BMRB Entry 52764

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52764

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Title:
Staufen dsRBD34
Deposition date:
2024-12-17
Original release date:
2025-05-30
Authors:
Heber, Simone; Simon, Bernd
Citation:

Citation: Heber, Simone. "A direct interaction between the RNA-binding proteins Staufen and Tm1-I/C regulates oskar mRNP composition and transport"  Cell Rep. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 376 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPLSPISQVHEIGIKRNMTV HFKVLREEGPAHMKNFITAC IVGSIVTEGEGNGKKVSKKR AAEKMLVELQKLPPLTPTKQ TPLKRIKVKTPGKSGAAARE GSVVSGTDGPTQTGKPERRK RLNPPKDKLIDMDDADNPIT KLIQLQQTRKEKEPIFELIA KNGNETARRREFVMEVSASG STARGTGNSKKLAKRNAAQA LFELLEAVQVTPTNETQSSE ECCTSATMSAVTAPAVEATA EGKVPMVATPVGPMPGILIL RQNKKPAKKRDQIVIVKSNV ESKEEEANKEVAVAAEENSN NSANSGDSSNSSSGDSQATE AASESALNTSTGSNTSGVSS NSSNVGANTDGNNHAESKNN TESSSNSTSNTQSAGV

Data sets:
Data typeCount
13C chemical shifts150
15N chemical shifts70
1H chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dsRBD341

Entities:

Entity 1, dsRBD34 376 residues - Formula weight is not available

1   GLYPROLEUSERPROILESERGLNVALHIS
2   GLUILEGLYILELYSARGASNMETTHRVAL
3   HISPHELYSVALLEUARGGLUGLUGLYPRO
4   ALAHISMETLYSASNPHEILETHRALACYS
5   ILEVALGLYSERILEVALTHRGLUGLYGLU
6   GLYASNGLYLYSLYSVALSERLYSLYSARG
7   ALAALAGLULYSMETLEUVALGLULEUGLN
8   LYSLEUPROPROLEUTHRPROTHRLYSGLN
9   THRPROLEULYSARGILELYSVALLYSTHR
10   PROGLYLYSSERGLYALAALAALAARGGLU
11   GLYSERVALVALSERGLYTHRASPGLYPRO
12   THRGLNTHRGLYLYSPROGLUARGARGLYS
13   ARGLEUASNPROPROLYSASPLYSLEUILE
14   ASPMETASPASPALAASPASNPROILETHR
15   LYSLEUILEGLNLEUGLNGLNTHRARGLYS
16   GLULYSGLUPROILEPHEGLULEUILEALA
17   LYSASNGLYASNGLUTHRALAARGARGARG
18   GLUPHEVALMETGLUVALSERALASERGLY
19   SERTHRALAARGGLYTHRGLYASNSERLYS
20   LYSLEUALALYSARGASNALAALAGLNALA
21   LEUPHEGLULEULEUGLUALAVALGLNVAL
22   THRPROTHRASNGLUTHRGLNSERSERGLU
23   GLUCYSCYSTHRSERALATHRMETSERALA
24   VALTHRALAPROALAVALGLUALATHRALA
25   GLUGLYLYSVALPROMETVALALATHRPRO
26   VALGLYPROMETPROGLYILELEUILELEU
27   ARGGLNASNLYSLYSPROALALYSLYSARG
28   ASPGLNILEVALILEVALLYSSERASNVAL
29   GLUSERLYSGLUGLUGLUALAASNLYSGLU
30   VALALAVALALAALAGLUGLUASNSERASN
31   ASNSERALAASNSERGLYASPSERSERASN
32   SERSERSERGLYASPSERGLNALATHRGLU
33   ALAALASERGLUSERALALEUASNTHRSER
34   THRGLYSERASNTHRSERGLYVALSERSER
35   ASNSERSERASNVALGLYALAASNTHRASP
36   GLYASNASNHISALAGLUSERLYSASNASN
37   THRGLUSERSERSERASNSERTHRSERASN
38   THRGLNSERALAGLYVAL

Samples:

sample_1: Staufen dsRBD34, [U-100% 13C; U-100% 15N], 200 ± 20 uM; sodium phosphate 25 mM; TCEP 0.5 mM; sodium chloride 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRView v4.1.3 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks