BMRB Entry 52863

Name: Chemical Shift Assignments of Eps15 IDR 648-780
Published: 2025-08-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52863

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignments of Eps15 IDR 648-780

Deposition date:

2025-01-15

Original release date:

2025-08-18

Authors:

Papagiannoula, Andromachi; Vedel, Ida; Motzny, Kathrin; Tengo, Maud; Saiti, Arbesa; Milles, Sigrid

Citation:

Citation: Papagiannoula, Andromachi; Vedel, Ida; Motzny, Kathrin; Tengo, Maud; Saiti, Arbesa; Milles, Sigrid. "Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network" Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMKGSDPFASDCFFRQSTD PFATSSTDPFSAANNSSITS VETLKHNDPFAPGGTVVAAS DSATDPFASVFGNESFGGGF ADFSTLSKVNNEDPFRSATS SSVSNVVITKNVFEETSVKS EDEPPALPPKIGTPTR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	214
15N chemical shifts	70
1H chemical shifts	70

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Eps15 IDR 648-780	1

Entities:

Entity 1, Eps15 IDR 648-780 136 residues - Formula weight is not available

1

GLY

HIS

MET

LYS

GLY

SER

ASP

PRO

PHE

ALA

2

SER

ASP

CYS

PHE

ARG

GLN

SER

THR

ASP

3

PRO

PHE

ALA

THR

SER

THR

ASP

PRO

PHE

4

SER

ALA

ASN

SER

ILE

THR

SER

5

VAL

GLU

THR

LEU

LYS

HIS

ASN

ASP

PRO

PHE

6

ALA

PRO

GLY

THR

VAL

ALA

SER

7

ASP

SER

ALA

THR

ASP

PRO

PHE

ALA

SER

VAL

8

PHE

GLY

ASN

GLU

SER

PHE

GLY

PHE

9

ALA

ASP

PHE

SER

THR

LEU

SER

LYS

VAL

ASN

10

ASN

GLU

ASP

PRO

PHE

ARG

SER

ALA

THR

SER

11

SER

VAL

SER

ASN

VAL

ILE

THR

LYS

12

ASN

VAL

PHE

GLU

THR

SER

VAL

LYS

SER

13

GLU

ASP

GLU

PRO

ALA

LEU

PRO

LYS

14

ILE

GLY

THR

PRO

THR

ARG

Samples:

sample_1: Eps15 IDR 648-780, [U-99% 13C; U-99% 15N], 550 uM; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 211.6 mM; pH: 6; pressure: 1 atm; temperature: 298.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

CcpNMR - chemical shift assignment

NMRPipe - processing

qMDD - processing

NMR spectrometers:

Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks