

ARG

ALA

ILE

LYS

VAL

LEU

ASN

GLY

ILE

8

THR

VAL

ARG

ASN

LYS

ARG

LEU

LYS

VAL

SER

9

TYR

ALA

ARG

PRO

GLY

GLU

SER

ILE

LYS

10

ASP

THR

ASN

LEU

TYR

VAL

THR

ASN

LEU

PRO

11

ARG

THR

ILE

THR

ASP

GLN

LEU

ASP

THR

12

ILE

PHE

GLY

LYS

TYR

GLY

SER

ILE

VAL

GLN

13

LYS

ASN

ILE

LEU

ARG

ASP

LYS

LEU

THR

GLY

14

ARG

PRO

ARG

GLY

VAL

ALA

PHE

VAL

ARG

TYR

15

ASN

LYS

ARG

GLU

ALA

GLN

GLU

ALA

ILE

16

SER

ALA

LEU

ASN

VAL

ILE

PRO

GLU

GLY

17

GLY

SER

GLN

PRO

LEU

SER

VAL

ARG

LEU

ALA

18

GLU

HIS

GLY

LYS

ALA

LYS

ALA

HIS

19

PHE

MET

SER

GLN

MET

GLY

Samples:

sample_1: Sex-lethal dRBD5, [U-13C; U-15N; U-2H], 300 uM; potassium phosphate 10 mM; sodium chloride 50 mM; DTT 1 mM

sample_conditions_1: pH: 6.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

NMRViewJ - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 1000 MHz
Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks