BMRB Entry 52623

Name: Backbone resonance assignment for the N-terminal region of Zika virus NS4B in detergent micelles
Published: 2025-01-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52623

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignment for the N-terminal region of Zika virus NS4B in detergent micelles

Deposition date:

2024-10-01

Original release date:

2025-01-09

Authors:

Li, Yan; Loh, Ying Ru; Kang, Congbao

Citation:

Citation: Li, Yan; Loh, Ying Ru; Li, Qingxin; Luo, Dahai; Kang, CongBao. "1H, 15N and 13C backbone resonance assignment of the N-terminal region of Zika virus NS4B protein in detergent micelles" Biomol. NMR Assignments ., .-. (2024).
PubMed: 39505821

Assembly members:

Assembly members:
entity_1, polymer, 160 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Zika virus Taxonomy ID: 64320 Superkingdom: Viruses Kingdom: not available Genus/species: Orthoflavivirus zikaense

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MNELGWLERTKSDLSHLMGR REEGATIGFSMDIDLRPASA WAIYAALTTFITPAVQHAVT TSYNNYSLMAMATQAGVLFG MGKGMPFYAWDFGVPLLMIG CYSQLTPLTLIVAIILLVAH YMYLIPGLQAAAARAAQKRT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	349
15N chemical shifts	121
1H chemical shifts	121

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NS4B	1

Entities:

Entity 1, NS4B 160 residues - Formula weight is not available

MGSSHHHHHHSSGLVPRGSHM, is fusion tag, which is not assigned.

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	ASN	GLU	LEU	GLY	TRP	LEU	GLU	ARG	THR
4	LYS	SER	ASP	LEU	SER	HIS	LEU	MET	GLY	ARG
5	ARG	GLU	GLU	GLY	ALA	THR	ILE	GLY	PHE	SER
6	MET	ASP	ILE	ASP	LEU	ARG	PRO	ALA	SER	ALA
7	TRP	ALA	ILE	TYR	ALA	ALA	LEU	THR	THR	PHE
8	ILE	THR	PRO	ALA	VAL	GLN	HIS	ALA	VAL	THR
9	THR	SER	TYR	ASN	ASN	TYR	SER	LEU	MET	ALA
10	MET	ALA	THR	GLN	ALA	GLY	VAL	LEU	PHE	GLY
11	MET	GLY	LYS	GLY	MET	PRO	PHE	TYR	ALA	TRP
12	ASP	PHE	GLY	VAL	PRO	LEU	LEU	MET	ILE	GLY
13	CYS	TYR	SER	GLN	LEU	THR	PRO	LEU	THR	LEU
14	ILE	VAL	ALA	ILE	ILE	LEU	LEU	VAL	ALA	HIS
15	TYR	MET	TYR	LEU	ILE	PRO	GLY	LEU	GLN	ALA
16	ALA	ALA	ALA	ARG	ALA	ALA	GLN	LYS	ARG	THR

Samples:

sample_1: NS4B NTD, [U-13C; U-15N; U-2H], 0.6 ± 0.05 mM; 15N NS4B NTD, [U-100% 15N], 0.55 ± 0.05 mM; 13Cj 15N NS4B NTD, [U-100% 13C; U-100% 15N], 0.55 ± 0.05 mM; sodium phosphate 20 mM; LMPG 0.9%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCOCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1 - collection

NMRPipe - processing

CARA - data analysis

NMRViewJ - data analysis

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	ASN	GLU	LEU	GLY	TRP	LEU	GLU	ARG	THR
4	LYS	SER	ASP	LEU	SER	HIS	LEU	MET	GLY	ARG
5	ARG	GLU	GLU	GLY	ALA	THR	ILE	GLY	PHE	SER
6	MET	ASP	ILE	ASP	LEU	ARG	PRO	ALA	SER	ALA
7	TRP	ALA	ILE	TYR	ALA	ALA	LEU	THR	THR	PHE
8	ILE	THR	PRO	ALA	VAL	GLN	HIS	ALA	VAL	THR
9	THR	SER	TYR	ASN	ASN	TYR	SER	LEU	MET	ALA
10	MET	ALA	THR	GLN	ALA	GLY	VAL	LEU	PHE	GLY
11	MET	GLY	LYS	GLY	MET	PRO	PHE	TYR	ALA	TRP
12	ASP	PHE	GLY	VAL	PRO	LEU	LEU	MET	ILE	GLY
13	CYS	TYR	SER	GLN	LEU	THR	PRO	LEU	THR	LEU
14	ILE	VAL	ALA	ILE	ILE	LEU	LEU	VAL	ALA	HIS
15	TYR	MET	TYR	LEU	ILE	PRO	GLY	LEU	GLN	ALA
16	ALA	ALA	ALA	ARG	ALA	ALA	GLN	LYS	ARG	THR

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	ASN	GLU	LEU	GLY	TRP	LEU	GLU	ARG	THR
4	LYS	SER	ASP	LEU	SER	HIS	LEU	MET	GLY	ARG
5	ARG	GLU	GLU	GLY	ALA	THR	ILE	GLY	PHE	SER
6	MET	ASP	ILE	ASP	LEU	ARG	PRO	ALA	SER	ALA
7	TRP	ALA	ILE	TYR	ALA	ALA	LEU	THR	THR	PHE
8	ILE	THR	PRO	ALA	VAL	GLN	HIS	ALA	VAL	THR
9	THR	SER	TYR	ASN	ASN	TYR	SER	LEU	MET	ALA
10	MET	ALA	THR	GLN	ALA	GLY	VAL	LEU	PHE	GLY
11	MET	GLY	LYS	GLY	MET	PRO	PHE	TYR	ALA	TRP
12	ASP	PHE	GLY	VAL	PRO	LEU	LEU	MET	ILE	GLY
13	CYS	TYR	SER	GLN	LEU	THR	PRO	LEU	THR	LEU
14	ILE	VAL	ALA	ILE	ILE	LEU	LEU	VAL	ALA	HIS
15	TYR	MET	TYR	LEU	ILE	PRO	GLY	LEU	GLN	ALA
16	ALA	ALA	ALA	ARG	ALA	ALA	GLN	LYS	ARG	THR

1	MET	GLY	SER	SER	HIS	HIS	HIS	HIS	HIS	HIS
2	SER	SER	GLY	LEU	VAL	PRO	ARG	GLY	SER	HIS
3	MET	ASN	GLU	LEU	GLY	TRP	LEU	GLU	ARG	THR
4	LYS	SER	ASP	LEU	SER	HIS	LEU	MET	GLY	ARG
5	ARG	GLU	GLU	GLY	ALA	THR	ILE	GLY	PHE	SER
6	MET	ASP	ILE	ASP	LEU	ARG	PRO	ALA	SER	ALA
7	TRP	ALA	ILE	TYR	ALA	ALA	LEU	THR	THR	PHE
8	ILE	THR	PRO	ALA	VAL	GLN	HIS	ALA	VAL	THR
9	THR	SER	TYR	ASN	ASN	TYR	SER	LEU	MET	ALA
10	MET	ALA	THR	GLN	ALA	GLY	VAL	LEU	PHE	GLY
11	MET	GLY	LYS	GLY	MET	PRO	PHE	TYR	ALA	TRP
12	ASP	PHE	GLY	VAL	PRO	LEU	LEU	MET	ILE	GLY
13	CYS	TYR	SER	GLN	LEU	THR	PRO	LEU	THR	LEU
14	ILE	VAL	ALA	ILE	ILE	LEU	LEU	VAL	ALA	HIS
15	TYR	MET	TYR	LEU	ILE	PRO	GLY	LEU	GLN	ALA
16	ALA	ALA	ALA	ARG	ALA	ALA	GLN	LYS	ARG	THR