BMRB Entry 52864

Name: Eps15 IDR 569-671
Published: 2025-08-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52864

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Eps15 IDR 569-671

Deposition date:

2025-01-15

Original release date:

2025-08-18

Authors:

Papagiannoula, Andromachi; Vedel, Ida; Motzny, Kathrin; Tengo, Maud; Saiti, Arbesa; Milles, Sigrid

Citation:

Citation: Papagiannoula, Andromachi; Vedel, Ida; Motzny, Kathrin; Tengo, Maud; Saiti, Arbesa; Milles, Sigrid. "Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network" Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMSGVTDENEVTTAVTEKV CSELDNNRHSKEEDPFNVDS SSLTGPVADTNLDFFQSDPF VGSDPFKDDPFGKIDPFGGD PFKGSDPFASDCFFRQSTDP FATSST

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	280
15N chemical shifts	87
1H chemical shifts	87

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Eps15 IDR 569-671	1

Entities:

Entity 1, Eps15 IDR 569-671 106 residues - Formula weight is not available

1

GLY

HIS

MET

SER

GLY

VAL

THR

ASP

GLU

ASN

2

GLU

VAL

THR

ALA

VAL

THR

GLU

LYS

VAL

3

CYS

SER

GLU

LEU

ASP

ASN

ARG

HIS

SER

4

LYS

GLU

ASP

PRO

PHE

ASN

VAL

ASP

SER

5

SER

LEU

THR

GLY

PRO

VAL

ALA

ASP

THR

6

ASN

LEU

ASP

PHE

GLN

SER

ASP

PRO

PHE

7

VAL

GLY

SER

ASP

PRO

PHE

LYS

ASP

PRO

8

PHE

GLY

LYS

ILE

ASP

PRO

PHE

GLY

ASP

9

PRO

PHE

LYS

GLY

SER

ASP

PRO

PHE

ALA

SER

10

ASP

CYS

PHE

ARG

GLN

SER

THR

ASP

PRO

11

PHE

ALA

THR

SER

THR

Samples:

sample_1: Eps15 IDR 569-671, [U-99% 13C; U-99% 15N], 600 uM; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 211.6 mM; pH: 6; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
HNCOCANNHGPWG3D	sample_1	isotropic	sample_conditions_1
HNCANNHGPWG3D	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

qMDD - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks