BMRB Entry 52630
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52630
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Gibbs, Eric; Miao, Qi; Ferrolino, Mylene; Bajpai, Richa; Hassan, Aila; Phillips, Aaron; Pitre, Aaron; Kummerle, Rainer; Miller, Shondra; Nagy, Gergely; Leite, Wellington; Heller, William; Stanley, Chris; Perrone, Barbara; Kriwacki, Richard. "p14ARF forms meso-scale assemblies upon phase separation with NPM1" Nat. Commun. 15, 9531-9531 (2024).
PubMed: 39528457
Assembly members:
entity_1, polymer, 296 residues, Formula weight is not available
entity_2, polymer, 154 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
entity_1: GSMEDSMDMDMSPLRPQNYL
FGCELKADKDYHFKVDNDEN
EHQLSLRTVSLGAGAKDELH
IVEAEAMNYEGSPIKVTLAT
LKMSVQPTVSLGGFEITPPV
VLRLKCGSGPVHISGQHLVA
VEEDAESEDEEEEDVKLLSI
SGKRSAPGGGSKVPQKKVKL
AADEDDDDDDEEDDDEDDDD
DDFDDEEAEEKAPVKKSIRD
TPAKNAQKSNQNGKDSKPSS
TPRSKGQESFKKQEKTPKTP
KGPSSVEDIKAKMQASIEKG
GSLPKVEAKFINYVKNCFRM
TDQEAIQDLWQWRKSL
entity_2: MGSSHHHHHHSQDLENLYFQ
GSMVRRFLVTLRIRRACGPP
RVRVFVVHIPRLTGEWAAPG
APAAVALVLMLLRSQRLGQQ
PLPRRPGHDDGQRPSGGAAA
APRRGAQLRRPRHSHPTRAR
RCPGGLPGHAGGAAPGRGAA
GRARCLGPSARGPG
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 30 |
| 1H chemical shifts | 30 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NPM1 | 1 |
| 2 | p14ARF | 2 |
Entities:
Entity 1, NPM1 296 residues - Formula weight is not available
| 1 | GLY | SER | MET | GLU | ASP | SER | MET | ASP | MET | ASP | ||||
| 2 | MET | SER | PRO | LEU | ARG | PRO | GLN | ASN | TYR | LEU | ||||
| 3 | PHE | GLY | CYS | GLU | LEU | LYS | ALA | ASP | LYS | ASP | ||||
| 4 | TYR | HIS | PHE | LYS | VAL | ASP | ASN | ASP | GLU | ASN | ||||
| 5 | GLU | HIS | GLN | LEU | SER | LEU | ARG | THR | VAL | SER | ||||
| 6 | LEU | GLY | ALA | GLY | ALA | LYS | ASP | GLU | LEU | HIS | ||||
| 7 | ILE | VAL | GLU | ALA | GLU | ALA | MET | ASN | TYR | GLU | ||||
| 8 | GLY | SER | PRO | ILE | LYS | VAL | THR | LEU | ALA | THR | ||||
| 9 | LEU | LYS | MET | SER | VAL | GLN | PRO | THR | VAL | SER | ||||
| 10 | LEU | GLY | GLY | PHE | GLU | ILE | THR | PRO | PRO | VAL | ||||
| 11 | VAL | LEU | ARG | LEU | LYS | CYS | GLY | SER | GLY | PRO | ||||
| 12 | VAL | HIS | ILE | SER | GLY | GLN | HIS | LEU | VAL | ALA | ||||
| 13 | VAL | GLU | GLU | ASP | ALA | GLU | SER | GLU | ASP | GLU | ||||
| 14 | GLU | GLU | GLU | ASP | VAL | LYS | LEU | LEU | SER | ILE | ||||
| 15 | SER | GLY | LYS | ARG | SER | ALA | PRO | GLY | GLY | GLY | ||||
| 16 | SER | LYS | VAL | PRO | GLN | LYS | LYS | VAL | LYS | LEU | ||||
| 17 | ALA | ALA | ASP | GLU | ASP | ASP | ASP | ASP | ASP | ASP | ||||
| 18 | GLU | GLU | ASP | ASP | ASP | GLU | ASP | ASP | ASP | ASP | ||||
| 19 | ASP | ASP | PHE | ASP | ASP | GLU | GLU | ALA | GLU | GLU | ||||
| 20 | LYS | ALA | PRO | VAL | LYS | LYS | SER | ILE | ARG | ASP | ||||
| 21 | THR | PRO | ALA | LYS | ASN | ALA | GLN | LYS | SER | ASN | ||||
| 22 | GLN | ASN | GLY | LYS | ASP | SER | LYS | PRO | SER | SER | ||||
| 23 | THR | PRO | ARG | SER | LYS | GLY | GLN | GLU | SER | PHE | ||||
| 24 | LYS | LYS | GLN | GLU | LYS | THR | PRO | LYS | THR | PRO | ||||
| 25 | LYS | GLY | PRO | SER | SER | VAL | GLU | ASP | ILE | LYS | ||||
| 26 | ALA | LYS | MET | GLN | ALA | SER | ILE | GLU | LYS | GLY | ||||
| 27 | GLY | SER | LEU | PRO | LYS | VAL | GLU | ALA | LYS | PHE | ||||
| 28 | ILE | ASN | TYR | VAL | LYS | ASN | CYS | PHE | ARG | MET | ||||
| 29 | THR | ASP | GLN | GLU | ALA | ILE | GLN | ASP | LEU | TRP | ||||
| 30 | GLN | TRP | ARG | LYS | SER | LEU |
Entity 2, p14ARF 154 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | GLN | ASP | LEU | GLU | ASN | LEU | TYR | PHE | GLN | ||||
| 3 | GLY | SER | MET | VAL | ARG | ARG | PHE | LEU | VAL | THR | ||||
| 4 | LEU | ARG | ILE | ARG | ARG | ALA | CYS | GLY | PRO | PRO | ||||
| 5 | ARG | VAL | ARG | VAL | PHE | VAL | VAL | HIS | ILE | PRO | ||||
| 6 | ARG | LEU | THR | GLY | GLU | TRP | ALA | ALA | PRO | GLY | ||||
| 7 | ALA | PRO | ALA | ALA | VAL | ALA | LEU | VAL | LEU | MET | ||||
| 8 | LEU | LEU | ARG | SER | GLN | ARG | LEU | GLY | GLN | GLN | ||||
| 9 | PRO | LEU | PRO | ARG | ARG | PRO | GLY | HIS | ASP | ASP | ||||
| 10 | GLY | GLN | ARG | PRO | SER | GLY | GLY | ALA | ALA | ALA | ||||
| 11 | ALA | PRO | ARG | ARG | GLY | ALA | GLN | LEU | ARG | ARG | ||||
| 12 | PRO | ARG | HIS | SER | HIS | PRO | THR | ARG | ALA | ARG | ||||
| 13 | ARG | CYS | PRO | GLY | GLY | LEU | PRO | GLY | HIS | ALA | ||||
| 14 | GLY | GLY | ALA | ALA | PRO | GLY | ARG | GLY | ALA | ALA | ||||
| 15 | GLY | ARG | ALA | ARG | CYS | LEU | GLY | PRO | SER | ALA | ||||
| 16 | ARG | GLY | PRO | GLY |
Samples:
sample_1: NPM1, [U-100% 13C; U-100% 15N], 1 mM; p14ARF 200 uM
sample_conditions_1: pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks