BMRB Entry 52828

Name: Functionally Distinct SNARE Motifs of SNAP25 Cooperate in SNARE Assembly and Membrane Fusion: SN1
Published: 2025-01-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52828

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Functionally Distinct SNARE Motifs of SNAP25 Cooperate in SNARE Assembly and Membrane Fusion: SN1

Deposition date:

2025-01-12

Original release date:

2025-01-28

Authors:

Kraichely, Katelyn; Sandall, Connor; Liang, Binyong; Kiessling, Volker; Tamm, Lukas

Citation:

Citation: Kraichely, Katelyn; Sandall, Connor; Liang, Binyong; Kiessling, Volker; Tamm, Lukas. "Functionally distinct SNARE motifs of SNAP25 cooperate in SNARE assembly and membrane fusion" Biophys. J. 124, 637-650 (2025).
PubMed: 39982442

Assembly members:

Assembly members:
entity_1, polymer, 77 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MRNELEEMQRRADQLADESL ESTRRMLQLVEESKDAGIRT LVMLDEQGEQLDRVEEGMNH INQDMKEAEKNLKDLGK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	227
15N chemical shifts	77
1H chemical shifts	77

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SN1(7-83) of SNAP25	1

Entities:

Entity 1, SN1(7-83) of SNAP25 77 residues - Formula weight is not available

1

MET

ARG

ASN

GLU

LEU

GLU

MET

GLN

ARG

2

ARG

ALA

ASP

GLN

LEU

ALA

ASP

GLU

SER

LEU

3

GLU

SER

THR

ARG

MET

LEU

GLN

LEU

VAL

4

GLU

SER

LYS

ASP

ALA

GLY

ILE

ARG

THR

5

LEU

VAL

MET

LEU

ASP

GLU

GLN

GLY

GLU

GLN

6

LEU

ASP

ARG

VAL

GLU

GLY

MET

ASN

HIS

7

ILE

ASN

GLN

ASP

MET

LYS

GLU

ALA

GLU

LYS

8

ASN

LEU

LYS

ASP

LEU

GLY

LYS

Samples:

sample_1: HEPES 10 mM; MES 10 mM; sodium acetate 10 mM; sodium chloride 150 mM; SN1(7-83) of SNAP25, [U-99% 13C; U-99% 15N], 1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe - processing

SPARKY - data analysis

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks