BMRB Entry 5267

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5267

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N NMR sequencespecific resonance assignment of a Clostridium thermocellum type II cohesin module

Deposition date:

2002-01-28

Original release date:

2002-08-23

Authors:

Smith, Steven; Beguin, Pierre; Alzari, Pedro; Gehring, Kalle

Citation:

Citation: Smith, Steven; Beguin, Pierre; Alzari, Pedro; Gehring, Kalle. "Letter to the Editor: 1H, 13C, 15N NMR sequence-specific resonance assignment of a Clostridium thermocellum type II cohesin module " J. Biomol. NMR 23, 73-74 (2002).
PubMed: 12061722

Assembly members:

Assembly members:
SdbA type II cohesin, polymer, 187 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Clostridium thermocellum Taxonomy ID: 1515 Superkingdom: Eubacteria Kingdom: not available Genus/species: Clostridium thermocellum

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SdbA type II cohesin: MRGSHHHHHHTDLRADKASS IELKFDRNKGEVGDILIGTV RINNIKNFAGFQVNIVYDPK VLMAVDPETGKEFTSSTFPP GRTVLKNNAYGPIQIADNDP EKGILNFALAYSYIAGYKET GVAEESGIIAKIGFKILQKK STAVKFQDTLSMPGAISGTQ LFDWDGEVITGYEVIQPDVL SLGDEPF

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	966
13C chemical shifts	650
15N chemical shifts	162

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SdbA cohesin module	1

Entities:

Entity 1, SdbA cohesin module 187 residues - Formula weight is not available

1

MET

ARG

GLY

SER

HIS

2

THR

ASP

LEU

ARG

ALA

ASP

LYS

ALA

SER

3

ILE

GLU

LEU

LYS

PHE

ASP

ARG

ASN

LYS

GLY

4

GLU

VAL

GLY

ASP

ILE

LEU

ILE

GLY

THR

VAL

5

ARG

ILE

ASN

ILE

LYS

ASN

PHE

ALA

GLY

6

PHE

GLN

VAL

ASN

ILE

VAL

TYR

ASP

PRO

LYS

7

VAL

LEU

MET

ALA

VAL

ASP

PRO

GLU

THR

GLY

8

LYS

GLU

PHE

THR

SER

THR

PHE

PRO

9

GLY

ARG

THR

VAL

LEU

LYS

ASN

ALA

TYR

10

GLY

PRO

ILE

GLN

ILE

ALA

ASP

ASN

ASP

PRO

11

GLU

LYS

GLY

ILE

LEU

ASN

PHE

ALA

LEU

ALA

12

TYR

SER

TYR

ILE

ALA

GLY

TYR

LYS

GLU

THR

13

GLY

VAL

ALA

GLU

SER

GLY

ILE

ALA

14

LYS

ILE

GLY

PHE

LYS

ILE

LEU

GLN

LYS

15

SER

THR

ALA

VAL

LYS

PHE

GLN

ASP

THR

LEU

16

SER

MET

PRO

GLY

ALA

ILE

SER

GLY

THR

GLN

17

LEU

PHE

ASP

TRP

ASP

GLY

GLU

VAL

ILE

THR

18

GLY

TYR

GLU

VAL

ILE

GLN

PRO

ASP

VAL

LEU

19

SER

LEU

GLY

ASP

GLU

PRO

PHE

Samples:

sample_1: SdbA type II cohesin, [U-95% 13C; U-90% 15N], 1.2 mM

sample_2: SdbA type II cohesin, [U-90% 15N], 1.2 mM

Excond_1: pH: 6.9 na; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H15N HSQC	not available	not available	not available
1H 13C HSQC	not available	not available	not available
3D HNHA	not available	not available	not available
3D HNCO	not available	not available	not available
3D HNCACB	not available	not available	not available
3D CBCA(CO)NH	not available	not available	not available
15N NOESY HSQC	not available	not available	not available
CC(CO)NH TOCSY	not available	not available	not available
HCC(CO)NH TOCSY	not available	not available	not available
(H)CCH COSY	not available	not available	not available
HCCH TOCSY	not available	not available	not available

Software:

XEASY - peak assignment

NMR spectrometers:

Bruker AVANCE 600 MHz
Bruker DRX 500 MHz

PDB	2B59 2BM3 3KCP 4FL4
EMBL	CDG35978
GB	AAB07763 ABN52539 ADU74018 EEU01806 EFB39640
REF	WP_003516982 WP_004463692 YP_001037732 YP_005687469