BMRB Entry 52637

Name: Z-form DNA-RNA hybrid
Published: 2024-11-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52637

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Z-form DNA-RNA hybrid

Deposition date:

2024-10-09

Original release date:

2024-11-11

Authors:

Wang, Shiyu

Citation:

Citation: Wang, Shiyu. "Z-form DNA-RNA hybrid blocks DNA replication" Nucleic Acids Res. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 8 residues, Formula weight is not available
entity_2, polymer, 8 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XGXAXGXG
entity_2: CGCGUGCG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	141

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DNA chain	1
2	RNA chain	2

Entities:

Entity 1, DNA chain 8 residues - Formula weight is not available

1

5MC

DG

5MC

DA

5MC

DG

5MC

DG

Entity 2, RNA chain 8 residues - Formula weight is not available

1

C

G

C

G

U

G

C

G

Samples:

sample_1: DNA chain 5 mM; RNA chain 5 mM; KCl 100 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

MestReNova - chemical shift assignment

NMR spectrometers:

Bruker Avance 400 MHz