BMRB Entry 52669

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52669

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Title:
FUS LCRGG1 dispersed phase 1H-15N backbone relaxation
Deposition date:
2024-10-31
Original release date:
2025-01-31
Authors:
Wake, Noah
Citation:

Citation: Wake, Noah; Weng, Shuo-Lin; Zheng, Tongyin; Wang, Szu-Huan; Kirilenko, Valentin; Mittal, Jeetain; Fawzi, Nicolas. "Expanding the molecular grammar of polar residues and arginine in FUS prion-like domain phase separation and aggregation."  bioRxiv ., .-. (2024).
PubMed: 38405719

Assembly members:

Assembly members:
entity_1, polymer, 284 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: RP1B_FUS_1-284_WT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MASNDYTQQATQSYGAYPTQ PGQGYSQQSSQPYGQQSYSG YSQSTDTSGYGQSSYSSYGQ SQNTGYGTQSTPQGYGSTGG YGSSQSSQSSYGQQSSYPGY GQQPAPSSTSGSYGSSSQSS SYGQPQSGSYSQQPSYGGQQ QSYGQQQSYNPPQGYGQQNQ YNSSSGGGGGGGGGGNYGQD QSSMSSGGGSGGGYGNQDQS GGGGSGGYGQQDRGGRGRGG SGGGGGGGGGGYNRSSGGYE PRGRGGGRGGRGGMGGSDRG GFNKFGGPRDQGSRHDSEQD NSDN

Data sets:
Data typeCount
T1 relaxation values208
T2 relaxation values208
heteronuclear NOE values208

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Fused in Sarcoma1

Entities:

Entity 1, Fused in Sarcoma 284 residues - Formula weight is not available

1   METALASERASNASPTYRTHRGLNGLNALA
2   THRGLNSERTYRGLYALATYRPROTHRGLN
3   PROGLYGLNGLYTYRSERGLNGLNSERSER
4   GLNPROTYRGLYGLNGLNSERTYRSERGLY
5   TYRSERGLNSERTHRASPTHRSERGLYTYR
6   GLYGLNSERSERTYRSERSERTYRGLYGLN
7   SERGLNASNTHRGLYTYRGLYTHRGLNSER
8   THRPROGLNGLYTYRGLYSERTHRGLYGLY
9   TYRGLYSERSERGLNSERSERGLNSERSER
10   TYRGLYGLNGLNSERSERTYRPROGLYTYR
11   GLYGLNGLNPROALAPROSERSERTHRSER
12   GLYSERTYRGLYSERSERSERGLNSERSER
13   SERTYRGLYGLNPROGLNSERGLYSERTYR
14   SERGLNGLNPROSERTYRGLYGLYGLNGLN
15   GLNSERTYRGLYGLNGLNGLNSERTYRASN
16   PROPROGLNGLYTYRGLYGLNGLNASNGLN
17   TYRASNSERSERSERGLYGLYGLYGLYGLY
18   GLYGLYGLYGLYGLYASNTYRGLYGLNASP
19   GLNSERSERMETSERSERGLYGLYGLYSER
20   GLYGLYGLYTYRGLYASNGLNASPGLNSER
21   GLYGLYGLYGLYSERGLYGLYTYRGLYGLN
22   GLNASPARGGLYGLYARGGLYARGGLYGLY
23   SERGLYGLYGLYGLYGLYGLYGLYGLYGLY
24   GLYTYRASNARGSERSERGLYGLYTYRGLU
25   PROARGGLYARGGLYGLYGLYARGGLYGLY
26   ARGGLYGLYMETGLYGLYSERASPARGGLY
27   GLYPHEASNLYSPHEGLYGLYPROARGASP
28   GLNGLYSERARGHISASPSERGLUGLNASP
29   ASNSERASPASN

Samples:

sample_1: MES 20 mM; sodium chloride 150 mM; D2O, [U-2H], 5 % v/v; FUS LCRGG1, [U-100% 15N], 10 uM

sample_conditions_1: ionic strength: 150 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
T1/R1 relaxationsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1
1H-15N heteronoesample_1isotropicsample_conditions_1

Software:

CcpNMR - data analysis

TOPSPIN - collection

NMR spectrometers:

  • Bruker AVANCE III HD 850 MHz