BMRB Entry 5294

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5294
MolProbity Validation Chart

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Title:
Structure of the beta subunit of translation initiation factor 2 from the archaeon Methanococcus jannaschii: A representative of the eIF2beta/eIF5 family of proteins
Deposition date:
2002-02-18
Original release date:
2002-09-11
Authors:
Cho, Seongeun; Hoffman, David
Citation:

Citation: Cho, Seongeun; Hoffman, David. "Structure of the Beta Subunit of Translation Initiation Factor 2 from the Archaeon Methanococcus jannaschii: A Representative of the eIF2beta/eIF5 Family of Proteins "  Biochemistry 41, 5730-5742 (2002).
PubMed: 11980477

Assembly members:

Assembly members:
translation initiation factor IF2b, polymer, 143 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Methanococcus jannaschii   Taxonomy ID: 2190   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanococcus jannaschii

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
translation initiation factor IF2b: MSDLENIDYYDYKALLKRAR SQIPDYVFQKDRFELPEIEI LIEGNRTIIRNFRELAKAVN RDEEFFAKYLLKETGSAGNL EGGRLILQRRISPELLKSRI NDFLREYVICRECGKPDTKI IKEGRVHLLKCMACGAIRPI RMI

Data sets:
Data typeCount
1H chemical shifts485
13C chemical shifts181
15N chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1aIF2b1
2ZINC (II) ION2

Entities:

Entity 1, aIF2b 143 residues - Formula weight is not available

1   METSERASPLEUGLUASNILEASPTYRTYR
2   ASPTYRLYSALALEULEULYSARGALAARG
3   SERGLNILEPROASPTYRVALPHEGLNLYS
4   ASPARGPHEGLULEUPROGLUILEGLUILE
5   LEUILEGLUGLYASNARGTHRILEILEARG
6   ASNPHEARGGLULEUALALYSALAVALASN
7   ARGASPGLUGLUPHEPHEALALYSTYRLEU
8   LEULYSGLUTHRGLYSERALAGLYASNLEU
9   GLUGLYGLYARGLEUILELEUGLNARGARG
10   ILESERPROGLULEULEULYSSERARGILE
11   ASNASPPHELEUARGGLUTYRVALILECYS
12   ARGGLUCYSGLYLYSPROASPTHRLYSILE
13   ILELYSGLUGLYARGVALHISLEULEULYS
14   CYSMETALACYSGLYALAILEARGPROILE
15   ARGMETILE

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: translation initiation factor IF2b, [U-13C; U-15N], 0.5 – 1.5 mM; ZINC (II) ION mM

sample_2: translation initiation factor IF2b, [U-15N], 0.5 – 1.5 mM; ZINC (II) ION mM

condition_1: pH: 6.0 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablecondition_1
HNCOnot availablenot availablecondition_1
HNCACBnot availablenot availablecondition_1
HN(CO)CACBnot availablenot availablecondition_1
3D-HCCH-TOCSYnot availablenot availablecondition_1
15N-HMQCnot availablenot availablecondition_1
3D 1H-1H-15N NOESYnot availablenot availablecondition_1
3D 1H-13C-1H HCCH-TOCSYnot availablenot availablecondition_1

Software:

felix - processing

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

GB AAB98077 ACV24219 ADC69390 AIJ05339
REF NP_247061 WP_010869589 WP_012980300 WP_015790959 YP_003127719
SP Q57562
AlphaFold Q57562 Q57562

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks