BMRB Entry 52829

Name: SN2 (141-204) from SNAP25
Published: 2025-01-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52829

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SN2 (141-204) from SNAP25

Deposition date:

2025-01-12

Original release date:

2025-01-28

Authors:

Kraichely, Katelyn; Sandall, Connor; Liang, Binyong; Kiessling, Volker; Tamm, Lukas

Citation:

Citation: Kraichely, Katelyn; Sandall, Connor; Liang, Binyong; Kiessling, Volker; Tamm, Lukas. "Functionally distinct SNARE motifs of SNAP25 cooperate in SNARE assembly and membrane fusion" Biophys. J. 124, 637-650 (2025).
PubMed: 39982442

Assembly members:

Assembly members:
entity_1, polymer, 64 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ARENEMDENLEQVSDIIGNL RHMALDMGNEIDTQNRQIDR IMEKADSNKTRIDEANQRAT KMLG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	189
15N chemical shifts	64
1H chemical shifts	64

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SN2	1

Entities:

Entity 1, SN2 64 residues - Formula weight is not available

1

ALA

ARG

GLU

ASN

GLU

MET

ASP

GLU

ASN

LEU

2

GLU

GLN

VAL

SER

ASP

ILE

GLY

ASN

LEU

3

ARG

HIS

MET

ALA

LEU

ASP

MET

GLY

ASN

GLU

4

ILE

ASP

THR

GLN

ASN

ARG

GLN

ILE

ASP

ARG

5

ILE

MET

GLU

LYS

ALA

ASP

SER

ASN

LYS

THR

6

ARG

ILE

ASP

GLU

ALA

ASN

GLN

ARG

ALA

THR

7

LYS

MET

LEU

GLY

Samples:

sample_1: SN2, [U-99% 13C; U-99% 15N], 1 mM; HEPES 10 mM; MES 10 mM; sodium acetate 10 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCB	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

SPARKY - data analysis

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks