BMRB Entry 52697

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52697

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
Tau151-391 PAD12
Deposition date:
2024-11-13
Original release date:
2026-05-13
Authors:
Lovestam, Sofia; Wagstaff, Jane; Freund, Stefan; Scheres, Sjors
Citation:

Citation: Lovestam, Sofia; Wagstaff, Jane; Katinelos, Taxiarchis; Freund, Stefan; Goedert, Michel; Scheres, Sjors. "Twelve phoshomimetic mutations induce the assembly of recombinant full-length human tau into paired helical filaments"  elife ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 242 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRK172

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MIATPRGAAPPGQKGQANAT RIPAKTPPAPKDPPSSGEPP KSGDRSGYSSPGDPGDPGSR SRDPDLPDPPTREPKKVAVV RDPPKDPSSAKSRLQTAPVP MPDLKNVKSKIGSTENLKHQ PGGGKVQIINKKLDLSNVQS KCGSKDNIKHVPGGGSVQIV YKPVDLSKVTSKCGSLGNIH HKPGGGQVEVKSEKLDFKDR VQSKIGSLDNITHVPGGGNK KIETHKLTFRENAKAKTDHG AE

Data sets:
Data typeCount
13C chemical shifts624
15N chemical shifts206
1H chemical shifts173

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tau 151-391 PAD121

Entities:

Entity 1, Tau 151-391 PAD12 242 residues - Formula weight is not available

1   METILEALATHRPROARGGLYALAALAPRO
2   PROGLYGLNLYSGLYGLNALAASNALATHR
3   ARGILEPROALALYSTHRPROPROALAPRO
4   LYSASPPROPROSERSERGLYGLUPROPRO
5   LYSSERGLYASPARGSERGLYTYRSERSER
6   PROGLYASPPROGLYASPPROGLYSERARG
7   SERARGASPPROASPLEUPROASPPROPRO
8   THRARGGLUPROLYSLYSVALALAVALVAL
9   ARGASPPROPROLYSASPPROSERSERALA
10   LYSSERARGLEUGLNTHRALAPROVALPRO
11   METPROASPLEULYSASNVALLYSSERLYS
12   ILEGLYSERTHRGLUASNLEULYSHISGLN
13   PROGLYGLYGLYLYSVALGLNILEILEASN
14   LYSLYSLEUASPLEUSERASNVALGLNSER
15   LYSCYSGLYSERLYSASPASNILELYSHIS
16   VALPROGLYGLYGLYSERVALGLNILEVAL
17   TYRLYSPROVALASPLEUSERLYSVALTHR
18   SERLYSCYSGLYSERLEUGLYASNILEHIS
19   HISLYSPROGLYGLYGLYGLNVALGLUVAL
20   LYSSERGLULYSLEUASPPHELYSASPARG
21   VALGLNSERLYSILEGLYSERLEUASPASN
22   ILETHRHISVALPROGLYGLYGLYASNLYS
23   LYSILEGLUTHRHISLYSLEUTHRPHEARG
24   GLUASNALALYSALALYSTHRASPHISGLY
25   ALAGLU

Samples:

sample_1: Tau 151-391 PAD12, [U-98% 13C; U-98% 15N], 300 uM; D2O, [U-99% 2H], 5%; potassium phosphate 50 mM; sodium chloride 150 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOCANNHsample_1isotropicsample_conditions_1
3D C_HCACONsample_1isotropicsample_conditions_1
3D C_HCANCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.6.0 - collection

NMRFAM-SPARKY - peak picking

NMRFx Processor - processing

MARS - data analysis

NMR spectrometers:

  • Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks