BMRB Entry 52713

Name: 1H, 15N, 13C backbone resonance assignment of full length apo human Alkbh7
Published: 2024-12-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52713

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C backbone resonance assignment of full length apo human Alkbh7

Deposition date:

2024-11-20

Original release date:

2024-12-05

Authors:

Faal, Baboucarr; Purslow, Jeffrey; Venditti, Vincenzo

Citation:

Citation: Faal, Baboucarr; Purslow, Jeffrey; Venditti, Vincenzo. "1H, 15N, 13C backbone resonance assignment of human Alkbh7" J. Biomol. NMR ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 221 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Modified pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAGTGLLALRTLPGPSWVRG SGPSVLSRLQDAAVVRPGFL STAEEETLSRELEPELRRRR YEYDHWDAAIHGFRETEKSR WSEASRAILQRVQAAAFGPG QTLLSSVHVLDLEARGYIKP HVDSIKFCGATIAGLSLLSP SVMRLVHTQEPGEWLELLLE PGSLYILRGSARYDFSHEIL RDEESFFGERRIPRGRRISV ICRSLPEGMGPGESGQPPPA C

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	462
15N chemical shifts	143
1H chemical shifts	143

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Alkbh7	1

Entities:

Entity 1, Alkbh7 221 residues - Formula weight is not available

1

MET

ALA

GLY

THR

GLY

LEU

ALA

LEU

ARG

2

THR

LEU

PRO

GLY

PRO

SER

TRP

VAL

ARG

GLY

3

SER

GLY

PRO

SER

VAL

LEU

SER

ARG

LEU

GLN

4

ASP

ALA

VAL

ARG

PRO

GLY

PHE

LEU

5

SER

THR

ALA

GLU

THR

LEU

SER

ARG

6

GLU

LEU

GLU

PRO

GLU

LEU

ARG

7

TYR

GLU

TYR

ASP

HIS

TRP

ASP

ALA

ILE

8

HIS

GLY

PHE

ARG

GLU

THR

GLU

LYS

SER

ARG

9

TRP

SER

GLU

ALA

SER

ARG

ALA

ILE

LEU

GLN

10

ARG

VAL

GLN

ALA

PHE

GLY

PRO

GLY

11

GLN

THR

LEU

SER

VAL

HIS

VAL

LEU

12

ASP

LEU

GLU

ALA

ARG

GLY

TYR

ILE

LYS

PRO

13

HIS

VAL

ASP

SER

ILE

LYS

PHE

CYS

GLY

ALA

14

THR

ILE

ALA

GLY

LEU

SER

LEU

SER

PRO

15

SER

VAL

MET

ARG

LEU

VAL

HIS

THR

GLN

GLU

16

PRO

GLY

GLU

TRP

LEU

GLU

LEU

GLU

17

PRO

GLY

SER

LEU

TYR

ILE

LEU

ARG

GLY

SER

18

ALA

ARG

TYR

ASP

PHE

SER

HIS

GLU

ILE

LEU

19

ARG

ASP

GLU

SER

PHE

GLY

GLU

ARG

20

ARG

ILE

PRO

ARG

GLY

ARG

ILE

SER

VAL

21

ILE

CYS

ARG

SER

LEU

PRO

GLU

GLY

MET

GLY

22

PRO

GLY

GLU

SER

GLY

GLN

PRO

ALA

23

CYS

Samples:

sample_1: Alkbh7, [U-13C; U-15N; U-2H], 200 uM; MES 20 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

MARS - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks