BMRB Entry 52877

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for p53 TAD
Published: 2025-02-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52877

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for p53 TAD

Deposition date:

2025-01-21

Original release date:

2025-02-24

Authors:

Sebak, Fanni; Bodor, Andrea

Citation:

Citation: Dudas, Erika; Palfy, Gyula; Menyhard, Dora; Sebak, Fanni; Ecsedi, Peter; Nyitray, Laszlo; Bodor, Andrea. "Tumor-Suppressor p53TAD" Chembiochem 21, 3087-3095 (2020).
PubMed: 32511842

Assembly members:

Assembly members:
entity_1, polymer, 62 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETARA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSMEEPQSDPSVEPPLSQET FSDLWKLLPENNVLSPLPSQ AMDDLMLSPDDIEQWFTEDP GP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	166
15N chemical shifts	51
1H chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p53 TAD	1

Entities:

Entity 1, p53 TAD 62 residues - Formula weight is not available

Residues -1-0 represent a non-native affinity tag.

1

GLY

SER

MET

GLU

PRO

GLN

SER

ASP

PRO

2

SER

VAL

GLU

PRO

LEU

SER

GLN

GLU

THR

3

PHE

SER

ASP

LEU

TRP

LYS

LEU

PRO

GLU

4

ASN

VAL

LEU

SER

PRO

LEU

PRO

SER

GLN

5

ALA

MET

ASP

LEU

MET

LEU

SER

PRO

ASP

6

ASP

ILE

GLU

GLN

TRP

PHE

THR

GLU

ASP

PRO

7

GLY

PRO

Samples:

sample_1: p53 TAD, [U-100% 13C; U-100% 15N], 1 mM; D2O, [U-100% 2H], 10%; MES 20 mM; sodium chloride 20 mM; TCEP 10 mM; sodium azide 3 mM; calcium chloride 10 mM; DSS 5 uL

sample_conditions_1: pH: 5.8; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.6 - processing

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks