BMRB Entry 52619

Name: Backbone and side chain 1H, 13C, 15N Chemical Shift Assignments for S. cerevisiae SERF
Published: 2024-11-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52619

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side chain 1H, 13C, 15N Chemical Shift Assignments for S. cerevisiae SERF

Deposition date:

2024-09-27

Original release date:

2024-11-11

Authors:

Usher, Emery; Mitra, Rishav; Showalter, Scott

Citation:

Citation: Mitra, Rishav; Usher, Emery; Dedeoglu, Selin; Crotteau, Matthew; Fraser, Olivia; Yennawar, Neela; Gadkari, Varun; Ruotolo, Brandon; Holehouse, Alex; Salmon, Loic; Showalter, Scott; Bardwell, James. "Molecular insights into the interaction between a disordered protein and a folded RNA" Proc. Natl. Acad. Sci. U.S.A. 121, e2409139121-e2409139121 (2024).
PubMed: 39589885

Assembly members:

Assembly members:
entity_1, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MARGNQRDLARQKNLKKQKD MAKNQKKSGDPKKRMESDAE ILRQKQAAADARREAEKLEK LKAEKTRR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	260
15N chemical shifts	67
1H chemical shifts	128

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SERF	1

Entities:

Entity 1, SERF 68 residues - Formula weight is not available

1

MET

ALA

ARG

GLY

ASN

GLN

ARG

ASP

LEU

ALA

2

ARG

GLN

LYS

ASN

LEU

LYS

GLN

LYS

ASP

3

MET

ALA

LYS

ASN

GLN

LYS

SER

GLY

ASP

4

PRO

LYS

ARG

MET

GLU

SER

ASP

ALA

GLU

5

ILE

LEU

ARG

GLN

LYS

GLN

ALA

ASP

6

ALA

ARG

GLU

ALA

GLU

LYS

LEU

GLU

LYS

7

LEU

LYS

ALA

GLU

LYS

THR

ARG

Samples:

sample_1: SERF, [U-99% 13C; U-99% 15N], 0.9 mM; potassium phosphate 50 mM; potassium chloride 50 mM; sodium azide 0.01%; magnesium chloride 1 mM; D2O, [U-99% 2H], 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 13C-15N CON-IPAP	sample_1	isotropic	sample_conditions_1
2D 13C-15N (HACA)CON-IPAP	sample_1	isotropic	sample_conditions_1
3D (HACA)N(CA)CON-IPAP	sample_1	isotropic	sample_conditions_1
3D (HACA)N(CA)NCO-IPAP	sample_1	isotropic	sample_conditions_1
3D (H)CCCON-IPAP	sample_1	isotropic	sample_conditions_1
3D H(CC)CON-IPAP	sample_1	isotropic	sample_conditions_1
2D CAS-(CA)CON-ALA-IPAP	sample_1	isotropic	sample_conditions_1
2D CAS-(CA)NCO-ALA-IPAP	sample_1	isotropic	sample_conditions_1
2D CAS-(CA)CON-ASP-IPAP	sample_1	isotropic	sample_conditions_1
2D CAS-(CA)NCO-ASP-IPAP	sample_1	isotropic	sample_conditions_1
2D CAS-(CA)CON-GLU-IPAP	sample_1	isotropic	sample_conditions_1
2D CAS-(CA)NCO-GLU-IPAP	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 13C-15N CON-IPAP	sample_1	isotropic	sample_conditions_1
2D 13C-15N (HACA)CON-IPAP	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.0 - collection

TOPSPIN - processing

NMRFAM-SPARKY - peak picking

NMRFAM-SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz
Bruker AVANCE III 600 MHz