BMRB Entry 52622
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52622
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Chaudhari, Aditya; FAVIER, Adrien; Aliakbartehrani, Zahra; Koval, Tomas; Andersson, Inger; Schneider, Bohdan; Dohnalek, Jan; Cerny, Jiri; Brutscher, Bernhard; Fuertes, Gustavo. "Light-dependent flavin redox and adduct states control the conformation and DNA-binding activity of the transcription factor EL222" Nucleic Acids Res. 53, gkaf215-gkaf215 (2025).
PubMed: 40119733
Assembly members:
entity_1, polymer, 212 residues, Formula weight is not available
entity_FMN, non-polymer, 456.344 Da.
Natural source: Common Name: Erythrobacter litoralis Taxonomy ID: 39960 Superkingdom: Bacteria Kingdom: not available Genus/species: Erythrobacter litoralis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 359 |
| 15N chemical shifts | 171 |
| 1H chemical shifts | 171 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EL222 | 1 |
| 2 | FMN | 2 |
Entities:
Entity 1, EL222 212 residues - Formula weight is not available
| 1 | GLY | GLU | PHE | GLY | ALA | ASP | ASP | THR | ARG | VAL | ||||
| 2 | GLU | VAL | GLN | PRO | PRO | ALA | GLN | TRP | VAL | LEU | ||||
| 3 | ASP | LEU | ILE | GLU | ALA | SER | PRO | ILE | ALA | SER | ||||
| 4 | VAL | VAL | SER | ASP | PRO | ARG | LEU | ALA | ASP | ASN | ||||
| 5 | PRO | LEU | ILE | ALA | ILE | ASN | GLN | ALA | PHE | THR | ||||
| 6 | ASP | LEU | THR | GLY | TYR | SER | GLU | GLU | GLU | CYS | ||||
| 7 | VAL | GLY | ARG | ASN | CYS | ARG | PHE | LEU | ALA | GLY | ||||
| 8 | SER | GLY | THR | GLU | PRO | TRP | LEU | THR | ASP | LYS | ||||
| 9 | ILE | ARG | GLN | GLY | VAL | ARG | GLU | HIS | LYS | PRO | ||||
| 10 | VAL | LEU | VAL | GLU | ILE | LEU | ASN | TYR | LYS | LYS | ||||
| 11 | ASP | GLY | THR | PRO | PHE | ARG | ASN | ALA | VAL | LEU | ||||
| 12 | VAL | ALA | PRO | ILE | TYR | ASP | ASP | ASP | ASP | GLU | ||||
| 13 | LEU | LEU | TYR | PHE | LEU | GLY | SER | GLN | VAL | GLU | ||||
| 14 | VAL | ASP | ASP | ASP | GLN | PRO | ASN | MET | GLY | MET | ||||
| 15 | ALA | ARG | ARG | GLU | ARG | ALA | ALA | GLU | MET | LEU | ||||
| 16 | LYS | THR | LEU | SER | PRO | ARG | GLN | LEU | GLU | VAL | ||||
| 17 | THR | THR | LEU | VAL | ALA | SER | GLY | LEU | ARG | ASN | ||||
| 18 | LYS | GLU | VAL | ALA | ALA | ARG | LEU | GLY | LEU | SER | ||||
| 19 | GLU | LYS | THR | VAL | LYS | MET | HIS | ARG | GLY | LEU | ||||
| 20 | VAL | MET | GLU | LYS | LEU | ASN | LEU | LYS | THR | SER | ||||
| 21 | ALA | ASP | LEU | VAL | ARG | ILE | ALA | VAL | GLU | ALA | ||||
| 22 | GLY | ILE |
Entity 2, FMN - C17 H21 N4 O9 P - 456.344 Da.
| 1 | FMN |
Samples:
sample_1: EL222 lit2, [U-100% 13C; U-100% 15N], 200 uM; FMN, [U-100% 13C; U-100% 15N], 200 uM; MES buffer 25 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Best TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCOCA | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.5 - collection, processing
NMRlib v2.0 - collection
CcpNMR v2 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks