BMRB Entry 5267

Title:
1H, 13C, 15N NMR sequencespecific resonance assignment of a Clostridium thermocellum type II cohesin module
Deposition date:
2002-01-28
Original release date:
2002-08-23
Authors:
Smith, Steven; Beguin, Pierre; Alzari, Pedro; Gehring, Kalle
Citation:

Citation: Smith, Steven; Beguin, Pierre; Alzari, Pedro; Gehring, Kalle. "Letter to the Editor: 1H, 13C, 15N NMR sequence-specific resonance assignment of a Clostridium thermocellum type II cohesin module "  J. Biomol. NMR 23, 73-74 (2002).
PubMed: 12061722

Assembly members:

Assembly members:
SdbA type II cohesin, polymer, 187 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Clostridium thermocellum   Taxonomy ID: 1515   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Clostridium thermocellum

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts966
13C chemical shifts650
15N chemical shifts162

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SdbA cohesin module1

Entities:

Entity 1, SdbA cohesin module 187 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   THRASPLEUARGALAASPLYSALASERSER
3   ILEGLULEULYSPHEASPARGASNLYSGLY
4   GLUVALGLYASPILELEUILEGLYTHRVAL
5   ARGILEASNASNILELYSASNPHEALAGLY
6   PHEGLNVALASNILEVALTYRASPPROLYS
7   VALLEUMETALAVALASPPROGLUTHRGLY
8   LYSGLUPHETHRSERSERTHRPHEPROPRO
9   GLYARGTHRVALLEULYSASNASNALATYR
10   GLYPROILEGLNILEALAASPASNASPPRO
11   GLULYSGLYILELEUASNPHEALALEUALA
12   TYRSERTYRILEALAGLYTYRLYSGLUTHR
13   GLYVALALAGLUGLUSERGLYILEILEALA
14   LYSILEGLYPHELYSILELEUGLNLYSLYS
15   SERTHRALAVALLYSPHEGLNASPTHRLEU
16   SERMETPROGLYALAILESERGLYTHRGLN
17   LEUPHEASPTRPASPGLYGLUVALILETHR
18   GLYTYRGLUVALILEGLNPROASPVALLEU
19   SERLEUGLYASPGLUPROPHE

Samples:

sample_1: SdbA type II cohesin, [U-95% 13C; U-90% 15N], 1.2 mM

sample_2: SdbA type II cohesin, [U-90% 15N], 1.2 mM

Excond_1: pH: 6.9 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H15N HSQCnot availablenot availablenot available
1H 13C HSQCnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
15N NOESY HSQCnot availablenot availablenot available
CC(CO)NH TOCSYnot availablenot availablenot available
HCC(CO)NH TOCSYnot availablenot availablenot available
(H)CCH COSYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available

Software:

XEASY - peak assignment

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
EMBL CDG35978
GB AAB07763 ABN52539 ADU74018 EEU01806 EFB39640
REF WP_003516982 WP_004463692 YP_001037732 YP_005687469

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks