BMRB Entry 5288

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5288

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Title:
Assignment of Backbone 1H, 13C, and 15N Resonances of Human Grb7-SH2 Domain in Complex with a Phosphorylated Peptide Ligand
Deposition date:
2002-02-14
Original release date:
2002-08-23
Authors:
Brescoa, Peter; Ivancic, Monika; Lyons, Barbara
Citation:

Citation: Brescoa, Peter; Ivancic, Monika; Lyons, Barbara. "Letter to the Editor: Assignment of Backbone 1H, 13C, and 15N Resonances of Human Grb7-SH2 Domain in Complex with a Phosphorylated Peptide Ligand "  J. Biomol. NMR 23, 77-78 (2002).
PubMed: 12061724

Assembly members:

Assembly members:
growth factor receptor bound protein 7, polymer, 120 residues, 13672 Da.
phosphorylated peptide, pY1139, polymer, . residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapien

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
growth factor receptor bound protein 7: GSPASGTSLSAAIHRTQLWF HGRISREESQRLIGQQGLVD GLFLVRESQRNPQGFVLSLC HLQKVKHYLILPSEEEGRLY FSMDDGQTRFTDLLQLVEFH QLNRGILPCLLRHCCTRVAL
phosphorylated peptide, pY1139: X

Data sets:
Data typeCount
1H chemical shifts114
13C chemical shifts198
15N chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Grb7 SH2 domain1
2phosphorylated peptide, pY11392

Entities:

Entity 1, Grb7 SH2 domain 120 residues - 13672 Da.

1   GLYSERPROALASERGLYTHRSERLEUSER
2   ALAALAILEHISARGTHRGLNLEUTRPPHE
3   HISGLYARGILESERARGGLUGLUSERGLN
4   ARGLEUILEGLYGLNGLNGLYLEUVALASP
5   GLYLEUPHELEUVALARGGLUSERGLNARG
6   ASNPROGLNGLYPHEVALLEUSERLEUCYS
7   HISLEUGLNLYSVALLYSHISTYRLEUILE
8   LEUPROSERGLUGLUGLUGLYARGLEUTYR
9   PHESERMETASPASPGLYGLNTHRARGPHE
10   THRASPLEULEUGLNLEUVALGLUPHEHIS
11   GLNLEUASNARGGLYILELEUPROCYSLEU
12   LEUARGHISCYSCYSTHRARGVALALALEU

Entity 2, phosphorylated peptide, pY1139 - Formula weight is not available

1   PTR

Samples:

sample_1: growth factor receptor bound protein 7, [U-98% 13C; U-98% 15N], 0.8 mM

sample_2: growth factor receptor bound protein 7, [U-98% 13C; U-98% 15N], 0.8 mM; phosphorylated peptide, pY1139 0.8 mM

EX-cond_1: pH: 6.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availableEX-cond_1
HNCAnot availablenot availableEX-cond_1
HNCOCAnot availablenot availableEX-cond_1
CBCACONHnot availablenot availableEX-cond_1
HNCACBnot availablenot availableEX-cond_1

Software:

AZARA - data processing, assignment analysis

ANSIG - data processing, assignment analysis

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

REF XP_001091846 XP_001091612 XP_001091728 NP_001025173 NP_005301
SWISS-PROT Q14451
GenBank AAP36681 AAX29563 AAH06535 AAP35332 AAG25938
DBJ BAF82804 BAD96569 BAD96590 BAA07827 BAA29059
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks