BMRB Entry 52613

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52613

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Title:
Chemical Shift Assignments of Disabled-2 320-495 (Dab2)
Deposition date:
2024-09-19
Original release date:
2025-08-18
Authors:
Papagiannoula, Andromachi; Vedel, Ida; Motzny, Kathrin; Tengo, Maud; Saiti, Arbesa; Milles, Sigrid
Citation:

Citation: Papagiannoula, Andromachi; Vedel, Ida; Motzny, Kathrin; Tengo, Maud; Saiti, Arbesa; Milles, Sigrid. "Promiscuous and multivalent interactions between Eps15 and partner protein Dab2 generate a complex interaction network"  Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 185 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKKENSSSSSTPLSNGPLNG DVDYFGQQFDQISNRTGKQE AQAGPWPFSSSQTQPAVRTQ NGVSEREQNGFSVKSSPNPF VGSPPKGLSIQNGVKQDLES SVQSSPHDSIAIIPPPQSTK PGRGRRTAKSSANDLLASDI FAPPVSEPSGQASPTGQPTA LQPNPLDLFKTSAPAPVLEH HHHHH

Data sets:
Data typeCount
13C chemical shifts446
15N chemical shifts139
1H chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Dab2 320-4951

Entities:

Entity 1, Dab2 320-495 185 residues - Formula weight is not available

1   METLYSLYSGLUASNSERSERSERSERSER
2   THRPROLEUSERASNGLYPROLEUASNGLY
3   ASPVALASPTYRPHEGLYGLNGLNPHEASP
4   GLNILESERASNARGTHRGLYLYSGLNGLU
5   ALAGLNALAGLYPROTRPPROPHESERSER
6   SERGLNTHRGLNPROALAVALARGTHRGLN
7   ASNGLYVALSERGLUARGGLUGLNASNGLY
8   PHESERVALLYSSERSERPROASNPROPHE
9   VALGLYSERPROPROLYSGLYLEUSERILE
10   GLNASNGLYVALLYSGLNASPLEUGLUSER
11   SERVALGLNSERSERPROHISASPSERILE
12   ALAILEILEPROPROPROGLNSERTHRLYS
13   PROGLYARGGLYARGARGTHRALALYSSER
14   SERALAASNASPLEULEUALASERASPILE
15   PHEALAPROPROVALSERGLUPROSERGLY
16   GLNALASERPROTHRGLYGLNPROTHRALA
17   LEUGLNPROASNPROLEUASPLEUPHELYS
18   THRSERALAPROALAPROVALLEUGLUHIS
19   HISHISHISHISHIS

Samples:

sample_1: Disabled-2 320-495, [U-99% 13C; U-99% 15N], 1.3 mM; D2O, [U-100% 2H], 10%; sodium phosphate 50 mM; sodium chloride 150 mM; DTT 2 mM

sample_conditions_1: ionic strength: 211.6 mM; pH: 6; pressure: 1 atm; temperature: 298.2 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D BT-HNCOsample_1isotropicsample_conditions_1
3D BT-HN(CA)COsample_1isotropicsample_conditions_1
3D BT-HN(CO)CAsample_1isotropicsample_conditions_1
3D BT-iHNCAsample_1isotropicsample_conditions_1
3D BT-HN(CO)CACBsample_1isotropicsample_conditions_1
3D BT-iHNCACBsample_1isotropicsample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

CcpNMR - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks