BMRB Entry 52708

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for apo Rev7
Published: 2024-11-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52708

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for apo Rev7

Deposition date:

2024-11-18

Original release date:

2024-11-29

Authors:

Bohm, Raphael; Muntener, Thomas; Hiller, Sebastian

Citation:

Citation: Sobkowiak, Katarzyna; Kohzaki, Masaoki; Bohm, Raphael; Mailler, Jonathan; Huber, Florian; Emamzadah, Soheila; Tropia, Laurence; Hiller, Sebastian; Halazonetis, Thanos. "REV7 functions with REV3 as a checkpoint protein delaying mitotic entry until DNA replication is completed" Cell Reports ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 211 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pT5T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MTTLTRQDLNSAQVVADVLS EFLEVAVHLILYVREVYPVG YFQKRKKYNVPVQMSCHPEL NQYIQDTLHCVKPLLEKNDV EKVVVVILDKEHRPVEKFVF EITQPPLLSINSDSLLSHVE QLLRAFILKISKVDKVLDHN PPGCTFTVLVHTREAATRNA EKKQVIKDFPWILADEQDVH MHDPRLIPLKTMTSDILKMQ LYVEERAHKNS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	299
15N chemical shifts	157
1H chemical shifts	157

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Rev7_apo	1

Entities:

Entity 1, Rev7_apo 211 residues - Formula weight is not available

1

MET

THR

LEU

THR

ARG

GLN

ASP

LEU

ASN

2

SER

ALA

GLN

VAL

ALA

ASP

VAL

LEU

SER

3

GLU

PHE

LEU

GLU

VAL

ALA

VAL

HIS

LEU

ILE

4

LEU

TYR

VAL

ARG

GLU

VAL

TYR

PRO

VAL

GLY

5

TYR

PHE

GLN

LYS

ARG

LYS

TYR

ASN

VAL

6

PRO

VAL

GLN

MET

SER

CYS

HIS

PRO

GLU

LEU

7

ASN

GLN

TYR

ILE

GLN

ASP

THR

LEU

HIS

CYS

8

VAL

LYS

PRO

LEU

GLU

LYS

ASN

ASP

VAL

9

GLU

LYS

VAL

ILE

LEU

ASP

LYS

10

GLU

HIS

ARG

PRO

VAL

GLU

LYS

PHE

VAL

PHE

11

GLU

ILE

THR

GLN

PRO

LEU

SER

ILE

12

ASN

SER

ASP

SER

LEU

SER

HIS

VAL

GLU

13

GLN

LEU

ARG

ALA

PHE

ILE

LEU

LYS

ILE

14

SER

LYS

VAL

ASP

LYS

VAL

LEU

ASP

HIS

ASN

15

PRO

GLY

CYS

THR

PHE

THR

VAL

LEU

VAL

16

HIS

THR

ARG

GLU

ALA

THR

ARG

ASN

ALA

17

GLU

LYS

GLN

VAL

ILE

LYS

ASP

PHE

PRO

18

TRP

ILE

LEU

ALA

ASP

GLU

GLN

ASP

VAL

HIS

19

MET

HIS

ASP

PRO

ARG

LEU

ILE

PRO

LEU

LYS

20

THR

MET

THR

SER

ASP

ILE

LEU

LYS

MET

GLN

21

LEU

TYR

VAL

GLU

ARG

ALA

HIS

LYS

ASN

22

SER

Samples:

sample_1: Rev7_apo, [U-13C; U-15N; U-2H], 311 uM; DTT 2 mM; NaCl 200 mM; EDTA 2 mM; Bis-Tris-Propane 25 mM

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks