BMRB Entry 52638
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52638
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Sroge, Collin; Suk, Jaewon; Padilla, Maria Sophia Teresa Lee; Zhu, Jason; Butts, Carter; Martin, Rachel. "Wild-Type mini-alphaA crystallin" .
Assembly members:
entity_1, polymer, 19 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: KFVIFLDVKHFSPEDLTVK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 119 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | WT MAAC | 1 |
Entities:
Entity 1, WT MAAC 19 residues - Formula weight is not available
| 1 | LYS | PHE | VAL | ILE | PHE | LEU | ASP | VAL | LYS | HIS | ||||
| 2 | PHE | SER | PRO | GLU | ASP | LEU | THR | VAL | LYS |
Samples:
sample_1: WT mini-alphaA crystallin 975 uM; D2O 10%; DSS 200 uM; sodium phosphate 20 mM
sample_2: WT mini-alphaA crystallin 300 uM; D2O 10%; DSS 200 uM; sodium phosphate 20 mM
sample_3: WT mini-alphaA crystallin 300 uM
sample_conditions_1: pH: 7.01; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
- Bruker Avance 600 MHz