BMRB Entry 52707
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52707
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Kohoutova, Klara; Srb, Pavel; Obsilova, Veronika; Veverka, Vaclav; Obsil, Tomas. "Structural Plasticity of the FOXO-DBD:p53-TAD Interaction" Nat. Commun. ., .-..
Assembly members:
entity_1, polymer, 93 residues, Formula weight is not available
entity_2, polymer, 126 residues, Formula weight is not available
entity_3, polymer, 17 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b
Entity Sequences (FASTA):
entity_1: MEEPQSDPSVEPPLSQETFS
DLWKLLPENNVLSPLPSQAM
DDLMLSPDDIEQWFTEDPGP
DEAPRMPEAAPPVAPAPAAP
TPAAPAPAPSWPL
entity_2: GAVTGPRKGGSRRNAWGNQS
YAELISQAIESAPEKRLTLA
QIYEWMVRTVPYFKDKGDSN
SSAGWKNSIRHNLSLHSKFI
KVHNEATGKSSWWMLNPEGG
KSGKAPRRRAASMDSSSKLL
RGRSKA
entity_3: GACTATGTAAACAACGC
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| T1 relaxation values | 64 |
| T2 relaxation values | 64 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p53(1-93) | 1 |
| 2 | FOXO4(86-211) | 2 |
| 3 | DNA | 3 |
Entities:
Entity 1, p53(1-93) 93 residues - Formula weight is not available
| 1 | MET | GLU | GLU | PRO | GLN | SER | ASP | PRO | SER | VAL | ||||
| 2 | GLU | PRO | PRO | LEU | SER | GLN | GLU | THR | PHE | SER | ||||
| 3 | ASP | LEU | TRP | LYS | LEU | LEU | PRO | GLU | ASN | ASN | ||||
| 4 | VAL | LEU | SER | PRO | LEU | PRO | SER | GLN | ALA | MET | ||||
| 5 | ASP | ASP | LEU | MET | LEU | SER | PRO | ASP | ASP | ILE | ||||
| 6 | GLU | GLN | TRP | PHE | THR | GLU | ASP | PRO | GLY | PRO | ||||
| 7 | ASP | GLU | ALA | PRO | ARG | MET | PRO | GLU | ALA | ALA | ||||
| 8 | PRO | PRO | VAL | ALA | PRO | ALA | PRO | ALA | ALA | PRO | ||||
| 9 | THR | PRO | ALA | ALA | PRO | ALA | PRO | ALA | PRO | SER | ||||
| 10 | TRP | PRO | LEU |
Entity 2, FOXO4(86-211) 126 residues - Formula weight is not available
| 1 | GLY | ALA | VAL | THR | GLY | PRO | ARG | LYS | GLY | GLY | ||||
| 2 | SER | ARG | ARG | ASN | ALA | TRP | GLY | ASN | GLN | SER | ||||
| 3 | TYR | ALA | GLU | LEU | ILE | SER | GLN | ALA | ILE | GLU | ||||
| 4 | SER | ALA | PRO | GLU | LYS | ARG | LEU | THR | LEU | ALA | ||||
| 5 | GLN | ILE | TYR | GLU | TRP | MET | VAL | ARG | THR | VAL | ||||
| 6 | PRO | TYR | PHE | LYS | ASP | LYS | GLY | ASP | SER | ASN | ||||
| 7 | SER | SER | ALA | GLY | TRP | LYS | ASN | SER | ILE | ARG | ||||
| 8 | HIS | ASN | LEU | SER | LEU | HIS | SER | LYS | PHE | ILE | ||||
| 9 | LYS | VAL | HIS | ASN | GLU | ALA | THR | GLY | LYS | SER | ||||
| 10 | SER | TRP | TRP | MET | LEU | ASN | PRO | GLU | GLY | GLY | ||||
| 11 | LYS | SER | GLY | LYS | ALA | PRO | ARG | ARG | ARG | ALA | ||||
| 12 | ALA | SER | MET | ASP | SER | SER | SER | LYS | LEU | LEU | ||||
| 13 | ARG | GLY | ARG | SER | LYS | ALA |
Entity 3, DNA 17 residues - Formula weight is not available
| 1 | DG | DA | DC | DT | DA | DT | DG | DT | DA | DA | ||||
| 2 | DA | DC | DA | DA | DC | DG | DC |
Samples:
sample_1: p53, [U-100% 15N], 100 uM; FOXO4(86-211) 200 uM; DNA 100 uM
sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| T1/R1 relaxation | sample_1 | isotropic | sample_conditions_1 |
| T2/R2 relaxation | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.6.2 - collection
NMRFAM-SPARKY v3.1 - data analysis
NMR spectrometers:
- Bruker AVANCE III 850 MHz