BMRB Entry 52649

Name: mouse cGAS N-terminal domain
Published: 2024-10-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52649

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

mouse cGAS N-terminal domain

Deposition date:

2024-10-22

Original release date:

2024-10-29

Authors:

Aucharova, Hanna; Linser, Rasmus

Citation:

Citation: Kavaleuskaya, Hanna; Linser, Rasmus. "1H, 15N, 13C Assigned Chemical Shifts for mouse cGAS N-terminal domain" Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 147 residues, 15938.84 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pOPINE-EGFP-3C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GPEDPRRRTTAPRAKKPSAK RAPTQPSRTRAHAESCGPQR GARSRRAERDGDTTEKPRAP GPRVHPARATELTKDAQPSA MDAAGATARPAVRVPQQQAI LDPELPAVREPQPPADPEAR KVVRGPSHRRGARSTGQPRA RRGSRKE

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1
spectral_peak_list

Data type	Count
13C chemical shifts	385
15N chemical shifts	113
1H chemical shifts	113

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 147 residues - 15938.84 Da.

1

GLY

PRO

GLU

ASP

PRO

ARG

THR

2

ALA

PRO

ARG

ALA

LYS

PRO

SER

ALA

LYS

3

ARG

ALA

PRO

THR

GLN

PRO

SER

ARG

THR

ARG

4

ALA

HIS

ALA

GLU

SER

CYS

GLY

PRO

GLN

ARG

5

GLY

ALA

ARG

SER

ARG

ALA

GLU

ARG

ASP

6

GLY

ASP

THR

GLU

LYS

PRO

ARG

ALA

PRO

7

GLY

PRO

ARG

VAL

HIS

PRO

ALA

ARG

ALA

THR

8

GLU

LEU

THR

LYS

ASP

ALA

GLN

PRO

SER

ALA

9

MET

ASP

ALA

GLY

ALA

THR

ALA

ARG

PRO

10

ALA

VAL

ARG

VAL

PRO

GLN

ALA

ILE

11

LEU

ASP

PRO

GLU

LEU

PRO

ALA

VAL

ARG

GLU

12

PRO

GLN

PRO

ALA

ASP

PRO

GLU

ALA

ARG

13

LYS

VAL

ARG

GLY

PRO

SER

HIS

ARG

14

GLY

ALA

ARG

SER

THR

GLY

GLN

PRO

ARG

ALA

15

PRO

ARG

GLY

SER

ARG

LYS

GLU

Samples:

sample_1: D2O, [U-100% 2H], 10%; DSS 1 mM; sodium chloride 0.1 M; MES 20 mM; TCEP 5 mM; mouse cGAS NTD (1-147), [U-100% 13C; U-100% 15N], 1 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCANH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
4D HN(COCA)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR v3.2.2 - chemical shift assignment

TOPSPIN v4.1.4 - processing

NMR spectrometers:

Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks