BMRB Entry 52921

Name: NMR assignments of the Fc fragment of human immunoglobulin G1 glycoprotein non-fucosylated, non-galactosylated (G0) glycoform-2
Published: 2025-07-22

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52921

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

NMR assignments of the Fc fragment of human immunoglobulin G1 glycoprotein non-fucosylated, non-galactosylated (G0) glycoform-2

Deposition date:

2025-02-21

Original release date:

2025-07-22

Authors:

Yanaka, Saeko; Kato, Koichi

Citation:

Citation: Yanaka, Saeko; Sakae, Yoshitake; Miyanoiri, Yohei; Yamaguchi, Takumi; Isono, Yukiko; Kondo, Sachiko; Iwasaki, Miyuki; Onitsuka, Masayoshi; Yagi, Hirokazu; Kato, Koichi. "Exploring Glycoform-Dependent Dynamic Modulations in the Fc Region of Human Immunoglobulin G via Computational and Experimental Approaches" .

Assembly members:

Assembly members:
entity_1, polymer, 230 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Cricetulus griseus Vector: none

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TCPPCPAPELLGGPSVFLFP PKPKDTLMISRTPEVTCVVV DVSHEDPEVKFNWYVDGVEV HNAKTKPREEQYNSTYRVVS VLTVLHQDWLNGKEYKCKVS NKALPAPIEKTISKAKGQPR EPQVYTLPPSRDELTKNQVS LTCLVKGFYPSDIAVEWESN GQNENNYKTTPPVLDSDGSF FLYSKLTVDKSRWQQGNVFS CSVMHEALHNHYTQKSLSLS PGKXXXXXXX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	32
15N chemical shifts	186
1H chemical shifts	208

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 230 residues - Formula weight is not available

1	THR	CYS	PRO	PRO	CYS	PRO	ALA	PRO	GLU	LEU
2	LEU	GLY	GLY	PRO	SER	VAL	PHE	LEU	PHE	PRO
3	PRO	LYS	PRO	LYS	ASP	THR	LEU	MET	ILE	SER
4	ARG	THR	PRO	GLU	VAL	THR	CYS	VAL	VAL	VAL
5	ASP	VAL	SER	HIS	GLU	ASP	PRO	GLU	VAL	LYS
6	PHE	ASN	TRP	TYR	VAL	ASP	GLY	VAL	GLU	VAL
7	HIS	ASN	ALA	LYS	THR	LYS	PRO	ARG	GLU	GLU
8	GLN	TYR	ASN	SER	THR	TYR	ARG	VAL	VAL	SER
9	VAL	LEU	THR	VAL	LEU	HIS	GLN	ASP	TRP	LEU
10	ASN	GLY	LYS	GLU	TYR	LYS	CYS	LYS	VAL	SER
11	ASN	LYS	ALA	LEU	PRO	ALA	PRO	ILE	GLU	LYS
12	THR	ILE	SER	LYS	ALA	LYS	GLY	GLN	PRO	ARG
13	GLU	PRO	GLN	VAL	TYR	THR	LEU	PRO	PRO	SER
14	ARG	ASP	GLU	LEU	THR	LYS	ASN	GLN	VAL	SER
15	LEU	THR	CYS	LEU	VAL	LYS	GLY	PHE	TYR	PRO
16	SER	ASP	ILE	ALA	VAL	GLU	TRP	GLU	SER	ASN
17	GLY	GLN	ASN	GLU	ASN	ASN	TYR	LYS	THR	THR
18	PRO	PRO	VAL	LEU	ASP	SER	ASP	GLY	SER	PHE
19	PHE	LEU	TYR	SER	LYS	LEU	THR	VAL	ASP	LYS
20	SER	ARG	TRP	GLN	GLN	GLY	ASN	VAL	PHE	SER
21	CYS	SER	VAL	MET	HIS	GLU	ALA	LEU	HIS	ASN
22	HIS	TYR	THR	GLN	LYS	SER	LEU	SER	LEU	SER
23	PRO	GLY	LYS	GN1	GN2	M3	M4	GN5	M4	GN5

Samples:

sample_1: Fc fragment of human immunoglobulin G1 glycoprotein non-fucosylated, non-galactosylated (G0) glycoform, [U-100% 15N], 0.4 mM; NaCl 50 mM; sodium phosphate 5 mM

sample_conditions_1: pH: 6.7; pressure: 1 atm; temperature: 315 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
1H-13C HMBC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC-TOCSY	sample_1	isotropic	sample_conditions_1
2D COSY	sample_1	isotropic	sample_conditions_1
2D TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, SPARKY - chemical shift assignment, collection, data analysis, processing

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	THR	CYS	PRO	PRO	CYS	PRO	ALA	PRO	GLU	LEU
2	LEU	GLY	GLY	PRO	SER	VAL	PHE	LEU	PHE	PRO
3	PRO	LYS	PRO	LYS	ASP	THR	LEU	MET	ILE	SER
4	ARG	THR	PRO	GLU	VAL	THR	CYS	VAL	VAL	VAL
5	ASP	VAL	SER	HIS	GLU	ASP	PRO	GLU	VAL	LYS
6	PHE	ASN	TRP	TYR	VAL	ASP	GLY	VAL	GLU	VAL
7	HIS	ASN	ALA	LYS	THR	LYS	PRO	ARG	GLU	GLU
8	GLN	TYR	ASN	SER	THR	TYR	ARG	VAL	VAL	SER
9	VAL	LEU	THR	VAL	LEU	HIS	GLN	ASP	TRP	LEU
10	ASN	GLY	LYS	GLU	TYR	LYS	CYS	LYS	VAL	SER
11	ASN	LYS	ALA	LEU	PRO	ALA	PRO	ILE	GLU	LYS
12	THR	ILE	SER	LYS	ALA	LYS	GLY	GLN	PRO	ARG
13	GLU	PRO	GLN	VAL	TYR	THR	LEU	PRO	PRO	SER
14	ARG	ASP	GLU	LEU	THR	LYS	ASN	GLN	VAL	SER
15	LEU	THR	CYS	LEU	VAL	LYS	GLY	PHE	TYR	PRO
16	SER	ASP	ILE	ALA	VAL	GLU	TRP	GLU	SER	ASN
17	GLY	GLN	ASN	GLU	ASN	ASN	TYR	LYS	THR	THR
18	PRO	PRO	VAL	LEU	ASP	SER	ASP	GLY	SER	PHE
19	PHE	LEU	TYR	SER	LYS	LEU	THR	VAL	ASP	LYS
20	SER	ARG	TRP	GLN	GLN	GLY	ASN	VAL	PHE	SER
21	CYS	SER	VAL	MET	HIS	GLU	ALA	LEU	HIS	ASN
22	HIS	TYR	THR	GLN	LYS	SER	LEU	SER	LEU	SER
23	PRO	GLY	LYS	GN1	GN2	M3	M4	GN5	M4	GN5

1	THR	CYS	PRO	PRO	CYS	PRO	ALA	PRO	GLU	LEU
2	LEU	GLY	GLY	PRO	SER	VAL	PHE	LEU	PHE	PRO
3	PRO	LYS	PRO	LYS	ASP	THR	LEU	MET	ILE	SER
4	ARG	THR	PRO	GLU	VAL	THR	CYS	VAL	VAL	VAL
5	ASP	VAL	SER	HIS	GLU	ASP	PRO	GLU	VAL	LYS
6	PHE	ASN	TRP	TYR	VAL	ASP	GLY	VAL	GLU	VAL
7	HIS	ASN	ALA	LYS	THR	LYS	PRO	ARG	GLU	GLU
8	GLN	TYR	ASN	SER	THR	TYR	ARG	VAL	VAL	SER
9	VAL	LEU	THR	VAL	LEU	HIS	GLN	ASP	TRP	LEU
10	ASN	GLY	LYS	GLU	TYR	LYS	CYS	LYS	VAL	SER
11	ASN	LYS	ALA	LEU	PRO	ALA	PRO	ILE	GLU	LYS
12	THR	ILE	SER	LYS	ALA	LYS	GLY	GLN	PRO	ARG
13	GLU	PRO	GLN	VAL	TYR	THR	LEU	PRO	PRO	SER
14	ARG	ASP	GLU	LEU	THR	LYS	ASN	GLN	VAL	SER
15	LEU	THR	CYS	LEU	VAL	LYS	GLY	PHE	TYR	PRO
16	SER	ASP	ILE	ALA	VAL	GLU	TRP	GLU	SER	ASN
17	GLY	GLN	ASN	GLU	ASN	ASN	TYR	LYS	THR	THR
18	PRO	PRO	VAL	LEU	ASP	SER	ASP	GLY	SER	PHE
19	PHE	LEU	TYR	SER	LYS	LEU	THR	VAL	ASP	LYS
20	SER	ARG	TRP	GLN	GLN	GLY	ASN	VAL	PHE	SER
21	CYS	SER	VAL	MET	HIS	GLU	ALA	LEU	HIS	ASN
22	HIS	TYR	THR	GLN	LYS	SER	LEU	SER	LEU	SER
23	PRO	GLY	LYS	GN1	GN2	M3	M4	GN5	M4	GN5

1	THR	CYS	PRO	PRO	CYS	PRO	ALA	PRO	GLU	LEU
2	LEU	GLY	GLY	PRO	SER	VAL	PHE	LEU	PHE	PRO
3	PRO	LYS	PRO	LYS	ASP	THR	LEU	MET	ILE	SER
4	ARG	THR	PRO	GLU	VAL	THR	CYS	VAL	VAL	VAL
5	ASP	VAL	SER	HIS	GLU	ASP	PRO	GLU	VAL	LYS
6	PHE	ASN	TRP	TYR	VAL	ASP	GLY	VAL	GLU	VAL
7	HIS	ASN	ALA	LYS	THR	LYS	PRO	ARG	GLU	GLU
8	GLN	TYR	ASN	SER	THR	TYR	ARG	VAL	VAL	SER
9	VAL	LEU	THR	VAL	LEU	HIS	GLN	ASP	TRP	LEU
10	ASN	GLY	LYS	GLU	TYR	LYS	CYS	LYS	VAL	SER
11	ASN	LYS	ALA	LEU	PRO	ALA	PRO	ILE	GLU	LYS
12	THR	ILE	SER	LYS	ALA	LYS	GLY	GLN	PRO	ARG
13	GLU	PRO	GLN	VAL	TYR	THR	LEU	PRO	PRO	SER
14	ARG	ASP	GLU	LEU	THR	LYS	ASN	GLN	VAL	SER
15	LEU	THR	CYS	LEU	VAL	LYS	GLY	PHE	TYR	PRO
16	SER	ASP	ILE	ALA	VAL	GLU	TRP	GLU	SER	ASN
17	GLY	GLN	ASN	GLU	ASN	ASN	TYR	LYS	THR	THR
18	PRO	PRO	VAL	LEU	ASP	SER	ASP	GLY	SER	PHE
19	PHE	LEU	TYR	SER	LYS	LEU	THR	VAL	ASP	LYS
20	SER	ARG	TRP	GLN	GLN	GLY	ASN	VAL	PHE	SER
21	CYS	SER	VAL	MET	HIS	GLU	ALA	LEU	HIS	ASN
22	HIS	TYR	THR	GLN	LYS	SER	LEU	SER	LEU	SER
23	PRO	GLY	LYS	GN1	GN2	M3	M4	GN5	M4	GN5