BMRB Entry 52735

Name: CrsL, a novel transcription factor in Mycobacterium smegmatis
Published: 2025-09-20

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52735

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CrsL, a novel transcription factor in Mycobacterium smegmatis

Deposition date:

2024-12-02

Original release date:

2025-09-20

Authors:

Cerny, Martin; Shoman, Mahmoud; Hnilicova, Jarmila; Zidek, Lukas

Citation:

Citation: Shoman, Mahmoud; Jirat Matejckova, Jitka; Schwarz, Marek; Cerny, Martin; Borah, Nabajyoti; Vankova Hausnerova, Viola; Sikova, Michaela; Sanderova, Hana; Halada, Petr; Hubalek, Martin; Dvorakova, Vera; Prevorovsky, Martin; Holubova, Jana; Stanek, Ondrej; Krasny, Libor; Zidek, Lukas; Hnilicova, Jarmila. "Expanding the CarD interaction network: CrsL is a novel transcription factor in Mycobacterium smegmatis" Nucleic Acids Res. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 56 residues, 5970 Da.

Natural source:

Natural source: Common Name: Mycobacterium smegmatis Taxonomy ID: 1772 Superkingdom: Bacteria Kingdom: not available Genus/species: Mycobacterium smegmatis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MPAKTDPADLGDVEPLADDT ASQARRVVATYATDADECRM FLSMLGIGPSKTEVLE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	162
15N chemical shifts	54
1H chemical shifts	51

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CrsL	1

Entities:

Entity 1, CrsL 56 residues - 5970 Da.

1

MET

PRO

ALA

LYS

THR

ASP

PRO

ALA

ASP

LEU

2

GLY

ASP

VAL

GLU

PRO

LEU

ALA

ASP

THR

3

ALA

SER

GLN

ALA

ARG

VAL

ALA

THR

4

TYR

ALA

THR

ASP

ALA

ASP

GLU

CYS

ARG

MET

5

PHE

LEU

SER

MET

LEU

GLY

ILE

GLY

PRO

SER

6

LYS

THR

GLU

VAL

LEU

GLU

Samples:

sample_1: CrsL, [U-100% 13C; U-100% 15N], 0.475 mM; sodium phosphate 20 mM; sodium chloride 10 mM; sodium azide 1 mM; TCEP 0.5 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 300.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY v1.470 - chemical shift assignment, peak picking

TOPSPIN v4.0.9, 4.1.4 - collection

NMRDraw v11.2 - processing

NMRPipe - processing

NMR spectrometers:

Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks