BMRB Entry 52917

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52917

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Title:
NMR assignments of the Fc fragment of human immunoglobulin G1 glycoprotein fucosylated, non-galactosylated (FG0) glycoform
Deposition date:
2025-02-20
Original release date:
2025-07-22
Authors:
Yanaka, Saeko; Kato, Koichi
Citation:

Citation: Yanaka, Saeko; Sakae, Yoshitake; Miyanoiri, Yohei; Yamaguchi, Takumi; Isono, Yukiko; Kondo, Sachiko; Iwasaki, Miyuki; Onitsuka, Masayoshi; Yagi, Hirokazu; Kato, Koichi. "Exploring Glycoform-Dependent Dynamic Modulations in the Fc Region of Human Immunoglobulin G via Computational and Experimental Approaches"  .

Assembly members:

Assembly members:
entity_1, polymer, 231 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Cricetulus griseus   Vector: none

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TCPPCPAPELLGGPSVFLFK PKPKDTLMISRTPEVTCVVV DVSHEDPEVKFNWYVDGVEV HNAKTKPREEQYNSTYRVVS VLTVLHQDWLNGKEYKCKVS NKALPAPIEKTISKAKGQPR EPQVYTLPPSRDELTKNQVS LTCLVKGFYPSDIAVEWESN GQPENNYKTTPPVLDSDGSF FLYSKLTVDKSRWQQGNVFS CSVMHEALHNHYTQKSLSLS PGKXXXXXXXX

Data sets:
Data typeCount
13C chemical shifts37
15N chemical shifts174
1H chemical shifts201

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FG01

Entities:

Entity 1, FG0 231 residues - Formula weight is not available

1   THRCYSPROPROCYSPROALAPROGLULEU
2   LEUGLYGLYPROSERVALPHELEUPHELYS
3   PROLYSPROLYSASPTHRLEUMETILESER
4   ARGTHRPROGLUVALTHRCYSVALVALVAL
5   ASPVALSERHISGLUASPPROGLUVALLYS
6   PHEASNTRPTYRVALASPGLYVALGLUVAL
7   HISASNALALYSTHRLYSPROARGGLUGLU
8   GLNTYRASNSERTHRTYRARGVALVALSER
9   VALLEUTHRVALLEUHISGLNASPTRPLEU
10   ASNGLYLYSGLUTYRLYSCYSLYSVALSER
11   ASNLYSALALEUPROALAPROILEGLULYS
12   THRILESERLYSALALYSGLYGLNPROARG
13   GLUPROGLNVALTYRTHRLEUPROPROSER
14   ARGASPGLULEUTHRLYSASNGLNVALSER
15   LEUTHRCYSLEUVALLYSGLYPHETYRPRO
16   SERASPILEALAVALGLUTRPGLUSERASN
17   GLYGLNPROGLUASNASNTYRLYSTHRTHR
18   PROPROVALLEUASPSERASPGLYSERPHE
19   PHELEUTYRSERLYSLEUTHRVALASPLYS
20   SERARGTRPGLNGLNGLYASNVALPHESER
21   CYSSERVALMETHISGLUALALEUHISASN
22   HISTYRTHRGLNLYSSERLEUSERLEUSER
23   PROGLYLYSGN1GN2M3M4GN5M4GN5
24   FUC

Samples:

sample_1: Fc fragment of human immunoglobulin G1 glycoprotein fucosylated, non-galactosylated (FG0) glycoform, [U-100% 13C; U-100% 15N], 0.4 mM; sodium phosphate 5 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6.7; temperature: 315 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
1H-13C HMBCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC-TOCSYsample_1isotropicsample_conditions_1
2D COSYsample_1isotropicsample_conditions_1
2D TOCSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, SPARKY - chemical shift assignment, collection, data analysis, processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks