BMRB Entry 52647

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52647

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Title:
complex DdvANt with DdvS sigma 2 domain
Deposition date:
2024-10-21
Original release date:
2025-01-09
Authors:
Pantoja-Uceda, D.; Padmanabhan, S.
Citation:

Citation: Bernal-Bernal, Diego; Pantoja-Uceda, David; Lopez-Alonso, Jorge Pedro; Lopez-Rojo, Alfonso; Lopez-Ruiz, Jose Antonio; Galbis-Martinez, Marisa; Ochoa-Lizarralde, Borja; Tascon, Igor; Elias-Arnanz, Montserrat; Ubarretxena-Belandia, Iban; Padmanabhan, S.. "Structural basis for regulation of a CBASS-CRISPR-Cas defense island by a transmembrane anti-sigma factor and its ECF sigma partner"  Sci. Adv. 10, eadp1053-eadp1053 (2024).
PubMed: 39454004

Assembly members:

Assembly members:
entity_1, polymer, 90 residues, 10068.254 Da.
entity_2, polymer, 92 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Myxococcus xanthus   Taxonomy ID: 34   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Myxococcus xanthus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pKT25

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSSHHHHHHSSGLVPRGSHM MSSSPCDQLQSFADGDLPPM EAQAFGQHLADCEKCQVELT RLLQLDQLGRGYIERHGPVD IPWHALPRNR
entity_2: MTSAGETVASNFDEWHERYY GRLMPTAEKLCRGAVGIDPK DLVQETLLRFIEHYEAAPSE PKDGPAEGWLIKVMTHHFYD LIRGAMGRKKAE

Data sets:
Data typeCount
13C chemical shifts186
15N chemical shifts60
1H chemical shifts77

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22
3unit_33

Entities:

Entity 1, unit_1 90 residues - 10068.254 Da.

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUVALPROARGGLYSERHISMET
3   METSERSERSERPROCYSASPGLNLEUGLN
4   SERPHEALAASPGLYASPLEUPROPROMET
5   GLUALAGLNALAPHEGLYGLNHISLEUALA
6   ASPCYSGLULYSCYSGLNVALGLULEUTHR
7   ARGLEULEUGLNLEUASPGLNLEUGLYARG
8   GLYTYRILEGLUARGHISGLYPROVALASP
9   ILEPROTRPHISALALEUPROARGASNARG

Entity 2, unit_2 92 residues - Formula weight is not available

1   METTHRSERALAGLYGLUTHRVALALASER
2   ASNPHEASPGLUTRPHISGLUARGTYRTYR
3   GLYARGLEUMETPROTHRALAGLULYSLEU
4   CYSARGGLYALAVALGLYILEASPPROLYS
5   ASPLEUVALGLNGLUTHRLEULEUARGPHE
6   ILEGLUHISTYRGLUALAALAPROSERGLU
7   PROLYSASPGLYPROALAGLUGLYTRPLEU
8   ILELYSVALMETTHRHISHISPHETYRASP
9   LEUILEARGGLYALAMETGLYARGLYSLYS
10   ALAGLU

Entity 3, unit_3 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: DdvANt, [U-13C; U-15N], 0.7 mM; sodium chloride 100 mM; TRIS 20 mM; beta-mercaptoethanol 2 mM; sodium azide 0.05%; DdvS_sigma2 0.7 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQC BEST TROSYsample_1isotropicsample_conditions_1
3D HNCO BEST TROSYsample_1isotropicsample_conditions_1
3D HNCA BEST TROSYsample_1isotropicsample_conditions_1
3D CBCA(CO) BEST TROSYNHsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection, processing

NMRPipe - processing

NMRView - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks