BMRB Entry 5280
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5280
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Citation: Jayawardene, Dhammika; Liu, Junmin; Zhang, Weixing; Kriwacki, Richard. "Letter to the Editor: Assignments of the 1H, 13C, and 15N, resonances of the
winged helix domain of the proto-oncoprotein c-Qin (FoxG1B) " J. Biomol. NMR 23, 243-244 (2002).
Assembly members:
cellular Qin winged helix domain, polymer, 127 residues, 14720.8 Da.
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET_28a
Entity Sequences (FASTA):
cellular Qin winged helix domain: GSHMGKEGEKKNGKYEKPPF
SYNALIMMAIRQSPEKRLTL
NGIYEFIMKNFPYYRENKQG
WQNSIRHNLSLNKSFVKVPR
HYDDPGKGNYWMLDPSSDDV
FIGGTTGKLRRRSTTSRAKL
AFKRGAR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 863 |
| 13C chemical shifts | 548 |
| 15N chemical shifts | 133 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | c-Qin-WHD | 1 |
Entities:
Entity 1, c-Qin-WHD 127 residues - 14720.8 Da.
| 1 | GLY | SER | HIS | MET | GLY | LYS | GLU | GLY | GLU | LYS | ||||
| 2 | LYS | ASN | GLY | LYS | TYR | GLU | LYS | PRO | PRO | PHE | ||||
| 3 | SER | TYR | ASN | ALA | LEU | ILE | MET | MET | ALA | ILE | ||||
| 4 | ARG | GLN | SER | PRO | GLU | LYS | ARG | LEU | THR | LEU | ||||
| 5 | ASN | GLY | ILE | TYR | GLU | PHE | ILE | MET | LYS | ASN | ||||
| 6 | PHE | PRO | TYR | TYR | ARG | GLU | ASN | LYS | GLN | GLY | ||||
| 7 | TRP | GLN | ASN | SER | ILE | ARG | HIS | ASN | LEU | SER | ||||
| 8 | LEU | ASN | LYS | SER | PHE | VAL | LYS | VAL | PRO | ARG | ||||
| 9 | HIS | TYR | ASP | ASP | PRO | GLY | LYS | GLY | ASN | TYR | ||||
| 10 | TRP | MET | LEU | ASP | PRO | SER | SER | ASP | ASP | VAL | ||||
| 11 | PHE | ILE | GLY | GLY | THR | THR | GLY | LYS | LEU | ARG | ||||
| 12 | ARG | ARG | SER | THR | THR | SER | ARG | ALA | LYS | LEU | ||||
| 13 | ALA | PHE | LYS | ARG | GLY | ALA | ARG |
Samples:
15N_13C_labelled: cellular Qin winged helix domain, [U-98% 13C; U-98% 15N], 2.0 mM
15N_labelled: cellular Qin winged helix domain, [U-98% 15N], 2 mM
Cond_1: pH: 5.5; temperature: 313 K; ionic strength: 0.100 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| CBCA(CO)NH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CA)CO | not available | not available | not available |
| (H)C(CO)NH-TOCSY | not available | not available | not available |
| H(CCO)NH-TOCSY | not available | not available | not available |
| HBHA(CBCACO)NH | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| LRCC | not available | not available | not available |
| LRCH | not available | not available | not available |
| 15N-edited NOESY | not available | not available | not available |
| 13C-edited NOESY | not available | not available | not available |
| aromatic- HB(CBCG)CDHD | not available | not available | not available |
| (HB)CB(CG)CDHD | not available | not available | not available |
| HB(CBCECD)CEHE | not available | not available | not available |
| (HB)CB(CGCD)CEHE edited 3D NOESY | not available | not available | not available |
| (HB)CB(CGCD)CEHE edited 4D NOESY | not available | not available | not available |
Software:
Felix v98 - NMR data processing, analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks