BMRB Entry 5263

Name: Backbone 1H, 13C, 15N chemical Shift assignments fo the CBM28
Published: 2002-08-22

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5263

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, 15N chemical Shift assignments fo the CBM28

Deposition date:

2002-01-21

Original release date:

2002-08-22

Authors:

Mosbah, Amor; Tardif, Chantal; Bornet, Olivier; Valette, Odile; Henrissat, Bernard; Darbon, Herve

Citation:

Citation: Mosbah, Amor; Tardif, Chantal; Bornet, Olivier; Valette, Odile; Henrissat, Bernard; Darbon, Herve. "Letter to the Editor: Assignment of the 1H, 13C, and 15N resonances of the 22,5 kDa CBM28 module of the cellulase Cel5I of Clostridium cellulolyticum" J. Biomol. NMR 23, 157-158 (2002).

Assembly members:

Assembly members:
CBM28 of CelI, polymer, 204 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Clostridium cellulolyticum Taxonomy ID: 1521 Superkingdom: Eubacteria Kingdom: not available Genus/species: Clostridium cellulolyticum

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CBM28 of CelI: MTTVEAPVEHAPIGKATLPS IFEDSTRQGWAWDAASGVQS ALTIKDANGSKAISWEVKYP EVKPVDGWASAPRIMLSNIN ATRGNNKYLAFDFYLKPTQA SKGSLSINLAFAPPTLGYWA QASVNFDIPLTKLSKMKKTK DGLYHFQVKYDLDKVNDGKV LAADTVLRDITIVVADGNSD FAGTMYVDNVRFENDSLEHH HHHH

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	318
15N chemical shifts	175
1H chemical shifts	646

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CBM28	1

Entities:

Entity 1, CBM28 204 residues - Formula weight is not available

1

MET

THR

VAL

GLU

ALA

PRO

VAL

GLU

HIS

2

ALA

PRO

ILE

GLY

LYS

ALA

THR

LEU

PRO

SER

3

ILE

PHE

GLU

ASP

SER

THR

ARG

GLN

GLY

TRP

4

ALA

TRP

ASP

ALA

SER

GLY

VAL

GLN

SER

5

ALA

LEU

THR

ILE

LYS

ASP

ALA

ASN

GLY

SER

6

LYS

ALA

ILE

SER

TRP

GLU

VAL

LYS

TYR

PRO

7

GLU

VAL

LYS

PRO

VAL

ASP

GLY

TRP

ALA

SER

8

ALA

PRO

ARG

ILE

MET

LEU

SER

ASN

ILE

ASN

9

ALA

THR

ARG

GLY

ASN

LYS

TYR

LEU

ALA

10

PHE

ASP

PHE

TYR

LEU

LYS

PRO

THR

GLN

ALA

11

SER

LYS

GLY

SER

LEU

SER

ILE

ASN

LEU

ALA

12

PHE

ALA

PRO

THR

LEU

GLY

TYR

TRP

ALA

13

GLN

ALA

SER

VAL

ASN

PHE

ASP

ILE

PRO

LEU

14

THR

LYS

LEU

SER

LYS

MET

LYS

THR

LYS

15

ASP

GLY

LEU

TYR

HIS

PHE

GLN

VAL

LYS

TYR

16

ASP

LEU

ASP

LYS

VAL

ASN

ASP

GLY

LYS

VAL

17

LEU

ALA

ASP

THR

VAL

LEU

ARG

ASP

ILE

18

THR

ILE

VAL

ALA

ASP

GLY

ASN

SER

ASP

19

PHE

ALA

GLY

THR

MET

TYR

VAL

ASP

ASN

VAL

20

ARG

PHE

GLU

ASN

ASP

SER

LEU

GLU

HIS

21

HIS

Samples:

sample_1: CBM28 of CelI mM

condition_set_1: pD: 5.0; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_1	not available	condition_set_1

Software:

No software information available

NMR spectrometers:

unknown unknown 0 MHz

GB	AAL79562 ACL74811
REF	WP_012634873 YP_002504791